Journal of the Japan Society of Blood Transfusion
Online ISSN : 1883-8383
Print ISSN : 0546-1448
ISSN-L : 0546-1448
Volume 5, Issue 4
Displaying 1-10 of 10 articles from this issue
  • Mitsuo MAKITA, Katsuro SEKINE, Masato YAMADA
    1958 Volume 5 Issue 4 Pages 145-146
    Published: 1958
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    The classification of the mouse blood types was described and the following results were obtained.
    1. Applying human anti-A and anti-B sera as agglutinins, the blood cells of the mouse were divided into four groups:
    a) agglutinated by human anti-A sera (17.4per cent)
    b) agglutinated by human anti-B sera (13.9per cent)
    c) agglutinated by human anti-A and anti-B sera (48.2per cent)
    d) neither agglutinated by human anti-A nor anti-B sera (20.5per cent)
    2. The blood cells of some mice were agglutinated by human AB blood type sera (69.6per cent). This result indicated that human AB blood type sera included certain agglutinins against mouse blood cells.
    3. Based on the antigenic analysis of blood types of the mouse by means of anti M I and M II sera of immunized rabbits, the blood cells of mouse were divided into four groups:
    a) M I type (13.3per cent)
    b) M II type (40.9per cent)
    c) M I, M II type (43.8per cent)
    d) X (non-agglutinated) type (2.0per cent)
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  • Takehiko KAWANO
    1958 Volume 5 Issue 4 Pages 147-153
    Published: 1958
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    For the purpose of studying the influences of leukocytes on the preservation of blood cells, the author prepared blood samples containing more or less leukocytes from blood collected in ACD solution, by separating leukocytes as buffy coat and by removing leukocytes with serum albumin solution (human), the specific gravity having been adjusted to lie between those of erythrocytes and leukocytes, and observed the changes of the blood during storage at 4-6°C.
    1) Influence of leukocytes during storage was not noticeable on the changes of hematocrit value, agglutinability of erythrocytes, amino-nitrogen contents of plasma, fibrinolysis and amino-acids in plasma as observed by paper-chromatography.
    2) It was observed that leukocytes accelerated the decrease in the erythrocyte sedimentation rate, the decline of blood sugar level and the increase of osmotic fragility of erythrocytes or of free hemoglobin content in plasma during storage.
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  • Takehiko KAWANO
    1958 Volume 5 Issue 4 Pages 154-155
    Published: 1958
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    Changes of temperature in blood collected with ACD-solution (Formula B) in 250cc bottles were observed by a thermo-couple potentiometer
    1) in collection bottles were cooled in an ice-box of 4-6°C prior to the bleeding and in those not previously cooled, and
    2) in the collection bottles were immediately immersed in an ice-water pan prior to ice-box storage and in those without such preliminary cooling.
    In addition the author also investigated the time during which blood was kept at the temperature above 10°C, and concluded that the cooling effect of immersing the blood bottle in ice-water was striking for rapid cooling while the cooling of the blood bottle prior to the blood collection had little and rather negligible effect.
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  • Naotane YOSHINAGA, Tikara SEKIMOTO, Ken-itu SONODA, Tugihide TANAKA, Y ...
    1958 Volume 5 Issue 4 Pages 156-160
    Published: 1958
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    The authors made statistical observations on the incidence of post-transfusion jaundice during the period from January 1, 1954 to October 30, 1957 in 365 patients answering their questionnaires.
    1). The frequency of post-transfusion jaundice was 4.1per cent, the incubation period being 30-120 days (mean value: 65 days) and the duration of the disease 5-60 days (mean value: 39 days).
    2). No relationship was found between the morbidity and blood type or sex. The highest incidence was in individuals below 20 years of age, followed by those between 30 and 50 years.
    3). Both fresh and preserved bloods produced the disease and the difference between the two was difficult to estimate, since the use of fresh blood was made only in a few cases.
    4). The incidence seemed to be higher in transfusions of more than 4, 000cc of blood than in those of lesser amounts.
    5). The disease occurred more frequently in patients with tuberculosis who received large quantities of blood both before and after the operation, and less in those with gastric cancer.
    6). The prognosis was generally good and there were no fatalities.
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  • Mitsuo YOKOYAMA, Makoto TAKEUCHI, Kunihiko NAKADA, Carl COHEN
    1958 Volume 5 Issue 4 Pages 161-166
    Published: 1958
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    The present study was undertaken in an effort to systematize the knowledge of blood groups in the rabbit through utilization of a large colony of rabbits at the Jackson Memorial Laboratory by Carl Cohen. This report covers the first phase of the work, demonstrating the presence and the classification of blood group antigen of rabbits by using the anti-sera, A, (B), C, D, E, F, H and I respectively, which are prepared by Carl Cohen.
    The results of the present investigation are as follows:
    1) The iso-agglutinins were present in the serum of the rabbits tested in the ratio of 36 out of 154 (23.4%). The sera were compared by cross-matching.
    2) The agglutination titer in the serum of rabbits was very low at room temperature and the serum did not react at 30°C or higher. No hemolysis occurred in any sera.
    3) The blood grouping could not be used as a basis to determine whether or not given blood types would agglutinate. In some cases agglutination might have been the result of low temperature.
    4) This is the first investigation conducted in Japan in an attempt to classify the blood groups of rabbits by using the group specific anti-sera prepared by Carl Cohen. In this study the frequency of CF and ACF combination of blood groups was extremely high but the single type of blood was not detected in the present investigation except for F factor.
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  • Yosihiro OKADA, Joji SATO
    1958 Volume 5 Issue 4 Pages 167-171
    Published: 1958
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    For the purpose of examining the influence of a large blood transfusion after the surgery for pulmonary tuberculosis the observations on bleeding time, coagulation time, serum bilirubin and B. S. P. test were made.
    There was no interrelationship between the quantity of blood transfusion and bleeding and coagulation times.
    The incidence of serum hepatitis was not related to the quantity of blood transfusion nor to preoperative liver function.
    Many problems of the quantity of blood loss during the operation for pulmonary tuberculosis still remain to be solved.
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  • Bunshi SAKAGUCHI
    1958 Volume 5 Issue 4 Pages 172-182
    Published: 1958
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    The fate of each component of preserved blood was investigated in blood collected from 30 males in ACD-B solution during storage at 4°C. On every 5th day for 20 days the value of sugar in blood and plasma, total plasma cholesterol, plasma mucoprotein, total plasma protein, and each protein fraction and lipoprotein in serum were determined.
    1. Blood sugar declined in value rapidly during the first 10 days and slowly later, the value on the 20th day being significantly different from that on the first day. The value of plasma sugar was considerably higher than that of blood sugar on the first day, but it declined rapidly later untill it reached the same level as that of sugar on the 5th day. Both constituents decreased at the same rate from the 11th day on.
    2. Total plasma cholesterol, plasma mucoprotein, total plasma protein, protein fractions in serum, and plasma lipotrotein varied more or less in value in course of time, but the difference produced in the value of each blood constituent was not statistically significant.
    3. Paperelectrophoretic determination of each protein fraction in serum, and of alpha and beta fractions of lipoprotein in preserved blood, was more difficult than that of each corresponding constituents of normal fresh blood.
    4. It appears that the component of preserved blood, sugar excepted, are relatively invariable in quantity though they—protein in particular—are likely to undergo the same qualitative variations.
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  • I. CHANGES IN BLOOD COAGULABILITY FOLLOWING THE USE OF EXTRACORPOREAL CIRCULATION
    Kagemitu NINOMIYA
    1958 Volume 5 Issue 4 Pages 183-193
    Published: 1958
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    Clinical and experimental studies on the changes in blood coagulability were made in extracorporeal circulation by the aid of direct vision intracardiac surgery, consisting of selective brain cooling by irrigation and artificial heart-lung pump oxygenator. To prevent the postoperative bleeding tendency the author investigated the use of protamine to neutralize the anticoagulant effects of heparin as well as the postoperative fibrinolytic phenomenon.
    1) Heparin-protamine titration performed in the whole blood indicated the neutralizing ratio of 1:1.2.
    2) For determining the required doses of protamine a simple and reliable titration method was devised.
    3) Single and slow infusion of a relatively small dose of protamine exhibited satisfactory effects.
    4) The heparin rebound phenomenon was not observed and the additional injection of protamine was not required.
    5) The estimation of plasma fibrinogen levels and the thrombelastograph in tracings revealed no fibrinolytic phenomenon.
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  • Tamotsu Fukuda, Tomio Ogata
    1958 Volume 5 Issue 4 Pages 194-197
    Published: 1958
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    Download PDF (641K)
  • 1958 Volume 5 Issue 4 Pages 198
    Published: 1958
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
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