In order to measure the thermal diffusivity of multi-layered material by a laser flash method, analyzing method of the thermal diffusivity of any layer was investigated using the multi-layered sample temperature equation obtained in the Laplace space under the radiation loss. First method is a search of a root-mean-square deviation minimum in the Laplace space under the condition that the thermal diffusivity and the Biot number are independent variable (Direct method). Second method is a method to search the minimum under the condition that the theoretical cooling time constant equals to the cooling time constant of the measured temperature data of real space (Cooling time constant method). It was confirmed that it is possible to determine the thermal diffusivity and Biot number by both methods and the thermal diffusivity can be analyzed by Direct method using data in short measurement time.