Liver flukes are highly parasitic on sika deer in Japan, although it is not highly on livestock such ascattle and sheep. Recently, sika deer are remarkably increasing in Hokkaido, Japan. Therefore, liver flukes may spread more in Hokkaido as sika deer expand their range. Liver flukes may also expand to livestock and to human. To maintain livestock industry and human health, it is important to understand the current situation of liver fluke parasitism of sika deer. We investigated frequency sika deer are parasitized in the Tokachi District, a dairy farming area typical to Hokkaido. We collected 507 feces samples of sika deer from 10 study sites in Tokachi from May to October, 2012. We examined these feces for liver fluke eggs. Prevalence in Tokachi was 14.2%. In each study site, we compared the prevalence of sika deer with that of cattle reported by the Hokkaido Obihiro Meat Inspection Center. In some study sites, both species had liver fluke. To prevent liver flukes from spreading to live stock and humans, further survey of liver fluke parasitism of sika deer is needed.
Gross and SEM observation on the tongue of a newborn Cuvier's beaked whale (Ziphius cavirostris) was carried out. This research observed a spoon-shaped tongue, three types of marginal papillae on the anterior lateral part of the tongue and several deep grooves on the both sides of the middle region of the tongue. After removal of the epithelium, it was revealed that the conical connective tissue cores (CTC) were distributed densely on the surface of the tongue. The CTC of the marginal papillae consisted of a main protrusion and numerous small rod-shaped secondary cores. The CTC of the grooves were elongated in shape. These were the morphological characteristic of the tongue of a newborn Cuvier's beaked whale.
54 raccoons (Procyon lotor) with dental caries-like lesions (hereafter referred as “dental caries”) were caught in Chitose City, Hokkaido, and their age estimation and dental caries diagnosis were performed. Dental caries were observed with high frequency on the posterior teeth, and those of the molar teeth were particularly serious. Dental calculus was observed for 49 raccoons, mainly on the molar teeth as was the case for the dental caries. About 60% of the individuals with dental caries were observed with the age between 2.5 and 4.5, and the proportions of the younger (<1.5) and the elder (>5.5) individuals were low. Furthermore, the dental caries of the raccoons tended to be more rampant with increase of age.
A 31-year-old male spotted seal (Phoca largha) was examined at necropsy. Multiple masses were observed in systemic organs, including the lung, stomach, pancreas, greater omentum, and pulmonary lymph nodes. Histologically, pulmonary masses contained numerous solid nests composed of polygonal tumor cells and often central keratinization, whereas those were less keratinization in the stomach, which tumor emboli were observed in the vessels. On immunohistochemistry, tumor cells were positive for keratin 5, pancytokeratin AE1/AE3, and p63 but negative for vimentin, cytokeratin CAM5.2, and calretinin. Based on macroscopical, histological, and immunohistochemical features, this case was diagnosed as squamous cell carcinoma with systemic metastases. The primary site in this case was probably the lung, with vascular metastases to distant organs including the stomach, omentum, and pulmonary lymph nodes. To the authors' knowledge, this is the first description of squamous cell carcinoma in a spotted seal.