Studies are made on various factors effectively influencing the production of fluorescin, pyocyanin and melanin of Pseudomonas aeruginosa as well as the productive aspethereof, with the following results:
1) A slant agar medium consisting of a synthetic medium added by 0.3-0.5% peptone is found to be very favorable in determining the kinds of the pigments which Pseudomonas aeruginosa produces. During the determination, fluorescin is diffused over the medium, melanin is observed on the uppermost layer of the medium, and pyocyanin is found on the next lower layer as a band.
2) Four sugars, glycerol, glucose, fructose and mannit in various combinations are studied as to their effects on the pigment production of Pseudomonas aeruginosa. It is revealed that each pigment is referable to its specific amino acid and that each combination of sugars results in its specific productive aspects.
3) Numerous amino acids uniformly take part in the production of fluorescin and melanin; only a small number of amino acids does in the production of pyocyanin.
4) No amino acids or sugars considered essential for the production of pigments are scarcely observed. But many amino acids and sugars are found to participate in the production rather favorably. The degree of such favorable participation is dependent upon the conditions under which sugars and amino acids are combined.
5) What is considered to belong to pyocyanin strain and what is considered to belong to melanin strain are possessed with the ability of producing pyocyanin, melanin, and fluorescin. It seems that only in the amount of pigment production the former is different the latter. It is obvious that the amount is influenced to some extent by the environmental conditions under which these strains grow.
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