The auther has discussed “systematic method of hemostatic mechanism examinations”. Many hemostatic mechanism examinations are divided into screening and detailed examinations. The former is conducted collectively, its result is judged exactly, and then determined the presence of hemorrhagic disease and the type. After that the latter is carried out selectively, and make a correct diagnosis. The reasonable examination of hemostatic mechanism should be made effectively. It is also important to perform different examinations of the same kind repeatedly not to make a wrong diagnosis. Thromboelastograhy plays an important role in the screening examination.

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Three red pigments have been produced from Ps. aeruginosa, strain No.145, in the synthetic medium containing glutamic acid.
A pigment P-I has been isolated in pure state and its structure has been supposed to be a new derivative having a aminophenazine skeleton with the formula C₁₇H₁₉N₃O₉.
P-I can not be extracted from the culture with usual organic solvents and has been isolated using, column chromatography on the cross linked dextran gel, Sephadex G-25M and aluminium oxide treated with acid.
P-I concentrated to the top zone in a Sephadex column, and is not adsorbed on the acid washed alumina.
The red pigment, P-I isolated as crude crystals from the culture filtrate has been finally recrystallized several times from distilled water. The original culture contains about 1 mg of the purified P-I per liter.
The purified material has been obtained as deep purple red, needles(m. p. >250°C, dec.) and it is characterized by a beautiful green color reaction with concd. sulfuric acid. This reaction has been known as a common color reaction for aminophenazine derivatives.
Anal: Calcd. for C₁₇H₁₉O₉N₃. C 49.88, H 4.68, N 10.27. M. W.409.35. Found: C 49.80, H 4.54, N 10.35.
P-I is soluble in water, insoluble in non-polar solvents, dialysable, unstable in alkaline solution, reduced to yellow substance which is reoxidized with hydrogen peroxide or with air to. original pigment and considerably stable in aq. solution at pH 3-10. The acidic solution shows a reddish violet and a yellow color in alkaline solution. pKa = 3.14, Distance of Electromigration: + 0.5 (McIlvaine's buffer pH 6.0,0.73mA/cm,5 hrs.)
The ultraviolet and visible absorption spectrum in distilled water exhibits four maxima E^1%_1cm 1480 at 234mμ, E^1%_1cm 2040 at 283 mμ, E^1%_1cm 500 at 396 mμ and E ^1%_1cm 584 at 515 mμ, these are shifted to 236mμ,287mμ,390mμ,546mμ in 0.1N-HCl solution and 233 mμ,271mμ,374mμ,460mμ in 0.1 N-NaOH solution respectively.
The nuclear magnetic resonance spectrum of P-I suggests a existence of following groups; τ=7.08(-H-CH₃)and τ=8.18(CH3_??_).
In the infrared absorption spectrum, aromatic signals (1635,1520 cm^-1) and a broad peak of amino group (3400 cm^-1) are observed.
From results mentioned above, P-I was supposed to be one of amino-phenazine, However, by the comparison with several phenazines of the absorption spectrum over the range 220-600mμ the spectrum of P-I has been found to be very similar to that of 2,6-diaminophenazine. It is presumed that the red pigment has a phenazine structure substituted at C₂ and C₆ with some auxiliary chromophores.

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Spa was administered orally in rats and observed in respect of chronic toxicities. The results obtained are as follows:
1. Body weight gain was scarcely influenced by the prolonged administration of Spa at 5 or 10mg/kg orally once a day for 5 or 14 weeks.
2. After those administrations of the drug, hematological, anatomical, and histological findings showed slightly the significant pathological changes attributable to the

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Pathognomonic action of male sex hormone upon the submaxillary gland of mice was found by Lacassagne, who presented at first the sexual dimorphism of the ot he r organs than the reproductive organs. Unexpected effect of sex hormones upon the other organs than the reproductive organs are not very prominent but interestin g, and especialy the effect of sex hormones upon the metabolic organs should be disc ussed because of their metabolic actions. Morii et al. suggested that the metabolic action of androgen would induce sexual dimorphism of the submaxillary glands of mic e. Aragaki, Nakagawa & Morii pointed out that a prolonged administration of a ndrogen was followed by the distinct histological alterations of the exlacrymal gland of rats, and that this specific effect of androgen was detected even on the hypoph ysectomized rats. They supposed androgens induced in general a hyperplastic state of the glands. Gabe reported that sex hormones affected apparently the structur e and function of the exlacrymal glands, and indicated the cellular vacuolization and the nu clearpolymorphism on the glands of androgenized male rats. Minami observed that the gland of females was heavier than that of males, and that hypophysectomy was followed by an atrophy of the glands. But, male rats in her experiments had heavier exlacrymal glands in absolute number than the females. It was considerable that the body weight of her male rats was too heavy to correct the relative weight of the gland. In nuclei of acinar cells of the exlacrymal glands of rats, specific stimulation with androgens was reported by Italian authors. Recently, Yamada has studied on sex difference of the exlacrymal glands of rats. Her experimental results indicated that the sex difference exhibited prominently in the functional state, and that hyperfunctiona l state seemed to be followed only by slight hypertrophic findings. She has also described a follicular structure of the exiacrymal glands of rats receiced with a prolonged administration of androgen, and speculated that this type would be an exhaustive appearance, which was a final demonstration of the oversecretion of mesocrine type. Quintarelli et al. have obseved the effect of androgen on the exiacrymal gland of mice. Repeated injections of testosterone proponate (1 mg daily, for 50days) have determined some pronounced modifications of the galnds. The modifications were not only characterized by cytoplasmic vacuoles and an appearance of glycoprotein materials, but also 4 or 5 times enlargement in size.

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Five cases of double cancers were observed upon the 27 autopsy- cases of lung cancer in recent 8 years.
The 1st case: The grove-sized, squamous cell carcinoma originated from the right bronchus was found out of the man,68 year old, died of adenocarcinoma of the stomach with lymphogenous metastasis. Hamartomas were also detected in the esophagus, the duodenum, and the left renal cortex.
The 2nd case: The thumb-sized adenocarcinoma of the stomach with lymphogenous metastasis was detected in the man,62 year old, died of the goose egg-sized, anaplastic polymorphocellular carcinoma in the central part of the upper lobe of the right lung with lymphogenous and hematogenous metastasis. He was suffered from vena caval superior syndrome.
The 3rd case: Both the sparrow egg-sized, anaplastic cell carcinoma in the subpleural area (S2) of the right lung with lymphogenous and hematogenous metastasis and the thumb-sized, columnar cell carcinoma of the ijejunum were observed upon the man,80 year old, died of lobar pneumonia and arteriosclerotic nephrosclerosis. He was also suffered from the luetic aortic aneurysma.
The 4th case: The over chickin egg-sized, anaplastic small roundspindle cell carcinoma developed from the left lower bronchus with the mediastinal metastasis was observed upon the man,67 year old, suffered from vena caval syndrome. Rice -sized adenocarcinoma with no metastasis was detected in his thyroid gland.
The 5th case: Both the goose egg-sized, anaplastic polymorphocellular carcinoma induced from the right lower bronchus with hematogenous and lymphogenous metastasis and the thumb-sized, oat cell carcinoma in the subpleural area (S6) of the left lung with the left fulminating carcinomatous pleurisy were observed upon the man,79 year old, having small adenoma in the thyroid gland.
All 5 cases were the high-aged men having the chronic pulmonary alterations. It is suggested that the individual disposition e. g., sex and organ etc, would be important especialy for the causal genesis of double cancers.

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The relationship between tuberculous alterations in the lungs and malignant tumors was reviewed upon the 370 autopsy-materials on the more than 1 year old-persons in recent 8 years (1958-1965).
Out of the 370 cases, tuberculous alterations in the lunges were detected in the 74cases showing 13 exsudative,28 productive or 33 stational pulmonary tuberculosis. In the materials,208 malignant tumors including 9 double cancers were found out of 199 cases. The biggest number of the malignant tumors was 47 stomach cancers, and the second number 28 bronchial carcinomas.
Thirty-fourm alignant tumors were detected in the 74 cases with lung tuberculosis, and 174 malignant tumors in the 296 cases without lung tuberculosis. Bronchial carcinomas were detected in 11 % of the cases of pulmonary tuberculosis, and in 7%of the cases without pulmonary tuberculosis. Tuberculous changes in the lungs were observed on 7 cases out of 27 cases of bronchial carcinoma (26 %), and on 67cases out of 343 cases with no bronchial carcinoma (14 %). The combination rates between bronchial carcinoma and pulmonary tuberculosis would be too high to indicate the complication of the both to be accidental.
Upon the 208 malignant tumors, the complication of tuberculous alterations with malignant tumors was detected in the lungs of 9 % of the cases with tumors, tuberculous alterations without malignant tumors in the lungs of 16 %, malignant tumors without tuberculosis in the lungs of 25 %, and neither tuberculous changes nor malignant findings in the lungs of 55 %.
Histological appearances of the 8 bronchial carcinomas associated with pulmonary tuberculosis were classified to 6 squamous cell carcinomas and 2 anaplastic cell carcinomas suspected to a scar cancer. Upon the histopathological types of the 8 pulmonary tuberculosis with bronchial carcinoma,2 cases of exsudative and miliar form,2 cases of productive and sclerotic form and 4 cases of old and stational form were determined.
It was discussed that the general and local factors in the developments of the both pathological processes would be influenced by each other in addition to the histogenetic factor of malignant tumors in the inflammatory process.

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Urinary specimens obtained from a pregnant woman (27 years old,7th. month of gestation), a menstruating woman (19 years old, on the 11th. day of the cycle)and a postmenopausal woman (54 years old) as well as pooled from 5 adult men (between 29 and 31 years of age, mean of 29.4 years) and from 6 children (between 1.5 and 5 years of age, mean of 3.6 years) were extracted and purified in accordance with Brown's method (1955)followed by development of fluorescence by means of heating with 70% sulfuric acid. Photofluorimetric examinations of the interfering fluorescence was performed on the fluorescence spectra and activation spectra. In addition, urine extract prepared by Brown's method from 30 children (between 1,5and 5 years of age, mean of 3.6 years) was also developed fluorescence by 70%su1furic acid method Its fluorescence was excited with exciting radiations of 436mμand 365mμ of wavelength. The K value of the following correcting equation was determined according to the intensity of fluorescence measured using a photocell filter with maximum transmission in the region from 480mμto 530mμ.
Corrected Reading=unknown R₄₃₆mμ-KR₃₆₅mμ/standard R₄₃₆mμ-K. R₃₆₅mμ
The results are summarized as follows:
1) The maximum fluorescence intensities of the estrone-methyl ether(EO-m), estriol-methyl ether (ET-m) and estradiol-methyl ether (ED-m) were observed at 490,490 and 500 mμ of wavelength respctively. The optimum exciting radiation was found to be 470 mμ for all of the three.
2) For the each fraction of EO-m, ED-m and ET-m in the urine extract, the larger interference of fluorescence was seen in the lower concentration of such fraction, so that the interference was highest in children' s urine followed by in the order of postmenopausal female's, male's and menstruating female's urine. Almost none other than interfering fluorescence was thus observed in children's urine. Besides that, relatively more intermixture of fluorescent interfering substances were found in ED-m fraction than in EO-m and ET-m fractions.
3) The main interfering fluorescence was able to classify into 4 different varieties, each of which having the optimum exciting wavelength at 360,400,430 and 500mμ. Among them, the largest amount was detected at 360mμ.
4) The constant K of the correcting equation calculated by 30 cases of children's urine was 0.582±0.066 for EO-m,0.484±0.083 for ED-m and 1.275±0.068 for ET-m fraction.

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Venostasin, a drug extracted from Aesculus hippcastanum L., has pharmacologically recognized effects such as inhibtion of edema, strengthening of blood vessels or improvement of their fragility without exerting any influence on the blood coagulation. These effects are mainly ascribed to the constituents of the drug, i. e., “Ascin” (a saponin body) and various flavon glycosides resembling to quercitrin. Venostasin for injection 5 ml contains 150 mg extract of Aesculus hippcastanum and thyamine nitrate (V. B₁). It shows almost neutral pH.
In this paper, the authors report on the results obtained by administration of the drug (5-10 ml intravenously mixed with 20% glucose) to the patients with lung tuberculosis, blood disorders or liver diseases. Most of the patients had hemorrhagic tendency. Tests for hemorrhagic tendency were carried out successively, including measurement of the capillary resistance (C. R. ) by the petechiometer (Õkubo-Okamoto).
C. R. value came up in 21 cases out of 22, in concomitance with the improvement of the hemorrhagic symptomes by the administration of the drug. The one case which showed no elevation of C. R. had normal C. R. value befor the start of the administration. In the major part of the cases, the effect continued at least seven days after the cessation of the treatment (3 - 14 days administration), as detected by C. R. examination. Rumpel-Leede phenomenon and bleeding time were also improved by the treatment; while coagulation time and platelet count showed no remarkable change. Therefore, the effect of venostasin on the hemorrhagic disorders may be ascribed to the improvement of the blood vessel fragility.
Successive C. R. examination at short intervals revealed that the improvement of capillary fragility appeared in 6 - 8 hours after an intravenous injection of 5 ml venostasin, and that the effect was still maintained 24 hours after the injection.
No untoward reaction was observed, except for transient exanthema in one of the cases.
The authors, in conclusion, appreciate the remarkable hemostatic potency of venostasin, and they expect that some improvements of the dosage schedule may bring out the levetion of effects of the drug.

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