Some of butyrophenone derivatives, the third category of excellent psychotropic agents, were examined from the clinical view-point. The specific properties of these drugs consisted in stronger neuroleptic action than that of phenothiazine derivatives or of reserpine, also in poor involvement of autonomic nervous system and as well in prominent neurodysleptic action (Divry). This neurodysleptic action is divided into two types: major paro ysmal syndrome and minor subacute manifestation (restlessness syndrome). The latter syndrome is said very important and unique, as it is sometimes effectively utilized to crack “the shell of autism” of schizophrenic patients.
In our experiments, benperidol, haloperidol, dipiperon and trifluperidol (and spiroperidol shortly after this report) were given per os chiefly to schizophrenic patients, and the results and comparison among them were described hereafter.
Dipiperon and benperidol tended to ameliorate disturbance of sleep, while haloperidol and trifluperidol seemed often to bring disturbance of sleep in agitated state due to neurodysleptic action. Intravenous application of haloperidol and trifluperidol, however, brought sleepiness clinically as well as in EEG findings. Major dystonic syndrome was often observed in case of haloperidol and benperidol, while minor syndrome was more frequently observed in case of trifluperidol. The neurodysleptic action of dipiperon was milder than the others, but it seemed to possess thymoleptic action at the same time.
These butyrophenone derivatives, except dipiperon, were quite effective on paranoidhallucinatory state and psychomotor excitation of both acute and chronic psychoses. Main application of these drugs was, in our opinion, as follows: haloperidol was quite effective for mania, acute psychoses (implying schizophrenia), trifluperidol for confusion and both acute and chronic schizophrenia, benperidol for acute psychoses and dipiperon for atypical psychoses and for the beginning stage of schizophrenia. At the same time, effectiveness of these drugs was recognized in epileptic patients.
As to the side effect, extrapyramidal manifestations, neurodysleptic action, certain symptoms of autonomic nervous system were observed. But these side effects were easily removed by antagonistic agents.
Finally, it is always necessary to consider dosage, kind, side effects, etc. quite cautiously when usage of these of these drugs is enterprised, as the personal susceptibility to these drugs differs to pretty high extent.
Appendix: Clinical effect of spiroperdol is said to situate between that of holoperidol and trifluperdol.

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The production of water-soluble, diffusible, red pigments from Ps. aeruginosa, strain No.145, maintained in our laboratory, have been previously reported.
The final color demonstrated in liquid or solid medium of a pigment producing strain, varies with a kind of its medium. Most of these strains produce yellow pigments at initial stage of thier pigmentation.
The culture of strain No.145, also gave a yellow color, but no fluorescent on the surface of its slant-fluid during the first several hours of incubation and in the deep-fluid culture within 72 hours.
This yellow color rapidly disappeared, and then turned to the red. In order to know the nature of this yellow pigment a study was first directed to its isolation.
The yellow compound having its absorption maximum at 440 mμcould be separated from the culture supernatants by column chromatography on Sephadex G-25 M.
A fractionated yellow pigment characterized as PII was converted to red with treating of some oxitdizing reagent such as hydrogen peroxide, but no changed with air. Moreover, this yellow pigment was decolorized by alkali and turned to deep yellow in acidic solution. On the other hand, yellow-culture supernatants containing PI pigment was gradually changed to the red with airation.
Yellow pigment showed a similar ultraviolet absorption spectra with that of PII and its oxidation product (red pigment) gave the same migration of electrophoresis with PII. And these three pigments have a specific absorption given by sixmembered nitrogen containing hetero-cyclic compounds respectively. As a precursor the yellow pigment have a close relationship to the PII, judging from thier appearance during fermentation process and comparison of thier properties.
From these results, this yellow pigment could be supposed as an intermediate of the red pigment. The yellow pigment might be related to PII as its precursor.
In the previous paper, it was reported that the red pigment designated as PI was supposed to be one of aminophenazine derivatives but not to be Aeruginosin isolated by F. G. Holliman. It is presumed that most of the red pigments produced by Ps. aeruginosa are probably phenazine derivatives having some different, auxiliary chromophores.

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We experienced the thrombotest as for the control of the anticoagulant therapy, and the conclusion of our studies are as follows:
1) This method is simple and easy to do and is sensitive enough to find out the changes of the coagulation activity of the adsorbable factors both in the intrinsic and extrinsic systems.
2) It needs only a few equipments and the test can be done at the patient's bedside using small amount of capillary blood.
5) The test should be done daily during the first week of medication and once a week thereafter.

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In this report, a case of thirty-three years-old woman with an unusal course of pulmonary tuberculosis was presented, who received adrenocortical steroids and antibiotics (Sigmamycin, Erythromycin, and Chloramphenicol) without antituberculous drug therapy, because she was diagnosed uncorrectly as streptococcal pneumonia at first. Her general conditions improved with disappearance of fever and sputum after two month of the treatments, although her chest roentgenogram still showed the multiple nodular shadows in the left lung.
The histological examination of the resected left lower lobe after the treatment showed the finding of remarkable fibrosis in tuberculous foci, although the antituberculous drugs were not used.

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It is generally accepted that the anesthetizing time of rabbits by barbiturate is prolonged when a certain amount of cardiazol is administered.
In this experimentation, we studied the effects of these drugs on the action potential in the central nervous system.
The arousal reaction both on the cerebral cortex and hippocampus which develops following the stimulation of the brain stem reticular formation was not affected by cardiazol administration (10 mg/kg, i. v. ) after barbiturate (10 mg/kg, i. v. ).
Following the above-mentioned experiment, we examined the effects of drugs on recruiting response which appeared after the stimulation of then. centrum medianum and then. ventralis anterior.
It was revealed that recruiting response to these nuclei showed different susceptibility according to the agent administered. A series of experiments of n. centrum medianum have demonstrated a trend of increase in recruiting response following the administration of cardiazol. However, recruiting response in the case of the n. ventralis anterior was depressed after cardiazol administration.
From the above results, it may be safely asserted that what directly concerns the loss of senses is n. centrum medianum, and not n. ventralis anterior, and that n. ventralis anterior plays an important role in the development of recruiting response wave; in other words, that function is apart from wave form.

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It is expected from the results by Tashiro & Siekevitz (1965) and Tamaoki &Miyazawa (1967) that E. coli ribosomes may dissociate into ribosomal subunits through the intermediate stage. To confirm the above possibility, we have examined the dissociation and association of E. coli ribosomes by sucrose density gradient using 0.1 mM crude extract and purified 100S ribosomes.
60S component and the dimer of derived 30S ribosomal subunits (intermediate components) were frequently observed in association of ribosomal subunits and the former seemed to be swollen 70S ribosomes weakly bound. These intermediate components were also observed in dissociation of 100S ribosomes, but it was only a few cases and most of them were observed when purified 100S ribosomes were layered onto a sucrose density gradient made with 10 mM Tris buffer containing 1 mM or 0.5 mM Mg²⁺ without dialysis and immediately centrifuged. In this case, we can't deny the possibility that 70S ribosomes may contaminate in the 50S particle region as a swollen 70S ribosome, because 70S ribosomes swell according to incomplete dissociation under the experimental conditions, and thereby the friction coefficient increases, which results in the decrease of the sedimentation coefficient of the swollen 70S ribosomes. The process of association and dissociation of E. coli ribosomes has been discussed and posturated.

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The First Department of Physiology (Supervised by Prof. Yutaka Tashiro), Kansai Medical School We have previously shown that 60S component and the dimer of D-30S ribosomal subunits may exist in the course of dissociation from and association to 70S ribosomes of E. coli. To obtain more direct evidences for the possibilities, we have examined the intPrartinn between islated ribosomal subunits by sucrose density gradient centrifugation and obtained the following results: (a) Among D-50S, D-30S, N-50S and N-30S ribosomal subunits, D-30S ribosomal subunits only can combine together to form the dimer with the sedimentation coefficient of 50S, (b) N-50S ribosomal subunits can't combine with both N-30S and D-30S ribosomal subunits, (c) N-30S ribosomal subunits can combine with derived 50S ribosomal subunits to form various forms of particles corresponding to about 40S,45S,50S,60S,64S and 72S, (d) D-50S and D-30S ribosomal subunits can combine together to form 70S ribosomes but their combining ability decreases by purification and (e) 60S component seems to be swollen 70S ribosomes weakly bound. The combining character of ribosomal subunits has been discussed.

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