The following results have been rendered reliable by means of the iron hematoxylinsudan III staining method, several histochemical dehydrogenase detection methods, and trypan blue as well as neutral red vital staining methods.
On the 3rd day after enucleation of the adrenal gland, hyperemia in the capsule of the adrenal cortex and proliferation of fibroblast-like cells in the innermost side of the capsule were recognized, but no cortical cell regeneration could be found until the 5th day after enucleation when 3 β-ol dehydrogenase and G-6-P dehydrogenase activities become particularly noticeable in the regenerating cells.
The new adrenocortical cells regene rate forming a fasticular structure divided off by the connective tissue cord towards the central part of the adrenal gland up until about the 30th day after enucleation.
Moreover, the fact that among these cells at this stage of regeneration, cell groups with relatively few neutral red pigment assuming a glomerular structure, appear in the subcapsular region, may be regarded as constituting a new finding to support the opinion that the glomerular zone would be found during this stage.
The various enzyme activities at this stage have been found to display considerable differences between cell groups in the subcapsular region corresponding to the glomerular zone and those assuming a fasticular structure, on the other hand, the difference in the form of arrangement between each of the two groups of cells corresponds approximately to that between these two zones in nontreated group, though to a less remarkable degree.
These facts may be sufficient to suggest that the newly regenerated adrenocortical cells during the initial several days after enucleation, remain functionally and morphologically undifferentiated, but those from the 20th to the 30th day after enucleation, assume differentiation in their morphology. Moreover, judging from occurrence of cell groups undoubtedly not only morphologically but also histochemically corresponding to the glomerular zone, from the 40th to the 100th day after enucleation, it may be presumed that the functional and morphological differentiation of regenerated adrenocortical cells can proceed so far as to perfect a recreation of the enucleated adrenal cortex.
The problem does remain, however, that even at this stage neith er the transitional nor the reticular zone has yet appeared, and that the medullary part of the adrenal gland remains wholly lacking.
The newly re generating adrenocortical cells are considered capable of producing corticosteroids as early as the 5th day after enucleation, because of the remarkable activity of such enzymes as steroid 3 β-ol dehydrogenase and G-6-P dehydrogenase at that time.
Accordingly, it is considered that the regenerated cells compensate the adrenoco rticosteroid secretion which is supposed to occur soon after enucleation of the adrenal gland.
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