巻号一覧
28 巻 (1976)
- 4 号 p. 761-
- 3 号 p. 507-
- 2 号 p. 251-
- 1 号 p. 1-
- Supplement 号 p. S1-
前身誌
28 巻, Supplement 号
選択された号の論文の7件中1～7を表示しています
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Tokuji Nogawa, Katsuyuki Katayama, Yoshio Tabata, Tatsuichiro Ohshio, ...1976 年 28 巻 Supplement 号 p. S1-S20
発行日: 1976/12/20
公開日: 2013/02/19
ジャーナル フリーA digital method for the estimation of amplitude and phase of the EEG is introduced on the basis of the in-phase and quadrature-phase digital band-pass filters. Applicability of this method to measurements of the EEG changes due to a single photic stimulation is investigated in comparison with the method of complex demodulation. Both methods are found to be useful for measurements of changes in the alpha activity and found to provide similar results. A relation between the filter band-width and the estimated amplitude or phase variations is examined. An attempt is made to detect the peak frequency shifting due to a single photic stimulus.抄録全体を表示PDF形式でダウンロード (2191K) -
Toshio Yamashita1976 年 28 巻 Supplement 号 p. S21-S26
発行日: 1976/12/20
公開日: 2013/02/19
ジャーナル フリーPDF形式でダウンロード (763K) -
Motohiro Yasuhara, Hiroe Naito, Yonekazn Tachibana1976 年 28 巻 Supplement 号 p. S27-S37
発行日: 1976/12/20
公開日: 2013/02/19
ジャーナル フリーPDF形式でダウンロード (3175K) -
Takuya Kitajima1976 年 28 巻 Supplement 号 p. S37-S61
発行日: 1976/12/20
公開日: 2013/02/19
ジャーナル フリーInfluence of the dietary Indole upon the tumorigenesis with DBN was investigated in Wistar male rats. DBN was either suspended (0.05w/v%) in the drinking water or dissolved (2w/v%) in sesame oil. Indole was supplemented (1.6%) with the basal diet. Both Group 1 fed on the basal diet and Group 2 fed on the basal diet supplemented with Indole were given freely the drinking water containing DBN. Both Group 3 fed on the basal diet and Group 4 fed on the basal diet supplemented with Indole were injected subcutaneously by DBN,50 mg/kg, twice a week for 24 weeks. Experimental rats were given the special water or the special diet in the time between 6-28 weeks of age. They were sacrificed in the age of 28-40 weeks.
The incidence of esophageal tumors was 100% in Group 1,70% in Group 2,60% in Group 3, and 5% in Group 4, respectively. While that of vesical tumors was 50% in Group 1,20% in Group 2,73% in Group 3, and 30% in Group 4, respectively. As the hepatic tumors had developed in all experimentally effective rats for DBN-tumorigenesis, there was no difference from each other group. Both higher incidence of esophageal tumors in the rats drunk with DBN and lower incidence of esophageal tumors in rats injected by DBN would indicate the direct action of DBN on the esophageal mucosa. Dietary Indole inhibited prominently, the tumorigenesis with DBN in male rats. Therefore, a competition between DBN and Indole might be speculated in the tumorigenesis of the target tissues.抄録全体を表示PDF形式でダウンロード (15630K) -
I. Factors Influencing Plaque Formation of Japanese Encephalitis VirusJian-Yi Hsue1976 年 28 巻 Supplement 号 p. S62-S86
発行日: 1976/12/20
公開日: 2013/02/19
ジャーナル フリーThe conditions under which Japanese encephalitis virus plaques are formed on LLC-MK2 cell were studied in detail. The conditions concerned were: cellular sensitivity, virus strain, cell aging, washing of the cultured cells, adsorption time, diluent effect, culture temperature, concentration of fetal calf serum, methyl cellulose, sodium bicarbonate and trypsin.
Various vertebrate cell lines were screened for plaguing efficiency. Vero, LLC-MK2, FL and chick embryo cells all appeared to give comparable viral titers. The LLC-MK2 cell was suitable for JE virus plaque assay, because of good growth and easy subcultivation. It has been shown that the optimal conditions for JE virus plaque formation in LLC-MK2 cells were: (i) Incubation temperature of 37°C. (ii) Viral adsorption duration of 60 minutes. (iii) Hanks' balanced salt supplement with 0.5% bovine plasma albumin at pH 7.2 as diluent of viral samples. (iv) Employment of overlayer medium containing 1.5% methyl cellulose and incorporated with 3 % inactivated fetal calf serum, NaHCO3 2.8 mg/ml. (v) Duration of 7 days for development of plaque. (vi) Mg and Ca cations required for maximal virus adsorption but plaques produced in the absence of Ca and Mg cations. (vii) Trypsin, virus strain, cell age and washing of the monolayer cells before and after exposure to virus seemed to have little effect on either plaque size or viral titer.
The growth curves of LLC-MK2, Vero cell cultures and serological surveys of the virus were also described.抄録全体を表示PDF形式でダウンロード (3371K) -
II. Multiplication of Japanese Encephalitis Virus in the Mosquito Cell and Simian Cell CulturesJian-Yi Hsue1976 年 28 巻 Supplement 号 p. S87-S113
発行日: 1976/12/20
公開日: 2013/02/19
ジャーナル フリーThe growth and development of JE virus in established Culex pipiens var. molestus cell and Vero cell lines were described. Both of the two cell lines produced a comparable virus yield of about 7.0 log10 units PFU/ml, accompanied by definite cytopathic effects.
The mosquito cell line used in this study was found to set up a persistent infection, whereas the Vero cell line showed a short term cytocidal infection. Virus infected mosquito cell culture was serially subcultured 15 times over 470 days, and it was noted that the size of the plaque became smaller as the passage continued. A gradual loss in viral yield was observed.
Specific immunofluorescent reaction could be observed in both kinds of infected cells. A prominent increase of acid phosphatase activity was found in both of the infected cells. The specific immunofluorescent finding and positive acid phosphatase reaction were constantly observed prior to the appearance and the progression of the cytopathic effect in both of the cells.
The findings with the light microscope and the fluorescent methods suggest that the initial viral replication occurs in the perinuclear region of the cytoplasm.抄録全体を表示PDF形式でダウンロード (10498K) -
Shiu-chi Huang1976 年 28 巻 Supplement 号 p. S114-S156
発行日: 1976/12/20
公開日: 2013/02/19
ジャーナル フリーThe growth and development of dengue-4 virus in mice and Vero cells were studied morphologically by electron microscopy, fluorescent antibody staining, and acid phosphatase reaction, and the following results were obtained.
Before the manifestation of virus reproduction, pronounced acid phosphatase activity of host cells was observed. At this stage, specific fluorescence against dengue-4 virus appeared in the perinuclear region of infected Vero cells and gradually increased. By electron microscopy, virus particles were first found in the cytoplasm or in the endoplasmic reticula after a latent period of 18 hours, and their number increased successively. The virus particles then assembled in the vesicles or intracellular space, usually in random arrangement. In the late period of infection, cells became degenerated and massively arranged virus particles were found more easily both intra- and intercellularly. These findings corresponded well with the results of infectivity assay, the cytopathic effects observed, and the paralysis and death of mice. A budding process through cellular membrane structures for dengue-4 virus maturation was suggested.
The dengue-4 virus particles were found to be spherical in shape and estimated to be approximately 45-50 nm in diameter. Each was composed of an outer membrane of 4 nm thickness and an electrondense core,25 nm in diameter. Incomplete particles were not observed. Immature virus particles were also observed 24 hours after inoculation.抄録全体を表示PDF形式でダウンロード (37306K)
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