The effect of 3,3',5'-triiodothyronine (rT3) on angiotensin II (AII)-induced calcium mobilization and formation of inositol 1,4,5-trisphosphate (IP3) in vascular smooth muscle cells (VSMC) was studied in this paper. rT3 at concentrations of 10 and 50,μM inhibited All (1μM)-induced increase in cytosolic calcium concentration ([Ca2+]i) and IP3 formation in VSMC dose-dependently. These results indicate the possibility that rT3 which is regarded as an inactive metabolite of thyroxine (T4) has inhibitory effects on vascular smooth muscle function.
Primary cultures of murine cortical neurons and glia cells obtained from mouse embryos at 15 days of gestation were employed to study the polarized sorting of infected Herpes simplex virus type I (HSV-1)envelope glycoproteins. Following trypsinization the cortical neurons were successfully maintained at low density on polylysine-coated coverslips or culture dishes in a glucose-enriched medium. The intracellular distribution of viral glycoproteins was monitored by laser scanning confocal immunofluorescence microscopy. In mature, fully polarized neurons the HSV-1 glycoprotein moved from the perinuclear Golgilike region to the dendritic domains, whereas in underdeveloped immature neurons and glia cells no polarized distribution of the viral glycoproteins was observed. Since the HSV-1 glycoproteins are sorted to the basolateral plasma membrane of MDCK cells in a polarized manner', it is suggested that a common mechanism for polarized protein sorting could be shared between neurons and epithelial cells. Implications of the polarized sorting of the viral glycoproteins are also discussed on the basis for the pathogenesis of HSV-1 encephalitis.
A prominent, gonialangle, so called benign masseteric hypertrophy, is rather common and considered to be unattractive feature in the Orient. A face with this kind of deformity can only be corrected surgically Though most of the procedures are performed intraorally, exposure of the operation site is often not large enough for osteoplasty to proceed safely. Since the definite indication of the site of osteotomy is needed, especially in a narrower operative field, a special instrument was deviced to perform safe and accurate mandibular angle osteotomy.