At fertilization, mammalian eggs show repetitive transient [Ca
2+]
i rises each of which is due to Ca
2+ release from the endoplasmic reticulum through inositol 1,4,5-trisphosphate (IP
3) receptors. During fertilization, a factor from the sperm, the sperm factor, is released into the oocyte and induces a long-lasting series of Ca
2+ spikes (Ca
2+ oscillations) that are required for egg activation. IP3-producing enzyme phospholipase C zeta (PLCζ) is a strong candidate for the sperm factor. The Ca
2+ spikes initiate the extrusion of cortical granules that block the entry of other sperm. At the same time, maturation (M-phase) promoting factor (MPF) is inactivated by the Ca
2+ oscillations, resulting in exit from metaphase II arrest. Meiosis resumes with formation of the second polar body and complete meiotic division, one-cell embryos with the male and female pronuclei attain the first cleavage division through nuclear envelope breakdown.
View full abstract