The effect of a extremely low frequency magnetic field (ELFMF) on living biological cells was investigated by using a highly sensitive new mutagenesis method. A bacterial gene expression system for mutation repairing (
umu system) was employed for the sensitive evaluation.
Salmonella typhimurium TA1535 (pSK1002), a mutagen-sensitive bacterium that possesses a gene expression system with an SOS response to mutagenesis (
umu operon), fused with the β-galactosidase gene, was exposed to an ELFMF in a specially designed magnetic field loading chamber. The experimental results showed that the umu assay could be effectively applied to the evaluation of damage in DNA molecules by exposure to an ELFMF. However, no significant differences were observed in the umu gene expression intensity under experimental condition.
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