日本医科大学雑誌
Online ISSN : 1884-0108
Print ISSN : 0048-0444
ISSN-L : 0048-0444
62 巻, 4 号
選択された号の論文の12件中1~12を表示しています
  • 松倉 則夫, 恩田 昌彦, 山下 精彦
    1995 年 62 巻 4 号 p. 313-319
    発行日: 1995/07/15
    公開日: 2010/03/01
    ジャーナル フリー
  • In vitroにおける検討
    森川 哲行, 渋谷 昌彦, 酒井 茂利, 工藤 翔二
    1995 年 62 巻 4 号 p. 320-328
    発行日: 1995/07/15
    公開日: 2010/03/01
    ジャーナル フリー
    The effect of anticancer drugs on invasive capacity of Lu 135 human small-cell lung cancer cells was studied in vitro. The invasive capacity decreased in a dose-dependent manner when tumor cells were treated with the anticancer drugs cisplatin and etopside. The inhibition of tumor cell invasion was almost parallel with the inhibition of tumor cell growth. The anticancer drugs also suppressed tumor cell migration and the activity of the matrixdegrading protease, type IV collagenase, activity. But they did not suppress adhesion to basement membrane protain such as laminin, fibronectin, or type IV collagen.
    Because tumor cells express adhesion molecules on the cell surface, the effect of the anticancer drugs on the expression of one such molecule, integrin, was also studied. Lu 135 cells expressed α41 integrin on the cell surface, and the pattern of integrin expression did not change with exposure with the anticancer drugs.
    These data suggest that the anticancer drugs inhibit the invasive capacity by suppression of migration and type IV collagenase activity of tumor cells, and that they do not modulate adhesion to the extracellular matrix or cell surface adhesion molecules such as integrin.
    Furthermore, these findings indicate that anticancer drugs may be useful for anti-metastatic therapy in patients with small-cell lung cancer.
  • 深井 靖雄, 藤田 安一郎
    1995 年 62 巻 4 号 p. 329-338
    発行日: 1995/07/15
    公開日: 2010/03/01
    ジャーナル フリー
    1. Effects of afferent nerve stimulation on the waveform of the IS-spike were studied intracellularly in 101 motoneurons of cats which were either anesthetized with sodium pentobarbitone or decerebrated at the intercollicular level.
    2. In 78 motoneurons repetitive or double shock stimulation of afferent nerves (the L 7 or S 1 dorsal root, biceps-semitendinosus nerve and tibial nerve) could produce 2-4 kinds of IS-spikes, as distinguished by differences in waveform. In 34 motoneurons 2-3 small potentials were superimposed on the smallest IS-spike with progressively longer latencies to form a larger IS-spike. These potentials were thought as dendritic in origin because only the dendrites are known to be capable of exhibiting multiple spikes and in 14 motoneurons dendritic spikes were actually shown to exist.
    3. In 13 motoneurons following a repetitive stimulation of a dorsal root there occurred an increase in excitability of the membrane generating the IS-spike (the IS-membrane) in the absence of any appreciable changes in the resting membrane potential. This would indicate that the IS-membrane is subject to plastic changes or the IS-spike itself also originates in dendrites which are known to exhibit such changes.
  • 斎藤 仁子
    1995 年 62 巻 4 号 p. 339-350
    発行日: 1995/07/15
    公開日: 2010/03/01
    ジャーナル フリー
    The etiology of diabetic cataract is usually explained by the following process; the conversion of glucose and galactose to polyol by aldose reductase, then the accumulation of polyol in lens, and the opacity of lens. Another explanation is that the hyperoxidation of lens membrane due to an increase of active oxigen and lipid peroxide in lens induces diabetic cataract. The experimental animals used in the present study were rats with galactose cataract and streptozotocin cataract. We measured the levels of antioxidants (glutathione, ascorbic acid) and lipid peroxide (malonodialdehyde) in lens, aqueous humor and vitreous body. Furthermore we studied the effects of aldose reductase inhibitor (TAT) on these levels. In streptozotocin diabetes rats, the increased malonodialdehyde levels in lens, aqueous humor and serum were suppressed by TAT administration. In galactose and streptozotocin diabetes rats, the decreased levels of glutathione and ascorbic acid were suppressed by TAT administration.
  • 山本 達
    1995 年 62 巻 4 号 p. 351-359
    発行日: 1995/07/15
    公開日: 2010/03/01
    ジャーナル フリー
    The effect of argatroban, an anti-thrombin agent, on a 1.5×6.0 cm caudally-based flap raised on the backs of Wistar female rats was studied. Argatroban was administered to one group via an implanted osmotic pump, and nothing was administered to a control group.
    In experiment 1, the effect was evaluated by survival length (ratio of length to width) and a statistically significant difference (p<0.01) was found between the argatroban group and the control group.
    In experiment 2, at 3 spots along the midline (M 1, M 2, M 3) in each group, ISO2, (a parameter of oxygen saturation) and IHb (a parameter of hemogrobin concentration) were measured with a reflectance spectrophotometer, and FLOW (a parameter of blood flow) and MASS (a parameter of blood mass) were measured with a laser Doppler flowmeter. M 1 was a distal spot of flap, M 2 was a proximal spot on the flap and M 3 was a spot beside the flap used as a control. At M 1, flaps in both groups became necrotic. At M 2, flaps in the argatroban group survived, whereas those in the control group became necrotic, and each parameter indicated improvement in blood circulation only in the argatroban group. Thus, argatroban, which antagonizes thrombin, can improve microvascular circulation in congestive tissue and consequently help the flap survive.
    Argatroban has advantages over other agents that have been given to increase the length of the surviving experimented flap. In comparison with heparin, the coagulation time is easier to control. Also argatroban does not dilate peripheral vessels, which may be a demerit of alprostadil. Argatroban also promotes the reaction of plasminogen activator. The serum concentration of argatroban in this study was almost equal to that used clinically. Therefore, argatroban may be useful when elevating a flap clinically.
  • 赤石 文洋
    1995 年 62 巻 4 号 p. 360-368
    発行日: 1995/07/15
    公開日: 2010/03/01
    ジャーナル フリー
    The purpose of this study is to investigate the biomechanical properties of the anterior longitudinal ligament (ALL) and the posterior longitudinal ligament (PLL) of the human cervical spine. Twenty C 3-C 4 and twenty C 5-C 6 motion segments are dissected from the spines of human cadavers. Experimental specimens of the bone-ligament-bone complex are prepared for mounting on a testing jig. Static tensile tests are performed with an Instron-type material test system. Dynamic biomechanical properties were tested with a biomechanical spectrometer.
    The failure load and failure stress of the PLL tend to be higher than those of the ALL, but there are no statistically significant differences between them. The tan 6 of the ALL is higher than that of the PLL (p<0.01). The dynamic stiffness of the PLL is higher than that of the ALL (p<0.01), but there was no statistically significant difference in the dynamic elastic modulus between the ALL and the PLL.
    These results imply that the PLL has better biomechanical properties as a stabilizer for cervical motion than the ALL in the lower cervical spine.
  • 清水 要吉
    1995 年 62 巻 4 号 p. 369-376
    発行日: 1995/07/15
    公開日: 2010/03/01
    ジャーナル フリー
    This study involved a clinical evaluation and radiological follow-up of patients who underwent laminoplasty by longitudinal splitting of the spinous process for compressive myelopathy.
    The subjects were 47 patients with myelopathy caused by cervical spondylosis or ossification of the posterior longitudinal ligament in the cervical spine. The average age at the time of surgery was 61, and the average follow-up period was 3 years.
    Overall results: the preoperative score, by the Japanese Orthopaedic Association scoring system, was 9.8, and the postoperative score was 13.9; the average recovery rate was 54.3%.
    Lateral roentgenograms showed a decreased lordosis in the cervical curvature in 50% of the patients after laminoplasty, especially in those patients where stripping of the attachments of the semispinalis cervicis to the spinous process of C2 occurred.
    The recovery rate was good in patients with postoperative lordortic or strainghtened necks (61.1% and 55.0%, respectively), but it was poor in those with kyphotic or S-shaped necks (average: 36.1%).
    The postoperative range of motion of the cervical spine had decreased to 43% of the preoperative range.
  • 組織学的所見および水分動態との関連
    小林 薫
    1995 年 62 巻 4 号 p. 377-385
    発行日: 1995/07/15
    公開日: 2010/03/01
    ジャーナル フリー
    The purpose of this study is to investigate the visco-elasticity, histology and hydrodynamics of transplanted menisci following transplantation. Experiments to examine visco-elasticity are performed on 30 rabbits, to examine NMR on 20 rabbits and on 12 rabbits respectively for histological study. Under intravenous general anesthesia the medial meniscus was completely resected, and a cryopreserved allograft meniscus was implanted. The rabbits are sacrificed 1, 2, 3 and 5 months post-operatively, and the implanted menisci are removed for examination.
    One month after surgery, dynamic stiffness is markedly lower in the implanted menisci than in the controls. Thereafter, it gradually increased. Three months after surgery, there is no significant difference between the transplanted menisci and the controls. However the value of tanδ which serves a indicator of dynamic visco-elasticity is larger in the implanted menisci than in the control specimens throughout the experiment.
    The water content was higher in the transplanted menisci throughout. The 1H-NMR relaxation time (T2) was markedly prolonged 1 month post-operatively, but shortened rapidly after that. Nevertheless, it is still longer than in the control specimen after 5 months.
    In the histological study, no chondrocytes are observed in the transplanted menisci, and the matrix was poorly stained with Eosin 1 month after surgery. Thereafter, granulomatous tissues proliferated in the menisci, and by 5 months after surgery, the chondrocytes appear to be almost normal. However, matrix was weakly stained with Eosin.
    These results suggest that the interactions between high molecular weight matrix and water in transplanted menisci are different from those in normal menisci, and that therefore the viscosity of transplanted menisci is higher.
  • Yoshihiro Fukuo, Mikio Nagashima, Naoko Soya-Ohmura, Shinichi Takagi, ...
    1995 年 62 巻 4 号 p. 386-390
    発行日: 1995/07/15
    公開日: 2010/03/01
    ジャーナル フリー
    It has been reported that the HMG-CoA reductase inhibitor simvastatin does not always effectively lower plasma LDL. This drug acts to monocytes/macrophages directly and inhibits cholesterol ester accumulation in these cells. However cytokine production in macrophages when simvastatin was administrated has not been described. In this study, we examined whether simvastatin affects cytokine production in human monocyte-derived macrophages. Simvastatin at doses ranging from 10-9 to 10-5 M did not affect the synthesis of proinflammatory cytokines (IL-1β, IL-6, IL-8) from human peripheral mononuclear cells. In addition, any changes in cytokine-induced cytokine production (IL-1-induced IL-8 synthesis) were not detected after the addition of simvastatin. The present results suggest that simvastatin suppresses foam cell formation in monocyte/macrophage, without affecting the immunological or inflammatory functions of these cells.
  • 孫 〓, 塩谷 猛, 王 若皎, 石渡 俊行, 内藤 善哉, 喬 炎, 浅野 伍朗, 大秋 美治, 国見 聰宏, 武内 務, 土居 大祐, ...
    1995 年 62 巻 4 号 p. 391-395
    発行日: 1995/07/15
    公開日: 2010/03/01
    ジャーナル フリー
    Two autopsy cases (69 and 87-year-old women) of malignant mixed müllerian tumor (MMMT) following radiation therapy for uterine cervical cancer sixteen and twenty years ago respectively were reported. They were admitted due to abdominal pain anddiagnosed as ileus. In the first case, CT examination revealed a tumor measuring about 8 ×10cm in size in the uterine posterior wall. Recurrence of the uterine cancer was suspected and hysterooophorectomy combined with sigmoidectomy was performed. In the second case, artificial anus formation was performed because of sigmoid stricture by the invasion ofthe tumor. Histologically, tumors in both cases were composed of carcinomatous and sarcomatous components including heterologous elements such as cartilage. The patients died of extensive spreads and metastasis in the liver and alimentary tract.
  • 最近の薬物療法がRAの自然経過に及ぼす影響と日常診療における治療目標
    藤森 十郎, 吉野 槇一
    1995 年 62 巻 4 号 p. 396-400
    発行日: 1995/07/15
    公開日: 2010/03/01
    ジャーナル フリー
  • HPVと結核菌について
    川並 汪一, 尾見 徳弥, 荻原 英子
    1995 年 62 巻 4 号 p. 401-405
    発行日: 1995/07/15
    公開日: 2010/03/01
    ジャーナル フリー
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