Astrocyte (AS), a kind of glial cell, is thought to play an important role in information processing in the brain. AS has metabotropic glutamate receptors, and the application of glutamate elicits the spatio-temporal pattern of Ca2+. AS also shows spontaneous Ca2+ concentration ([Ca2+]i) oscillation. The mechanisms of these phenomena have not yet been elucidated. In the present study, we proposed a Phospholipase C (PLC)δ model that could induce several spatio-temporal patterns. PLCδ is an intracellular enzyme whose concentration is controlled by the transcriptional process. These results suggest that the spatio-temporal pattern of AS [Ca2+]i is modulated by PLC δ.
This paper reports on neural recording chips with multi-recording microprobes for use in the study of nervous system. Penetrating microprobes array has been fabricated at predetermined sites on a Si substrate by selective vapor-liquid-solid (VLS) Si growth. Diameter, position and length of the Si probes were controlled. Typical Si microprobe dimensions in this work include a length of 60µm and a diameter of 2µm with dimensions and distribution similar to the neurons in a tissue, low neural invasiveness and low tissue damages. In addition, the Si microprobes were fabricated on an integrated circuit (IC). In neuroscience, the Si microprobe electrode array chip will be an effective technique for providing accurate information from neurons in tissue.