巻号一覧
23 巻, 6 号
選択された号の論文の9件中1～9を表示しています
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Shirley A. HOLTSCLAW, Charles E. CLARK1977 年 23 巻 6 号 p. 475-480
発行日: 1977年
公開日: 2009/04/28
ジャーナル フリーAscorbic acid in concentrations of 100μg/ml of culture medium protects HeLa cells against the protein synthesis inhibition activi-ty of ricin, a phytotoxin isolated from castor beans.抄録全体を表示PDF形式でダウンロード (693K) -
Mary L. DUKE, Constance KIES, Hazel M. FOX1977 年 23 巻 6 号 p. 481-489
発行日: 1977年
公開日: 2009/04/28
ジャーナル フリーThe addition of a vitamin to a diet of humans has been shown to increase the excretion of that vitamin. The effects of an increase of one vitamin on another have not been investigated. The objective of the current project was to compare the effects of two supplementation patterns on the niacin and pantothenic acid excretion values of humans consuming a peanut butter-based diet. Two groups each received one of two supplementation regimens. One group received niacin, a multi-vitamin, or no supplement. One group received methionine alone, pantothenic acid alone or methionine plus pantothenic acid. The addition of either vitamin resulted in increased excretion of that vitamin. Urinary niacin excretion of the group that received pantothenic acid and/or methionine was greater than that observed with a multi-vitamin or no supplement. Urinary pantothenic acid excretion was suppressed when niacin was a supplement. Urinary pantothenic acid excretion of the methionine supplement group was greater than the excretion of the groups which received either niacin or multi-vitamin supplements. These data suggest some possible dangers in indiscriminate supplemen-tation of food products.抄録全体を表示PDF形式でダウンロード (1008K) -
矢野 秀雄, 川島 良治1977 年 23 巻 6 号 p. 491-496
発行日: 1977年
公開日: 2009/04/28
ジャーナル フリーThe object of the present experiment is to determine the effect of thiamine administration on lactic acid concentration in blood plasma and the mineral metabolism in sheep given low and high con-centrate rations.
A high concentrate ration containing 90% concentrate and 10% roughage was fed to one group of sheep and a low concentrate ration containing 60% concentrate was given to the other group. Thiamine was intra-muscularly injected into every sheep at a level of 50mg of thiamine tetrahydrofurfuryl disulfide per day.
No great difference was found in the plasma lactic acid concentration between the animals fed the low and high concentrate rations, and those between the control and thiamine injection periods. Thiamine injection appeared to increase urine and fecal magnesium excretion and significantly decreased magnesium balance (p<0.05). It may be conceivable that endogenous magnesium excretion is enhanced by thiamine administra-tion in sheep.抄録全体を表示PDF形式でダウンロード (619K) -
森内 幸子, 吉沢 節子, 細谷 憲政1977 年 23 巻 6 号 p. 497-504
発行日: 1977年
公開日: 2009/06/15
ジャーナル フリーPolyacrylamide disc gel electrophoresis of n-butanol solubilized alkaline phosphatase from chick duodenum revealed that the change of alkaline phosphatase induced by 1, 25-(OH)2D3 involved the transformation of desialoenzyme to sialoenzyme. The initial stimula-tion by 1, 25-(OH)2D3 of the incorporation of sialic acid into duodenal microsomes corresponded with the initial increase in calcium absorption. After this initial stimulation, there was a rapid decline in sialic acid incorporation into microsomes decreasing below control levels at 24hr. Calcium concentration in the microsomes followed a pattern similar to the incorporation of sialic acid into microsomes. The depressed sialic acid incorporation was reversed by the addition of calcium in vitro.
These results suggest that the initial action of 1, 25-(OH)2D3 is to change the membrane permeability to calcium and to change the subcellular distribution of calcium in the small intestine. The accumulated calcium in the microsomes then stimulates the sialic acid incorporation into desialoenzyme. This results in the changes of isozyme pattern of alkaline phosphatase, viz, the transformation of desialoenzyme to sialoenzyme. The transformed alkaline phosphatase might be one of the factors in-volved more directly in the regulation of calcium transport in intestine.抄録全体を表示PDF形式でダウンロード (1349K) -
岡田 美津子, 岩見 玉子1977 年 23 巻 6 号 p. 505-512
発行日: 1977年
公開日: 2009/04/28
ジャーナル フリーHepatic cholesterol contents in rats fed a 70% or 20% casein diet with or without pyridoxine was determined. In the case of the 70% casein group, pyridoxine-deficient rats showed a higher content than the control. The increment was mainly due to the accumulation of an ester form of the cholesterol. On the other hand, pyridoxine-deficient rats in the 20% casein group showed a slightly lower content. The cholesterol content in liver microsomal fractions was lower in the 20%-casein pyridoxine-deficient group and serum cholesterol level was lower in the 70%-casein pyridoxine-deficient group than those in respective control groups. Incorporation of [14C]acetate into cholesterol was studied using liver slices, and significant stimulation was observed in pyridoxine-deficient rat fed a 20% or 70% casein diet. There was no difference in intestinal cholesterogenesis between the control and the deficient groups.抄録全体を表示PDF形式でダウンロード (989K) -
康村 満枝, 岡野 登志夫, 水野 久美子, 小林 正1977 年 23 巻 6 号 p. 513-523
発行日: 1977年
公開日: 2009/04/28
ジャーナル フリーLipids were extracted from the skin of normal rats with chloroform-methanol (1:1) and the solvent evaporated. The resulting residue was saponified, and the unsaponifiable matter extracted with benzene was purified by subjecting the extract successively to hydroxy-alkoxypropyl (HAP) Sephadex column chromatography, silica-gel column chromatography and preparative thin-layer chromatography (TLC). The resulting purified provitamin D fraction was confirmed to contain 7-dehydrocholesterol (7-DHC) based on data of ultraviolet (UV) spectrum, high-performance liquid chromatography (HPLC), gas-liquid chromatography (GLC) and gas-liquid chromatography-mass spectro-metry (GC-MS). Based on the results of GC-MS and mass chromato-graphy, it was also confirmed that the fraction contained small amounts of cholesterol, lathosterol and two other unknown sterols besides 7-DHC. Then, the determination of 7-DHC in rat skin was investigated and a HPLC method was proposed. This method included extraction of lipids from small pieces of rat skin with chloroform-methanol (1:1), saponifica-tion, isolation of the unsaponifiable matter and application to HPLC. The estimated average values of 7-DHC in normal rat skin (20 weeks old), with and without UV irradiation, were 107.7 and 103.8μg/g, respectively. These were significantly higher than the corresponding values of vitamin D-deficient rat skin. UV irradiation of rat skin did not affect the change of 7-DHC content in the tissue.抄録全体を表示PDF形式でダウンロード (1294K) -
浜口 陽一, 八木 典子, 西野 明子, 望月 てる代, 水上 戴子, 三好 正満1977 年 23 巻 6 号 p. 525-534
発行日: 1977年
公開日: 2009/04/28
ジャーナル フリーA lethal protein with hemagglutinating activity but with-out trypsin inhibitory activity was isolated from beans of Phaseolus vulgaris, cultiva, and Kintoki and proved homogeneous by ultracentri-fugation, disc polyacrylamide gel electrophoresis, sodium dodesyl sulfate polyacrylamide gel electrophoresis and isoelectric focusing. The mole cular weight was estimated to be 104, 000 by ultracentrifugal analysis and gel filtration on Sephadex G-200. The molecule dissociates into three identical subunits in the presence of 8M urea or 0.1% sodium dodesyl sulfate. The amino acid composition was characterized by the high content of aspartic acid and the complete absence of methionine and cystine. The carbohydrate content was 8.1%; 5.0% mannose and 3.1% glucosamine. The addition of the lethal protein to a basal diet (0.4%) resulted in the intensive depression of the growth and finally in the death of rats. The intraperitoneal injection of 250μg per g body weight of mouse brought about an acute toxicity which caused death of all the injected mice.抄録全体を表示PDF形式でダウンロード (1752K) -
中川 一郎, 佐々木 敦子1977 年 23 巻 6 号 p. 535-548
発行日: 1977年
公開日: 2009/04/28
ジャーナル フリーThree experiments were carried out on a total of 78 female rats to examine the effect of an excess intake of leucine, with and without additions of vitamin B6 and/or niacin, on the excretion of tryptophan and niacin metabolites, and on branched-chain amino acid transaminase activity in some organs and on serum amino acid levels. An excess in-take of leucine (10% leucine) retarded the growth of rats, and vitamin B6 and niacin deficiencies did not enhance this effect. Urinary excretion of N-methyl-2-pyridone-5-carboxamide (pyridone) and niacin was scarcely affected by the excess intake of leucine, but the excretion of N'-methylnicotinamide (N-MNA) seemed to decrease. Urinary excretion of quinolinic acid decreased during the experimental period in all the rats. These facts suggest that the conversion of quinolinic acid to nicotinamide adenine dinucleotide phosphate (NADP) may not be in-hibited by an excess intake of leucine. Urinary excretion of 5-hydroxy-indoleacetic acid increased in all the rats, and there was no significant difference among them. Branched-chain amino acid transaminase activity decreased in vitamin B6 deficiency, but rather increased in excess leucine.
The serum leucine level did not increase in humans in our previous experiment, but in the present experiment in rats, it increased on the 17th and the 21st days of administration of 10% leucine. Serum valine and isoleucine levels decreased. On the contrary, serum tryptophan level increased.
An excess intake of leucine, even with a large amount of it, did not seem to be related to the incidence of pellagra, so far as the urinary excretions of tryptophan and niacin metabolites are concerned.抄録全体を表示PDF形式でダウンロード (1473K) -
亀田 謙介, 岡安 多香子, 小野 輝夫, 今井 陽1977 年 23 巻 6 号 p. 549-552
発行日: 1977年
公開日: 2009/04/28
ジャーナル フリーPDF形式でダウンロード (391K)
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