巻号一覧
25 巻, 4 号
選択された号の論文の10件中1～10を表示しています
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岡村 正人1979 年 25 巻 4 号 p. 269-279
発行日: 1979年
公開日: 2009/04/28
ジャーナル フリーUptake of AsA and DAsA by human cells, i.e., erythrocytes and HeLa cells, was examined in vitro. AsA was taken up very slowly, but DAsA was taken up very rapidly by erythrocytes to establish equilibrium after 1 minute. Uptake of the vitamins by HeLa cells was similar to that by erythrocytes, except there was an uptake of DAsA that reached saturation after 5 minutes. The DAsA taken up was reduced in part to AsA and the concentrations of DAsA inside and outside the cells became almost equal. GSH was responsible for this reduction. Although DAsA was evidently a more permeant form than AsA in the case of human cells, the relevance of this to the uptake of vitamin C by the tissues in viva remains uncertain.抄録全体を表示PDF形式でダウンロード (902K) -
八木 典子, 糸川 嘉則1979 年 25 巻 4 号 p. 281-287
発行日: 1979年
公開日: 2009/04/28
ジャーナル フリーWe have clarified that thiamine is cleaved by residual chlorine in proportion to the rise in temperature, pH and concentration of residual chlorine. When rice was boiled in an electric rice cooker, the thiamine in the rice was cleaved by residual chlorine. It is assumed that thiamine is cleaved into hydroxymethylpyrimidine and the thiazole moiety.抄録全体を表示PDF形式でダウンロード (516K) -
笠井 佐夫, 山中 成子, 王 秀娟, 松井 邦夫1979 年 25 巻 4 号 p. 289-298
発行日: 1979年
公開日: 2009/04/28
ジャーナル フリーTwo new 8-O-alkyl derivatives of riboflavin (RF), i.e., 8-methoxy- (MOF), and 8-ethoxy-8-demethyl-D-riboflavin (EOF), their tetraacetate, and the tetraacetate of 8-hydroxy-8-demethyl-D-riboflavin (HOF) were synthesized. The anti-RF activity of MOF, EOF and HOF was estimated from the ratio CR/CI, where CI is the concentration of test flavin added to the culture medium and CR is the minimum concentration of RF needed to restore the growth inhibition. Their activity was also compared with that of roseoflavin (RoF). The decreasing order of anti-RF activity was as follows: MOF>RoF>EOF in Sarcina lutea; RoF>MOF>EOF in Bacillus cereus and Staphylococcus aureus. HOF showed no activity in any of the bacteria tested. The redox potential of these compounds decreases as follows: RF>RoF>EOF>MOF>HOF, and the anti-RF activity of MOF and EOF could be explained by the redox potential difference between these compounds and RF.抄録全体を表示PDF形式でダウンロード (958K) -
重岡 成, 中野 長久, 北岡 正三郎1979 年 25 巻 4 号 p. 299-307
発行日: 1979年
公開日: 2009/04/28
ジャーナル フリーD-Glucose and D-galactose were the starting materials of L-ascorbic acid biosynthesis in Euglena gracilis as evidenced by feeding experiments of unlabeled and labeled sugars, but D-glucose was the more effective precursor. The addition of various acid derivatives of D-glucose and D-galactose, with the exception of D-glucono-δ-lactone, considerably augmented L-ascorbic acid formation. D-Galacturonic acid and L-galactono-γ-lactone showed greater effects than did D-glucurono-γ-lactone and L-gulono-γ-lactone. The results of isotopic dilution experiments also showed the preference for the galacto-configuration. Fed U-14C-D-glucose was transformed into labeled D-galacturonic acid to a greater extent than into labeled D-glucuronic acid, and added D-galacturonic acid only caused extensive accumulation of labeled D-galacturonic acid. These results together show that the pathway involving D-galacturonic acid and L-galactono-γ-lactone is the major one, the one involving D-glucuronic acid L-gulono-γ-lactone being the minor one. A likely pathway for L-ascorbic acid biosynthesis in Euglena is proposed in the Scheme, which thus involves uronic acid intermediates and configurational inversion.抄録全体を表示PDF形式でダウンロード (951K) -
諏訪 純一, 小柳 達男, 木村 修一1979 年 25 巻 4 号 p. 309-315
発行日: 1979年
公開日: 2009/04/28
ジャーナル フリーThe goitrogenicities of various preparations obtained from soybean were studied using rats. Thyroid weight, 131I uptake and the ratio of 131I-monoiodotyrosine to 131I-diiodotyrosine of the gland in rats fed the preparation was measured to determine the activity of these preparations. It was resulted that the group of rats receiving the curd had the most hypertrophied thyroid, the enlargement being equal to that in the group of these receiving soybean in the 8 weeks' feeding experiment. No difference on the content of iodine were found with the various diet preparations. The proteolytic digestion of soybean curd did not eliminate the goitrogenic property, thus, the goitrogen seemes not to be a protein or peptide-like substance.抄録全体を表示PDF形式でダウンロード (901K) -
名武 昌人, 団野 源一, 前田 利和, 川村 和彦, 金沢 和樹1979 年 25 巻 4 号 p. 317-332
発行日: 1979年
公開日: 2009/04/28
ジャーナル フリーIt was confirmed by the procedure of rec-assay that DNA-damaging activities were formed in the reaction systems containing nitrite and phenol derivatives including BHA, tryptophan or cysteine under gastric pH conditions. The mutagenic action of the nitrite-BHA, nitrite-tryptophan and nitrite-cysteine systems was also tested according to Ames' method using Salmonella typhimurium TA 1535 and TA 98. The mutagenic activity was observed in the nitrite-tryptophan and nitrite-cysteine systems, though the nitrite-BHA system did not show the activity. The DNA-damaging products were generally labile, i.e., the activity decreased significantly after 1.5 to 2 hours of the reaction, except in the case of the nitrite-BHA system. The DNA-damaging activity in the nitrite-BHA system did not decrease even after 48 hours of the reaction. Nitrosophenol derivatives themselves showed the DNA-damaging activity at pH 1. The active product in the nitrite-BHA system was isolated and the structure was determined to be 2-tert-butyl-quinone. This compound gave a positive rec-assay test, and showed no mutagenesis by Ames' method. The active product from the nitrite-cysteine system was infered to be nitrosocysteine, and the product showed both DNA-damaging and mutagenic activity.抄録全体を表示PDF形式でダウンロード (2148K) -
稲垣 泰介, 片山 真之, 三浦 一夫1979 年 25 巻 4 号 p. 333-342
発行日: 1979年
公開日: 2009/04/28
ジャーナル フリーFour chromatographically different proteases were par-tially purified from defatted soybean flour, and their pH optima were around 5.0 to 5.6 using casein as the substrate. These soybean proteases were designated S1, S2, S3 and S4 according to their order of elution from a DEAE-cellulose column. Each gave a single peak of caseinolytic activity on a Sephadex G-200 column chromatogram, and corresponded to the molecular weights of about 50, 000(S1), 35, 000(S2), 60, 000(S3) and 200, 000(S4). The proteases could hydrolyze casein and poly-Glu. α-Casein was more rapidly hydrolyzed than β-casein, but the esters or dipeptide could not be hydrolyzed. Aliquots of 10-3 M Hg2+, Cu2+ and Zn2+ inhibited the caseinolytic activities by 70% to 90%, while other cations, Mn2+, Mg2+, Ca2+ and Ni2+, at the same concentration did not. SPI (10-5M) inhibited 80-90% of their activities, and EPNP (10-5M) inhibited their activities 30-60%, but DFP (10-3 M), SSI (10-3M), PCMB (10-4M), NEM (10-3M) and EDTA (10-3 M) were not inhibitory. The above results indicate that proteases S1, S2, S3 and S4 from defatted soybean flour can be classified as acid proteases.抄録全体を表示PDF形式でダウンロード (787K) -
伊藤 允好, 小玉 晶子, 村田 昌子, 小林 摩美, 月田 潔, 七田 芳則, 吉沢 透1979 年 25 巻 4 号 p. 343-345
発行日: 1979年
公開日: 2009/04/28
ジャーナル フリーPDF形式でダウンロード (278K) -
田寺 謙次郎, 中村 真保美, 八木 史郎, 小林 昭1979 年 25 巻 4 号 p. 347-350
発行日: 1979年
公開日: 2009/04/28
ジャーナル フリーPDF形式でダウンロード (359K) -
1979 年 25 巻 4 号 p. e1
発行日: 1979年
公開日: 2009/04/28
ジャーナル フリーPDF形式でダウンロード (20K)
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