Hematological studies on the chronic CCl
4 poisoning were performed in thirteen female rabbits, which were divided into five controls and eight CCl
4 injected group. The CCl
4 animals were administered with the poison three times a week by subcutaneous routes in the dose of 0.022 cc CCl
4 per kg. body weight which was determined by the previous study. Three of CCl
4 poisoning group were used for histological observation at about first month from the beginning of the experiment. The examinations were made approximately once a week concerning the following items; body weight, red blood cell and leulocyte count, hemoglobin content, hematocrit value, icteric index, color index, volume index, average diameter of erythrocytes, osmotic fragility of red cells, plasma volume, serum iron level, organ iron contents, weight of organs and microscopic examination of organ tissues. The results obtained were as follows: 1. The average body weight of controls increased gradually more than the CCl
4 group which kept on constant level along the course of the experiment. 2. Red cell count, hemoglobin content, leucocyte count and hematocrit value in CCl
4 group ran parallel with each other, and all of them showed two peaks throughout the course. 3. The icteric index of CCl
4 group was maintained on a higher level than that of controls. In the course of the experiment it was highest in the earlier stage of intoxication. 4. In the poisoned group the average diameter of red cells, color index and volume index were increased. 5. The osmotic fragility of red cells in the CCl
4 group showed a tendency to reduce after two weeks. 6. The plasma volume as well as the blood volume of CCl
4 group had a tendency to increase gradually. 7. The serum iron level in the CCl
4 group increased gradually and reached its highest level at the third week. 8. Evidence of changes in iron content of organs of the CCl
4 group was not found. 9. The liver of poisonned animals gained in weight in the beginning, but decreased at the end. The weights of the poisonned spleen and kindey were increased and that of the poisoned heart decreased. The most characteristic gain of weight, however, was found in the adrenal gland. 10. Concerning microscopic findings of organ tissues, the most marked change was found in the liver, which showed earlier features of cirrhosis during the first month of the poisoning and at the end of the experiment a progressed, definite liver cirrhosis was revealed. The bone marrow tissues were microscopically hypoplastic at the first month, but they became hyperplastic at the end.
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