In the mass examination of occupational diseases, erythrocyte counting is a method most often adopted to find out anemia. The conventional method for erythrocyte count with hemocytometer, however, has many weak point. It requires high skill and rather expensive equipments such as a microscope, hemocytometers, and so on. Besides, it requires a lot of time, which may otherwise be much reduced. In our present study, a comparative analysis of microhematocrit method, erythrocyte count, Wintrobe hematocrit method and Sahli's method for assessing hemoglobin was done as to their reliability, cost and efficiency, to find a one which is most suitable for mass examination. The reliability of these laboratory methods was checked by correlation coefficient, coefficient of variation, Spearman-Brown's formula, Rulon's formula, and by the Discrimination Index we introduced recently. The microhematocrit method was proved to be much more excellent than erythrocyte counting in its reliability by any of these means of checking. Among the four, the Wintrobe's method was found to be most reliable, which was followed by microhematorit method, Sahli's method, erythrocyte counting in the order of reliability. By the way, there was no significant difference of the reliability between microhematocrit method and Sahli's method checked by our Discrimination Index. Our present study showed that microhematocrit method is much more excellent than erythrocyte count not only as to reliability but also cost and efficiency. This encourages more frequent application of microhematocrit method in the mass examination.
View full abstract