岡山医学会雑誌
Online ISSN : 1882-4528
Print ISSN : 0030-1558
106 巻, 11-12 号
選択された号の論文の7件中1~7を表示しています
  • 石岡 一成
    1994 年 106 巻 11-12 号 p. 1105-1116
    発行日: 1994年
    公開日: 2009/03/30
    ジャーナル フリー
    Sodium pentobarbital has been used as a representative anesthetic in the experimental system using the canine excised cross-circulated heart preparation. In the present study, fentanyl was established as a new anesthetic in this experimental system. The mechanoenergetic effects of fentanyl on the excised heart were investigated by using the framework of Emax (the slope of the end-systolic pressure-volume relation), PVA (pressure-volume area) and VO2 (left ventricle oxygen consumption per beat). To determine the effects of fentanyl on the support dog, the systemic arterial blood pressure, heart rate, and plasma catecholamine concentration were measured.
    In the excised heart, fentanyl did not change the slope of the PVA-VO2 relation. This indicated that the cardiac mechanoenergetics was not qualitatively changed by fentanyl. In the support dog, fentanyl decreased the heart rate, but did not change the systemic arterial blood pressure.
    Fentanyl anesthesia is considered to be useful as an anesthetic for the experimental system using the canine excised cross-circulated heart preparation.
  • 佐牟田 健
    1994 年 106 巻 11-12 号 p. 1117-1124
    発行日: 1994年
    公開日: 2009/03/30
    ジャーナル フリー
    We investigated the variation in the protection of energy status by isoflurane in isolated hepatocytes with the isoflurane dose, duration of anoxia, and reoxygenation by measurements of ATP, ADP, and AMP, in the cells. During 30 min of anoxia, in the presence of 1.4, 2.8, 4.2% isoflurane the ATP concentration was more than that with 0% isoflurane. With a 30-35 min incubation in the presence of 1.4% isoflurane, there was a modest decrease in energy charge during anoxia, partially prevented by isoflurane and completely reversed by reoxygenation, and no decrease in the total adenine nucleotide. With a 90-120 min incubation, isoflurane partly prevented the decreases in both energy charge and total adenine nucleotide during both anoxia and reoxygenation. We conclude that at doses in the clinical range, isoflurane partially protected isolated hepatocytes against decreases in both energy charge and total adenine nucleotide occurring either during short or long anoxia.
  • 由井 治郎
    1994 年 106 巻 11-12 号 p. 1125-1142
    発行日: 1994年
    公開日: 2009/03/30
    ジャーナル フリー
    Using tumor-bearing C3H/He mice, in which syngeneic MH-134 tumor cells were transplanted into its back subcutaneously, the possibility of drug-induced splenectomy using cyclophosphamide (CY) and indomethacin (INDO), the timing of chemo- and immuno-therapy, and the usefulness of the combination of surgical splenectomy with immunotherapy were investigated. In patients with gastric cancer, the significance of splenectomy and its combination with immunochemotherapy was also studied.
    The administration of CY in mice on day 3 after tumor transplantation in the initial stage of the tumor prolonged the survival time, while INDO under the same condition shortened it only when administered on day 3 after transplantation. The administration of an immunopotentiator, OK-432, prior to CY administration shortened the survival time, compared with that of single administration of CY, whereas the OK-432 administration on day 2 after the administration of CY prolonged the survival time. In the immunochemotherapy in which CY was administered on day 7 after transplantation of tumor and OK-432 was given every two days after additional 2 days, the anti-tumor effect of drug-induced splenectomy was not observed as judged from the tumor proliferating curve, but the splenectomy on day 3 after tumor transplantation, in the initial stage of the tumor, prolonged the survival time. Similar results were observed, when INDO was used instead of CY.
    The concomitant splenectomy in the initial stage of gastric cancer resulted in a more effective prolongation of survival time. In the cases with adjuvant immunochemotherapy, preservation of the spleen in advanced cancer and splenectomy in the terminal stage of cancer were found to be preferable for effective prolongation of the survival time.
  • 入江 正一郎
    1994 年 106 巻 11-12 号 p. 1143-1157
    発行日: 1994年
    公開日: 2009/03/30
    ジャーナル フリー
    DPB has been known as a chronic respiratory infection with poor prognosis until introduction of erythromycin (EM) therapy with low-dose and long-term administration. As symptoms and prognosis of DPB were improved enormously under EM therapy, EM therapy on DPB was analyzed to clarify the action and mechanisms.
    EM in a daily dose of 600 mg was administered to 9 patients with DPB for about 38 months and 7 normal volunteers for 2 months. Most patients with DPB showed the relief of symptoms within 2 months, although the EM concentrations in the blood and sputa were lower than the antibacterial therapeutic level. Immunological examination revealed the decrease in cold agglutinin titer and CD4/CD8 ratio after EM therapy. The neutrophil chemotactic factor (NCF) derived from the mononuclear cell culture of DPB patients was higher than that in the normal control. EM therapy decreased the level of NCF not only in patients with DPB but also in normal volunteers.
    These findings indicate that the immunological effects of EM play an important role in the treatment of DPB.
  • 光学顕微鏡的および電子顕微鏡的研究
    木村 康裕
    1994 年 106 巻 11-12 号 p. 1159-1170
    発行日: 1994年
    公開日: 2009/03/30
    ジャーナル フリー
    Horseradish peroxidase (HRP) was applied to the rat sciatic nerve and the uptake, retrograde transport and interneuronal fate of HRP were examined by light and electron microscopy. Furthermore, FeCl3 which is very toxic to the nervous system was also applied into the rat cerebral sensory motor cortex and examined with Perls iron staining and Perls + DAB high sensitive iron staining methods for light microscopy. After the injection of iron into the rat sensory-motor cortex, the ultrastructual morphological effects on the neurons and glia cells in the hippocampus were also examined by electron microscopy.
    The HRP labeled neurons of the spinal ganglia and spinal anterior motor neurons were observed within 12 hours after application of HRP into the sciatic nerve. The HRP positive brown granules were accumulated in the perinuclear regions. Ultrastructurally, these granules appeared to be multivesicular and lysosomal bodies in the perinuclear regions. These granules were degraded by the lysosomal system disappearing 2 weeks after the injection.
    In the cases of FeCl3 injection, many neurons which existed in the vicinity of the injected area also showed iron uptake. These neurons contained abundant small brown materials in the cytoplasm. Ultrastructurally, the pyramidal neurons showed the degenerated changes, the nuclei were pyknotic and the cytoplasmatic materials were amorphous. In the neurons which showed iron uptake, a cause of the epileptic abnormal discharge, there were no degenerative findings. These neurons probably showed the abnormal excitation and seizure discharge and induced the epileptic seizures in the iron-induced epileptic rats models.
  • 矢尾 和久, 篠沢 真哉, 五味田 裕
    1994 年 106 巻 11-12 号 p. 1171-1175
    発行日: 1994年
    公開日: 2009/03/30
    ジャーナル フリー
    Adriamycin (ADM), an anthracycline antibiotic, has shown marked activity against a wide range of human neoplasms, but its clinical use has been limited because of the risk of dose-dependent severe cardiotoxicity. The tissue distribution of ADM was studied by highperformance liquid chromatography in comparison to those of anthracycline analogues, 4'-o-tetrahydropyranyl adriamycin (THP) and 4'-epi adriamycin (4'-epi) synthesized to decrease the toxicity. The concentration of unmetabolized ADM in the heart was higher than those of THP and 4'-epi 24hr after administration; namely, the concentrations of THP and 4'-epi were one-fourth and one-fifth of that of ADM, respectively. The concentrations of the metabolized THP and 4'-epi (aglycones) showed low values 12hr after administration, being about one-fourth or one-eighth of that of ADM. The concentrations of these unmetabolized drugs in the liver and kidney were in the decreasing order of 4'-epi, ADM and THP and that of the aglycones was in the decreasing order of ADM, THP and 4'-epi. Because of the low affinity to heart tissue and low aglycone production, 4'-epi and THP were considered to be improved in the cardiotoxicity.
  • 慈性 隆義
    1994 年 106 巻 11-12 号 p. 1177-1181
    発行日: 1994年
    公開日: 2009/03/30
    ジャーナル フリー
    The mitochondrial membrane fluidity in the rat liver and kidneys was measured by electron spin resonance spectrometry using spin labels 5- and 16-doxyl stearic acid (5-, 16-DS) after administration of an ethanol solution (7%) for 3 weeks. The order parameter, calculated from the 5-DS spectra, which is utilized for assessing the fluidity of the lipid bilayer near the surface of the membrane, increased significantly in the liver of the ethanol-administered group compared with the control group. No significant difference in the order parameter was observed in the kidney. On the other hand, in the motion parameter from the 16-DS spectra, which is utilized for the core of the lipid bilayer, no significant change was observed in either the liver or the kidneys.
    These findings indicated that the mitochondrial membrane fluidity decreased, by chronic ethanol adminitsration, in the lipid bilayer near the surface in the rat liver, but no fluidity change was observed in the rat kidney.
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