We compared the conventional hip prosthesis with a newly developed SH-type hip prosthesis by examining the degree of true roundness, sphericality, and surface roughness of the joint, as well as evaluating the metal stem properties. The roundness, sphericality and surface roughness were evaluated using Ogawa's criteria. Ogawa classified these values into 3 categories, A (5 points), B (3 points), and C (1 point), and the overall scores into 4 categories, A (21 points or more), B (20-18 points), C (17-15 points), and D (14 points or less). In class A, roundness was less than 0.1μm for the femoral head and less than 20μm for the acetabular cup, sphericality was less than 5μm and less than 50μm, and surface roughness less than 0.1μm and less than 0.5μm (maximal roughness of the cup was 5μm), respectively. Applying these criteria, the total score for the SH-type hip prosthesis placed it in class A. The strength and microcleanliness of the stem itself were sufficiently satisfactory.
Tumor infiltrating lymphocytes (TIL) were isolated from various human cancers and cultured. The expansion, cytotoxicity, phenotype, and perforin, which is considered the cytotoxic factor of killer cells, of TIL were examined. TIL cultured in 1000u/ml recombinant interleukin-2 (rIL-2) with 20% conditioned medium (CoM) exhibited far better expansion capability than that of TIL cultured in rIL-2 1000u/ml alone, and continued to expand up to 14 weeks. The maximal expansion index (EI) was 23912. When TIL arrested expansion during the culture, the addition of PHA restored the ability to expand. The CoM was composed of spent medium from a 3-day culture of peripheral blood mononuclear cells from cancer patients. The lymphokine-activated killer (LAK) cells induced by the culture was utilized for clinical adoptive immunotherapy in our department. Cytotoxicity against K562 and Daudi target cells was highest at 2 to 3 weeks, and disappeared between 4 and 7 weeks of culture. The phenotype of fresh TIL was mainly CD3+ T cells, predominantly CD4+ cells, but CD8+ cells became predominant in long term culture. The frequency of IL-2R+ and HLA-DR+ cells was correlated with the expansiveness of TIL. The appearance and intensity of perforin positive cells were associated with the level of cytotoxicity of cultured TIL.
The effects of enflurane and pretreatment with phenobarbital or pyridine on hepatic cytochrome P-450 (P-450) were studied in male rats. The rats without pretreatment and those pretreated with phenobarbital or pyridine, were fasted for 24 hours and exposed to 1.2% enflurane at 10% or 21% oxygen for 2 hours. Each group consisted of 6 rats, and rats that were not exposed to enflurane served as control. In the group of rats pretreated with pyridine and exposed to enflurane at 10% oxygen, the content of P-450 and activity of aniline hydroxylation decreased significantly to approximately 61% and 62% of those of the control, respectively. Increases in the plasma level of AST and ALT, and centrilobular necrosis in hepatic tissue were also seen in this group. Administration of disulfiram to rats pretreated with pyridine inhibited the decrease of P-450 and hepatic injury. These results indicate that P-450 induced by pyridine was decomposed by exposure to enflurane at 10% oxygen and might cause hepatic injury.