To cause tuberculous meningitis injected a little tubercle bacilli of human type into the spinal canal of rabbits. Some of them were infected by single injection, and the others after sensitized by 0.1-0.2 mg of live or died tubercle bacilli or 0.1cc of 1% tuberculin solution.
In the non-sensitized cases, all of 3 cases were infected with tuberculous meningitis by 1.0mg of tubercle bacilli, but 14 cases infected by less than 0.5 mg of bacilli were diverse acording to clinical and histological observation.
In the sensitized cases all of 18 cases in 3 groups were infected with tuberculous meningitis by 0.1-0.2mg of tubercle bacilli, especially were most effective for the sensitized rabbits infected by liver bacilli. 3 cases of them seemed recovered, but within 80-200 days recured and died.
Under histological observation, tuberculous meningitis of rabbits showed a strong tendency to productive inflammation by monocytes.
Miliary tuberculosis was caused by injecting tubercle bacilli into the left ventricle of rabbits' hearts after sensitized meningina with tuberculin solution. The symptom of menin-gitis did not appear, but under histological observation tuberculous degeneration could be found among some of them.

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By means of the reinfection method which has been reported in Part I, I caused tuberculous meningitis to rabbits, and used streptomycin in spinal canals and general treatmant.
Rabbits were divided into 3 groups for streptomycin treatment. viz, the 1st group for use 2 days before reinfection. the 2nd at the same day, the 3rd after the appearance of the symptom of tuberculous meningitis. and each group was further divided into 2 parts. the 1st for only local treatment, the 2nd for local and general treatment.
All of 15 cases of acute form died in 16 days. but they lived 10-15 days longer than the non-treatment cases. 7 cases of chronic form recovered and did not recur within 200 days.
Under histological observation, tuberculous meningitis of rabbits. which treated with streptomycin, showed disappearance of monocytes and increase of fibrous degeneration. And the pathological degeneration of meningitis was so light that streptomycin must have affected directly against tubercle bacilli.

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Roentgenograms of breasts in 35 cases of tuberculous meningitis patients were classified according to the principle of Prof. OKA.
The most important part of the primary forcus in tuberculous meningitis has been said to be tuberculosis pulmonum and hilus lymphatic gland. All the same with my cases.
Most of the complication cases to these patients were miliary tuberculosis (37.1%) followed by nodulous (acinous, productive) form, (17.1%) primary form, (14.2%)infiltrated form, (8.6%) etc. There were 4 cases in which any tuberculous observations were not found.

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Röntgenographic observation on normal intestinal canals was published by NONOMURA in the same clinic in 1941. In accordance with his method 13 cases of mesenterial disease were observed, comparing with 8 cases of other diseases; as controls, viz. diarrhoea, constipation, corpulence, ascites and abdominal tumour etc.
In mesenterial disease, there were 6 cases of swelling of mesenterial glands, 5 cases of peritonitis and 2 cases of cicatrical mesenteritis. By röntgenographic observation these cases were found to be in common with each other. Excretive time of contents in jejunum and ileum was delayed. Specific relief images-snowflake form, scattered cloudlet form-of jejunum appeared for most cases after 3-4 hours of taking contrast remedy. Ileum could not make intesitnal globe. And there were abnormal tonus of intestinal canal, as spastic parts and atonic parts were so confuse that the dimension of intestinal canal were seen very irregular. Differential diagnose from other intestinal disease was not so difficult.

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In order to investigate the effects of penicillin treatment in the course of syphilis, I have made observations on them from various standpoints in 75 cases of syphilis of every stadium, and obtained the following results:
1) By taking the average, spirochaeta pallida disappeared in 2.1 days, took 3.6 days for the granulation tissue to become fresh, 9.3 days to cure the chancre and 14 days for the induration to, disappear. Moreover, roseola disappeared in 9.3 days, papule in 7.2 days and in 6 to 10 days it showed an excellent effect on gumma.
2) The time needed for the disappearance of eruption depended on the amount of penicillin injected daily.
3) By examining the transition of the serum reaction in each stadium of syphilis, penicillin showed an exccllent effect on primary syphilis, and both in recent and latent secondary syphilis 10 cases of 14 turned negative or diminished in degrees, but in the late syphilis the effect of penicillin was not reliable.
4) Penicillin was also eflective on the so called anti therapeutic. syphilis in latent tertiary syphilis. syphilitie myelitis and congenital syphilis.
5) 5, 400, 000 to 6, 000, 000 units of penicillin as a total was the most effective quantity for penicillin treatment.
6) Injecting 600, 000 units of penicillin at once every day was the most effective application.
7) 13 cases out of 75 showed slight reation due to the penicillin injection.
8) These reations tended to appear at the initial stage of penicillin treatment.
9) 84.7% of the patients lost more or less weight during the treatment, especially the late syphilis cases, who lost more weight, and also, they were be found more frequeutly in the cases which showed improvement in the serum reation.
10) Albumin, glycose and urobilin did not appear in the urine during the treatment.
11) The increase of the daily urine quanity was found in the recent and latent secondary syphilis and the latent tertiary syphilis.
12) Penicillin improved the occult insufficiency of the liver function caused by syphilis.
13) No cases showed insufficiency of the liver function due to the penicillin treatment.
14) The general symtom caused by syphlilis took a favourable turn with penicillin trcatment.

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In order to examine the influence of penicillin on rabbits oculated with syphilis when penicillin was injected at the period when the complement combining reaction (WaR) turned positive and observed the course of the syphilitic local state and the disappearance of spirochaeta pallida from the local area, especially the transition of the serum reaction after the injection, and obtained the following results:
1) The term needed for the serum reaction to turn negative was reduced in all of the cases in which syphilis were oculated.
2) In the cases in which 50, 000 U. of penicillin were injected, WaR turned negative in 28.6 days, Murata's reaction in 39.3 days and Kahn's reaction in 45.6 days on average.
3) In the cases in which 100, 000 U. were injected WaR turned negativ in 38.5 days, Murata's reaction in 52.5 days and Kahn's reaction in 66.5 days on average.
4) In the cases in which 200, 000 U. were injected, the influence of the injection to the serum reaction appeared rapidly and the serum reaction turned negative in a comparatively short time, i.e., WaR in 21.3 days, Murata's reaction in 34.6 days and Kahn's reaction in 39.3 days.
5) Concerning the local state (syphilitic orchitis), the inflammation was absorbed in 22.8 days in 50, 000 U. injected cases, in 19 days in 100, 000 U. injected cases and in 13.3 days in 200, 000 U. injected cases.
6) As to the disappearance of spirochaeta pallida from the local area, it could not be detected in 2.3 days in the 200, 000 U. injected cases, in 8.6 days in the 50, 000 U. injected cases and in 7.7 days in the 100, 000 U. injected cases.
7) In the demonstration of spirochaeta pallida, it disappeared comparatively rapidly in the left rather than the right testicle.
8) The recurrens of the serum reaction and the local state could not be found.
9) In short, a definite and regular influence of penicillin could not be found in the 50, 000 U. or 100, 000 U. injected cases, and moreover, no difference between the 2 groups was demonstrated.
10) In the cases in which 200, 000 U. were injected, a definite and remarkable influence of penicillin could be recognized.

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I have injected penicillin in rabbits in which spirochaeta pallida was oculated at the period when the precipitation (Murata and Kahn) turned positive and observed the transition of the serum reaction, alteration of the local state and disappearance of spirochaeta from the local area and obtained the following results:
1) In the cases in which 50, 000 U. of penicillin were injected, the influence of penicillin on the 3 reactions (WaR, Murata and Kahn) was rather unstable and the term needed for the 3 reactions to turn negative was prolonged, i.e., WaR turned negative in 66.5 days after the oculation (54.5 days after the injection), Murata's reaction in 94.5 days (82.5 days after the injection), and Kahn's reaction in 91.0 days (79.0 days after the injection).
2) In the 100, 000 U. injected cases, WaR turned negative in 63.0 days after the oculation (50.7 days after the injection), Murata's reaction in 84.0 days (71.7 days after injection), Kahn's reaction in 92.8 days (80.5 days after injection).
3) In the cases in which 200, 000 U. were injected, a definite effect of penicillin on the serum reaction was observed when compared with the previous 2 groups, i.e., WaR turned negative in 11.6 days after oculation (7.0 days after injection), Murata's reaction in 28.0 days (18.6 days after injection), and Kahn's reaction in 46.6 days (37.3 days after injection).
4) In the observation of the effect of penicillin on the local state: in 50, 000 U. injected cased, stuntedness of the local state and the prolongation of the incubation could be observed and the local state cured in 36.0 days, in 100, 000 U. injected cases, the prolongation of incubation could be observed but the local state occured and took 25.7 days to disappear and in 200, 000 U. injected cases, the local state was completely prevented and the syphilitic local symptoms did not appear.
5) 200, 000 U. of penicillin showed a definite effect on the existence of spirochaeta, i.e., the spirochaeta conld not be detected at all, and in 50, 000 U. injected cases, the effect of injection was not distinct and they disappeared in 16.0 days in the former cases and in 9.2 days in the latter cases.
6) The recurrens of the serum reaction and the local state could not be observed during the observation of 4 months on the average. Comparing the above results with those described in Part II, where the injections were given at the period when WaR turned positive, the following results were obtained:
7) Concerning the 50, 000 U. and 100, 000 U. injected cases, the term needed for the serum reaction to turn negative was prolonged in the cases in which the injections were given at the period when precipitation turned positive, when compared with the cases in which injections were given at the period when WaR turned positive, therforee the time required for the absorption of local state and the disappearance of spirochaeta was also prolonged.
8) In the observation of 200, 000 U. injected cases: WaR turned negative in 42.6 days after oculation (21.3 days after inlection), Murata in 56 days (34.6 days after injection), Kahn in 60.6 days (39.3 days after injection) in the cases in which injections were given at the period when WaR turned positive, and in the cases where injections were made at the period when precipitation turned positive, WaR turned negative in 11.6 days after oculation (7 days after injection), Murata in 28.0 days (18.6 days after injection), Kahn in 46.6 days (37.3 days after injection), i.e., distinct reduction could be observed in the latter cases.
9) In 200, 000 U. injected cases, a remarkable and regular effect of penicillin could be observed when compared with other 2 groups, and especialy in the cases in which injections were given at the period when the precipitation turned positive the effect was more distinct.
10) In 50, 000 U. and 100, 000 U. injected cases no distinct and regular effect of penicillin could be observed.

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Nous avons de nouveau examiné histologiquement ce que nous avons déjà observé macroscopique au chapitre précédent.
Au sujet de la transplantation des poils autogénes, nous avons poursuivi les qualités de changements aux noyaux des cellules.
Quant à la coloration à la transplantation des poils hétérogénes, nous avons exécuté la méthode de Feulgen, coloration d'hématoxyline-éosine.
De ces expérimentations en question, nous avons obtenu les memes résultats qu'au chapitre précédent, et après tout ces résultats ont histologiquement démontré les expérimentations au chapitre précédent.
Le Cétoénollipoïde était le plus souvent apercu à la borne entre la partie nécrosée de morceaux transplantés et celle qui en faisait exister encore, et de plus il était aussi apercu aux jeunes cellules et aux cellules dégénérées, mais dans trente jours environs il a diminué, en devenant cétoénolgranule, si évidemment qu'il a disparu en y adittionant de la barytelessive, et encore nous avons y apercu la résurrection de la fonction des cellules.
Au point de vue des changements déjà expliqués, nous avons observé que la dégénération légère aux cellules et la résurrection de la fonction de bonne heure étaient à la transplantation des poils disposés dans les deux verres du sérum artificiel séparément ajouté de la vitamine C et de la vitamine B₆.
Si nous arrangeons les résultats par degrés, d'abord c'est au résultat obtenu au sérum artificiel ajouté de la vitamine B₂, ensuite ce sont également les résultats obtenus dans les trois verres du sérum artificiel, du sérum séparément ajoute de la vitamine A et de la vitamine B₁, et les exemples qui étaient flottés dans du sérum artificiel séparément ajouté de la vitamine D étaient le moins valuables.
Et en outre, quand nous avons comparé les deux résultats des peaux poilues flottantes dans chaque solution pendant une heure et trois heures, le dernier était considérablement inférieur au premier. Et de plus, l'état du recouvrement de la fonction des cellules au dernier était en retard de quatorze à vingt jours environs.
A l'examen de la transplantation de la peau poilue hétérogéne, les cellules dela gaine épithériale der poil sétaient montré négatives à la réaction de Feulgen dans dix jours et aprés cela elles l'étaient successivement, et ainsi nous n'avons pu apercevoir d'un poil.
L'épithélium de la peau transplantée était négative de la première période à la réaction de Feulgen. L'épithélium qui a développé de la partie transplantée de la peau était montré positif dans quatre jours, et il a fixé complétement la cicatrice dans la partie transplantée de la peau poilue dans dix jours.

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Among the two same kind immune serum, I deemed one of them antigen and heaped up it upon another, and consequently precipitation of it was positive.
In a word, it seems to include antigen in antibody.
Therefore, when antibody immuned with Coli bacillus is heated, it seems to produce the paradoxe phenomenon compared with Coli bacillus antigen in correspondence for heat.

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Having taken observation on the resting current as hydrostatic high pressure has been evenly imposed upon isolated muscle (frog's gastrocnemius), obtained the following results;-
Due to the high pressure, injury potential of skeletal muscle decreases remarkably, while electric conductivity of any muscle indieafes a great inerease.
These facts may probably ascribed to the augmented permeabilily of plasma membrane, due to high pressure.

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In case certain high pressure (400 atm.) is imposed on excised skin of a frog, its resting potential abates itself considerably, while its electric conductivity increases great deal.
These facts may probably be ascribed to the augmented permeability of plasma membrane of the epithelium, due to high pressure.

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In case any high pressure (that amounts to 400 atm.) has been directed toward isolated frog's skin, electric pressure of salt current reduces considerably.
These facts may probably ascribed to the augmented permeability of plasma membrane of the epithelium, due to high pressure.

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When some high pressure (1500 atm. at maximum) is applied to various living celltissue, (such as, red corpuscle, bladder, mesenterium, plant cell etc.), their electric conductivity may greatly be enhanced.
These facts may probably ascribed to the augmented permeability of plasma membrane, due to high pressure.

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If high pressure(almost 2000 atm. or so)has been applied to any plant cells(Tradescantia), its plasmolysis suffers some difficulty.
This may be ascribed to the abatement of difference ofas motic pressure due to augmented permeability of plasma membrane by high pressure.

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In case we observe vital staining of plant cells (Trades cantia, Allium cepa, Spirogyra) with brilliant cresyl blue as pH-indicator, pH of its basic stain solution proves to augment owing to high pressure (up to 2000 atm.).

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In case any living staining of plant cell (Trades cantia, Allium cepa, Spirogyra) should be observed as pH-indicator, the pH of neutral red solution increases, owing to high pressure.

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The blood level of Vitamin B₁ in 80 cases of various dermatologic disorders was defermined with thiochrome method using permutit and considering the value of the healthy individuals and the results of other investigators, the minimum limit was established on 6.0γ% and the following results were obtained:
1) In 17 cases of healthy individuals, the average of the blood level of total Vit. B₁ was 8.34γ%, and it presented lower value in summer rather compared with in autumm. The average of the blood level of Vit. B₁ in dermatologic disorders was 6.51γ%, i.e., 1.83γ% lower than in the healthy individuals.
2) Concerning eczema acutum, it showed normal value in erythematous and desquamatous stadium. but in the rest of the stadium most of them showed lower value, in eczema chronicum it also showed lower value in cases in which the course was prolonged.
3) In dermatitis acuta and Duhring's dermatitis, the blood level of Vit. B₁ decreased in cases in which excessive exsudation could be seen, pemphigus, Domian enanthem and pellagra showed obvious deficiency of Vit. B₁. Moreover, in most of the cases of erythematodes and psoriasis vulgaris showed Vit. B₁ deficiency, but in acne vulgaris, alopecia arezta, chloasma and erythema induratum Bazin showed normal value.
4) Vit. B₁ takes part in the metabolism with close relation with the functions of many organs, as well as with other Vitamins and endocrine system; so the variation of the blood level of Vit. B₁ might not be attributed as the only cause of the previous diseases, but it could be concluded that in some of the cases, the deficiency of Vit. B₁ takes an important roll in the cause or degeneration of dermatologic diseases.

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According to the fact that the Synthesis of Co-carboxylase from Vit. B₁ takes place in the liver, and in the case of Vit. B₁ deficiency the insufficiency of the liver function could often be observed, therefore. the close relation between Vit. B₁ and liver function could be presumed. I have examined the relation between Vit. B₁ and liver function in 71 cases of various dermatologic diseases, according to Takata's reaction, hepatosulphalein method and urobilinogen reaction in urine, as the examination of liver function and obtained the following results.
1) 55.6% of the cases in which the blood level of Vit. B₁ was below 6.0γ% showed liver dysfunction, especially the allergic diseases such as eczema, urticaria and prurigo tended to liver dysfunction, and the similar tendency could also be seen in Duhring's dermatitis, pemphigus and pellagra.
2) 66.7% of the cases in which the blood level of Vit. B₁ was above 6.0γ% showed normal liver function, and chloasma, alopecia areata, verrucae planae juveniles and trichophytia showed normal function.
3) In acne vulgaris, erythema induratum Bazin, erythematodes and psoriasis vulgaris no definite relation between Vit. B₁ and liver function could be observed.
4) Including allergic dermatoses, in the dermatologic diseases which tend to exsudation, both positive urobilinogen reaction and deficiency of Vit. B₁ could be observed in many cases.
5) Among the 3 reactions for the examination of liver function, the hepatosulphalein method concured closely with blood level of Vit. B₁.
6) Approximately 90% of the cases had both normal liver function and normal Vit. B₁ value, and 90% with liver dysfunction and deficiency of Vit. B₁.
Etiology of many skin diseases are still unknown, and in the relation of Vit. B₁ deficiency and the liver dysfunction to the cause of various dermatologic disorders, the cause and the result are also not clear, but according to my experiments it could be said that the Vit. B₁ deficiency and the liver dysfunction play an impostant roll in the cause and course of some dermatologic disorders.

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Sawada's urinary pyruvic acid reaction, which is known as indirect diagnostic method of Vit. B₁ deficiency, is widely applicated in various medical disorders, but in my researches in dermatologic area I could not find of this reaction. By applicating Sawada's reaction in dermatodes, following results were obtained:
1) In 66 cases of 115 patients, this reaction appeared positive (57.4%) i. e., about 20% higher than normal persons (36.0%). Showing positivity in main diseases.
In eczema chronicum 75%, dermatitis acuta 71.4%, ephelides 60.0%, chloasma 57.4%, urticaria and erythema induratum Bazin 50.0% each. alopecia areata 42.8%, acne vulgaris 37.5%, appeared positive. while in psoriasis vulgaris and trichophytia only 25.0% appeared positive.
2) As liver function tests, Takata's reaction, Hepatosulphalein method and urobilinogen reaction in urine were taken and in most of the cases in which Sawada's reaction appeared negative, these 3 reactions were also negative, but when Sawada's reaction appeared positive, these 3 reactions appeared positive only in a few case. therefore the deficiency of liver function can not be presumed from the positive appearance of this reaction. Determining the liver function from the previous 3 reactions, in the relation between the liver function and Sawada's reaction, 80.0% of the cases with liver dysfunction showed positive reaction of this test.
3) Determining the relation between the blood level of Vit. B₁ and Sawada's reaction, the average of the blood level of Vit. B₁ in the negative cases of this reaction was 7.49γ%. and that of the positive cases was 5.57γ%, and definite difference between them could be observed.
Classifying the results of the blood level of Vit. B₁ in two groups, i.e., below 6.0γ% as deficiency and above 6.0γ% as normal value, in the normal cases 58.33% appeared negative in Sawada's reaction, therefore it could not be conclunded that in the cases which the blood level of Vit. B₁ appears normal, Sawada's reaction appears negative.
4) 82.35% of the cases in which Sawada's reaction appeared negative, showed normal blood level of Vit. B₁, while only in the half of the positive cases I found this level below 6.0γ%. So when we apply this reaction in clinical examination, it is misleading to determine Vit. B₁ deficiency by the positive appearance of this reaction.

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