The author has investigated the method of measuring the respiration and glycolysis of sternal punctate by using Warburg's manometer. The results obtained can be summarized as follows:
1. When punctured with strong suction force and the punctate was taken in a little quantity (0.3cc), the stable and large respiration value was observed.
2. The respiration of cells in the sternal punctate was more injured at the second punctate in two successive suction than glycolysis action.
3. 1.0 cc of suspension medium was higher in respiration value than the case of 2.0 cc.
4. When the so-called “dioxalate” (Potassium oxalate 0.75; Ammonium oxalate 1.25; distiled water 100.0) was used as anticoagulant the highest respiration value was obtained. Heparin being next, sodium citrate showed considerable low value.
5. In the case of the normal person, the fat-free dry weight was constant, having little relation to the increase or decrease of the nucleated cell count. But in the various diseases, there turned out not always to be a positive correlation between the former and the latter.
When the action of respiration and glycolysis of sternal punctate was measured, in synthesizing the above suitable conditions, the value was stable and large. Therefore the author has confirmed that the measuring method of respiration and glycolysis of sternal punctate in this experiment has the possibility of clinical application.
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