巻号一覧
71 巻 (1959)
- 12-2 号 p. 8243-
- 12-1 号 p. 7861-
- 11supplement 号 p. 1-
- 11-2 号 p. 7531-
- 11-1 号 p. 7095-
- 10-2 号 p. 6691-
- 10-1 号 p. 6223-
- 9-2 号 p. 5773-
- 9-1 号 p. 5405-
- 8-2 号 p. 4993-
- 8-1 号 p. 4515-
- 7-2 号 p. 4069-
- 7-1 号 p. 3623-
- 6-2 号 p. 3149-
- 6-1 号 p. 2783-
- 5-2 号 p. 2423-
- 5-1 号 p. 1989-
- 4-2 号 p. 1655-
- 4-1 号 p. 1145-
- 3supplement-2 号 p. 1-
- 3supplement-1 号 p. 1-
- 3-2 号 p. 1145-
- 3-1 号 p. 691-
- 2-2 号 p. 691-
- 2-1 号 p. 323-
- 1 号 p. 1-
前身誌
70 巻, 1 号
選択された号の論文の27件中1～27を表示しています
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第4篇 電気衝撃並にインシュリン衝撃施行大黒鼠脳髓homogenateのグルタミン酸・グルタミン・γアミノ酪酸の相互関係について伊原 可能1958 年 70 巻 1 号 p. 1-8
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーThe author measured quantitatively glutamic acid, glutamine and γ-aminobutyric acid in the rat brain homogenate by paperchromatography, and obtained the following results.
1) When the brain homogenate of normal rat is incubated at 38C° for 60 minutes under anaerobic conditions, the quantity of glutamic acid decreases slightly while the quantities of glutamic acid and γ-aminobutyric acid increase. When glutamic acid is loaded to the brain homogenate as substrate, the glutamic acid so loaded decreases markedly whereas γ-aminobutyric acid increases markedly.
2) In the case of the homogenate subjected to repeated electro-shocks, such increase in γ-aminobutyric acid is lessened.
3) In the case which received repeated insulin shocks, the increase in γ-aminobutyric acid likewise tends to be reduced.
4) These facts seem to indicate that the glutamic decarboxylase acitivity in the brain in the cases (2 and 3) mentioned above might have been slackened.抄録全体を表示PDF形式でダウンロード (437K) -
第1編 再生不良性貧血患者骨髓の呼吸解糖作用並びに同患者赤血球の呼吸作用について西内 道雄1958 年 70 巻 1 号 p. 9-21
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリー1) Following the classification of Prof. Hiraki the author divided hypoplastic anemic patients into five types and carried out the following studies. Oxygen consumption and glycolysis of the sternal bone marrow aspirated from hypoplastic anemic patients generally showed a decrease but the degree of the decrease varied according to the type of anemias, namely, the type of blood cell arrest showed the value close to that of the normal person, followed by type of maturation arrest and type of regeneration disturbance, and mixed form as well as type of pan-myelophtisis showed markedly low values.
2) The oxygen consumption (i.e. O2-consumption values) of peripheral erythrocytes of hypoplastic anemic patients was recognized to be lower than that in the normal person. Moreover, it was found that the in vitro addition of various substances that reactivate the respiratory action of erythrocytes showed lower values in oxygen consumption of erythrocytes of hypoplastic anemic patients.
3) It has been proved that the serum of hypoplastic anemic patient in vitro has an inhibitory action against the O2-consumption of erythrocytes of normal persons.抄録全体を表示PDF形式でダウンロード (1097K) -
第2編 再生不良性貧血患者血清が家兎骨髄呼吸解糖作用に及ぼす影響について西内 道雄1958 年 70 巻 1 号 p. 23-33
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリー1) By successive injections of serum of hypoplastic anemic patient into normal rabbits for a long period of time marked anemia was induced in the animals, but by similar treatment with the serum of normal person no such phenomenon could be observed.
2) As for the values of O2-consumption and glycolysis of the bone marrow of the rabbits injected with serum of normal person, no change occurred in the myelogram of the group receiving injections for 1-2 weeks at the same time these myelograms compared with those of normal rabbits without such treatment showed no significant difference, and in the group receiving the injections for three weeks excitatory picture as well as acceleration of oxygen consumption were observed.
3) On the contrary, in the group of rabbits receiving the serum of hypoplastic anemic patients for 1-2 weeks the percentage of basophilic erythrocytes in their myelograms increased as well as an acceleration oxygen consumption, while in the myelogram of the group receiving injections for three weeks a picture of maturation arrest and decline in the values of oxygen consumption and glycolysis, oxygen consumption in particular, were obsorved.
4) It has been proved that the serum of hypoplastic anemic patient in vitro has an inhibitory action against the O2-consumption of the bone marrow of normal rabbit.抄録全体を表示PDF形式でダウンロード (884K) -
第3編 コラルゴール貧血家兎骨髄及びCo60照射家兎骨髄の呼吸解糖作用について西内 道雄1958 年 70 巻 1 号 p. 35-48
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリー1) Progressive anemia was induced in the rabbits injected with collargol, and reticulocytes and nucleated erythrocytes in blood first increased and then gradually decreased. Myeolgram and tissue picture of their bone marrow presented first excitatory proliferation picture followed by regeneration arrest.
2) As for the values of oxygen consumption and glycolysis of bone marrow of the rabbits made anemic by collargol, in the group given 4 day injections they were enhanced while in the group receiving 4 week injections they were reduced markedly. Values of oxygen consumption and anaerobic glycolysis of each group when compared with respective values of normal rabbits showed statistically significant difference.
3) When collargol solution was added to the bone marrow of normal rabbit directly in vitro, the O2-consumption tended to increase slightly in the case when the concentration of the collargol solution was low but it decreased gradually as the concentration grew higher.
4) When the serum of rabbits made anemic by collargol was added directly to the bone marrow of normal rabbit in vitro, oxygen consumption of the bone marrow showed a tendency to increase.
5) In the rabbits irradiated with Co60, reticulocytes and leucocytes decreased strikingly and in their myelogram a picture of regeneration arrest in the myelocytic cells could be observed.
6) Although no significant difference in O2-consumption between the bone marrow of the rabbits irradiated with Co60 and that of normal rabbits could be observed statistically, the values of glycolysis was reduced markedly and when compared with those of the normal rabbits a significant difference could be seen.
7) When the serum of rabbits irradiated with Co60 was added directly to the bone marrow of normal rabbit in vitro, the O2-consumption of the bone marrow of normal rabbit was increased.
8) From these findings it has been clarified that the sera of the rabbits either made anemic by collargol or irradiated with Co60 have not an inhibitory action against O2-consumption of the bone marrow of normal rabbit such as the sera of hypoplastic anemic patients.抄録全体を表示PDF形式でダウンロード (2371K) -
第1編 急牲瀉血が家兎大腿骨々髄の増生面積に及ぼす影響に就て沼本 徹郎1958 年 70 巻 1 号 p. 49-61
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーThe author investigated from various angles behaviours of bone marrow of rabbits in acute bleeding anemia by bone marrow tissue culture.
After acute bleeding of 20 cc./kg. of each rabbit, the recovery mechanism in the bone marrow has been compared with that of the normal rabbits by measuring the relative growth rate and the index of cellular density of the bone marrow in hanging drop culture at intervals of 1, 3, 5, 8, 12 and 24 hours as well as 2, 3, 5, 7, 10, 14 and 28 days after bleeding. It has been found that the growth area begins to decrease inmmediately after bleeding and shows the minimum value 5 hours after bleeding, but 12 hours afterwards it starts increasing and relative growth rate 24 hours after bleeding has increased 1.80 times that of the control; 2 days later 2.03 times; 3 days afterwards 2.29 times; 5 days later 2.37 times; and the maximum of accerelation of bone marrow mechanism has been found around 3-5 days after bleeding.
The growth rate, which thus reaches the maximum 5 days after bleeding, begins to decrease thereafter and becomes nearly equal with that of the normal rabbits around 10-14 days afterwards.抄録全体を表示PDF形式でダウンロード (922K) -
第2編 急性瀉血が家兎大腿骨々髄の偽好酸球機能に及ぼす影響に就て沼本 徹郎1958 年 70 巻 1 号 p. 63-81
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーThe author studied the function of pseudoeosinophils of the femur bone marrow in acute bleeding rabbits from the standpoints of wandering velocity, phagocytosis of carbon particles and vital staining of neutral red; and obtained the following results.
1) Owing to the release of depot blood cells in sinus of bone marrow, the wandering velocity of pseudoeosinophils in the bone marrow slightly increases immediately after and 1 hour after bleeding, but 3-5 hours after bleeding some decrase in wandering velocity and phagocitosis of carbon particles, and deeper staining of vital staining of neutral red can be recognized, namely, some decline in the function of bone-marrow pseudoeosinophils.
2) The wandering velocity of pseudoeosinophils as compared with that in the control starts increasing around 12 hours after bleeding, the time when white corpuscles are repro duced, and the increase in the wandering velocity reaches its maximum at the time when the function of bone marrow is at its best, namely, around 3-5 days after bleeding, while the phagocitosis of carbon particles reaches its lowest level and vital staining of neutral red becomes rapid and deep; and moreover, its decoloration ensues quite rapidly as well. Namel y, morphologically mature as they are, nevertheless, these newly produced white corpuscles can be considered as an execessive production of blood cells possessing a peculiar dissociation of function, which is functionally immature.抄録全体を表示PDF形式でダウンロード (12338K) -
第3編 急性瀉血が家兎諸骨々髄の造赤血球系機能に及ぼす影響に就て沼本 徹郎1958 年 70 巻 1 号 p. 83-96
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーThe author studied the behaviors of bone marrow in acute bleeding rabbits by fluid culture of bone marrow and obtained the following.
1) Increasing rate of red cells immediately after bleeding is the same as that of the normal, but it begins to decrease 1 hour afterwards; and it shows the minimum value 8 hours later. However, it begins to rise 12 hours after bleeding; and its rate is 1.59 times that of the control 2 days after bleeding, 2.38 times after 3 days; 2.55 times after 5 days; 1.98 times after 7 days; 1.50 times after 10 days. Namely, the muximum rate of increase in the erythrocytes is reached on around 3-5 days after bleeding and 14 days after it recovers more or less to the normal level.
2) Increasing rate of hemoglobin as compared with that in the control shows a decrease 1 hour after bleeding and it is lowest around 8-12 hours afterwards; but it begins to increase around 24 hours and shows the maximum value around 5-7 days after bleeding, while on the 14th day it practically coincides with that in the control group. However, the rate of production of hemoglobin seems to be less than that of red cells.抄録全体を表示PDF形式でダウンロード (4175K) -
岡村 安政1958 年 70 巻 1 号 p. 97-103
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーIt has been discovered that there exists, within the fetus serum, as well as human placenta, a similar specific protein that one cannot find out in any human serum; which has been considered to have been formulated in placenta; further, one can probe out the abovesaid unique protein part in a show alike.抄録全体を表示PDF形式でダウンロード (567K) -
第1報 夏季土壤中に於ける鉤仔虫の垂直分布に関する実験的研究小武守 靖1958 年 70 巻 1 号 p. 105-110
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーIn the experiments carried out for the purpose of finding out the manner of hatching of hookworm eggs embedded in various layers of the soil in farm fields throughout the Okayama Prefecture during summer months and to see in what different depths these larvae are found under various conditions, the author obtained the following results.
1) Hookworm larvae hatch readily and their longevity of life seems to be well over eight weeks.
2) In fair weather these larvae generally avoid the layer close to the surface of ground between the hours, noon to 2 o' clock in the afternoon and seeking refuge in a cooler layer of the soil, more larvae are found in the deeper layers. However, on rainy days these larvae migrate to the layer close to the surface in large numbers.抄録全体を表示PDF形式でダウンロード (2305K) -
第2報 晩秋土壤中に於ける鉤仔虫の垂直分布に関する実験的研究小武守 靖1958 年 70 巻 1 号 p. 111-115
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーIn the experiments conducted for the same purpose as in report 1, regarding the manner of hatching of the hookworm eggs embedded in various layers of soil in farm fields in the Okayama Prefecture during the latter part of fall and to see in what different depths these larvae are found under various conditions, the following results were obtained.
1) Hookworm larvae are found in relatively deeper layers in the early morning hours, but in the daytime some of them migrate to the upper layer where the temperature is comparatively high.
2) Hookworm larvae and their eggs all die in the beginning of winter and none survives over winter.抄録全体を表示PDF形式でダウンロード (329K) -
第3報 若菜病発生畑地土壤中に於ける鉤仔虫の垂直分布に関する研究小武守 靖1958 年 70 巻 1 号 p. 117-122
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーIn the investigations carried out for the purpose of determining the vertical migration of hookworm larvae found in the soil of farms where “Wakana disease”, the so-called young green disease occur and numbers of hookworm larvae which adhere to young green leaves in these farms, the following results have been obtained.
1) In the morning and the daytime, more hookworm larvae are found in deeper layers of the soil of the farm where the young green disease is known to occur.
2) In the case there are numerous hookworm larvae in the field where the young green disease is known to occur, naturally more hookworm larvae are found adhering to young leaves growing in the field.抄録全体を表示PDF形式でダウンロード (372K) -
小田 琢三, 関 周司, 柴田 高志, 酒井 晃, 岡崎 博明1958 年 70 巻 1 号 p. 123-135
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーDespite the excellence in bringing about reactions, there are still many difficulties and discrepancies in the use of neotetrazolium chloride for measuring the activity of succinic dehydrogenase system of tissue homogenate. Therefore, with the purpose to find out a measuring method simpler and more accurate, the authors performed some experimets, first on the enzymic reactions of tissue homogenate and then after stopping these reactions by using various agents, tested the ease with which reduction products are extracted with various solvents, and finally compared the absorption spectra of these liquid extracts with those of the products reduced chemically by sodium hydrosulfite. As the results, the extraction of the reduction products with acetone-ether (mixed in equal proprtion v/v) after stopping the reaction with 10 per cent formalin solution was found to be the simplest and best method, and moreover, the absorption spectra of extracts of the products obtained by enzymatical or chemical reduction proved to be uniform and the both of which showed the maximum absorption at the wave length of 520 mμ.
In addition, the concentrations of sodium succinate and neotetrazolium chloride, the content and kinds of tissues, pH values and concentration of phosphate buffer solutions, the duration of reaction, aerobic or anaerobic conditions, various factors either enhancing or in hibitory to reaction, were all regulated absolutely or relatively; and by drawing absorption curves of the reduction products, these enzymatic reactions were analyzed and scrutinyzed Then the authors established a method which is general, simple and accurate in measuring the enzymatic activities of a small quantity of fresh tissues in contrast with those of hist chemically stained specimens.抄録全体を表示PDF形式でダウンロード (3201K) -
50%凝集価の概念にもとずくWeil-Felix反応の吟味梶木 郁夫1958 年 70 巻 1 号 p. 137-152
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーFor the diagnosis of rickettsial diseases, though there are agglutination and complement fixation tests with the use of pure rickettsial suspension, the Weil-Felix test is still of high value for its technical simplicity and specificity. Even in the case of Tsutsugamushi disease, Fletcher, Wolf, Felix and Kawamura applied the Weil-Felix reaction with the use of Proteus OXK bacilli, and since then this reaction has been discussed in various ways, but the true antibody titer seems not to be obtained because of the insufficient consideration of the antigen concentration. Especially when the variation in the production of antibody is discussed on the basis of antibody titer, there apt to be misinterpretations.
By the Weil-Felix reaction with the use of Proteus OXK dacilli suspension of 50% agglutination titer as the antigen, the author examined the variation of the antibody production in the sera of the test animals inoculated with rickettsiae, in parallel with the case in which the antigen was selected at random in the conventional way.
As the result, it was found that when the antigen of 50% agglutination titer was used, no variation was shown in the antibody titer, irrespective of the sites of inoculation, the number of times of inoculation, life or death of rickettsiae, while, in the case of antigen selected at random, variable titers were yielded.
From these data, the author believes that the antigen concentration suitable for she Weil-Felix reaction has been found.抄録全体を表示PDF形式でダウンロード (1246K) -
第1編 粗ワクチンによる感染防禦に関する研究高橋 秀1958 年 70 巻 1 号 p. 153-161
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーImmunization with inactivated vaccines of Rickettsia tsutsugamushi has not been succeeded as yet, and also no definite result has not been reported on its inactivated vaccines.
The author studied the prophylactic effect of crude vaccine of R. tsutsugamushi. The test agents were Tanizawa and Mitani strains of R. tsutsugamushi separated and identified in the eastern part of Kagawa Prefecture. The formol-vaccine of the two strains was made of the peritoneal exudates containing a great deal of the agents which were obtained by intraperitoneal inoculation of rickettsiae into guinea-pigs previously injected with chlorpromazine. Mice were used as the test animals. The results were as follows:
1) The vaccine tested did not show any toxic effect against mice, and the vaccinated mice did not show any symptomes or signs of the disease or accidental death.
2) The mice thus vaccinated showed apparently the resistance to the attack of the homologous strain.
3) Against the heterologous strain, the resistance to infection was observed to some extent, but was far inferior compared with that against the homologous one.
In short. the infectious protection by the formol-vaccine was highly strain-specific, and showed a high resistance against the attack of the homologous strain.抄録全体を表示PDF形式でダウンロード (639K) -
第2編 各種の薬剤による不活化の研究高橋 秀1958 年 70 巻 1 号 p. 163-172
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーAs for the resistance to the infection of Rickettsia tsutsugamushi, the immunization with inactivated vaccine had been previously reported. In the present report, the methods to inactivate the vaccine, which are closely relating to its effect, were studied. Tanizawa strain of R. tsutsugamushi separated in the eastern part of Kagawa Prefecture was used as the test agent, and was inactivated with formalin, phenol and merzonine. The results were as follows:
1) The formol-and phenol-vaccine showed little toxic effect, and any symptomes or signs of the disease did not appear in the mice immunized with these vaccines. No accidental death due to the toxic effect of the drugs used for inactivation was observed.
2) The immunization with merzonine-vaccine showed a significant effect, but the toxic effect of merzonine also considerably appeared.
3) Both of formol- and phenol-vaccine showed a significant infectious protecting effect and did not cause any accidental death, but formolvaccine seemed to be more suitable for imminization.
In short, of all the inactivated vaccines of R. tsutsugamushi, the formol-vaccine was the most excellent and the phenol-vaccine the next.抄録全体を表示PDF形式でダウンロード (629K) -
第1編 恙虫病病毒感染ハツカネズミにおける所謂「リケッチア」細胞の本態に関する研究牛田 達之1958 年 70 巻 1 号 p. 173-185
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーIn mice infected with Rickettsia tsutsugamushi and suffering from the disease, there appears the peritoneal exudate containing the so-called “rickettsial cells” in which many rickettsiae are observed. The essence of the so-called “rickettsial cells” remains, however, as yet unexplained.
In the present series of experiments, the author tried the cytological classification of the “rickettsial cells” in detail as well as the interrelation between these cells and rickettsiae.
As a result of these experiments, it became clear that the so-called “rickettsial cells” consisted of both of the serosal cells in which rickettsiae were multiplying actively and the histiocytes which gormandized the multiplied rickettsiae; this gives a new definition of the so-called “rickettsial cells.”抄録全体を表示PDF形式でダウンロード (3954K) -
第2編 恙虫病病毒が示す染料に対する態度に関する研究牛田 達之1958 年 70 巻 1 号 p. 187-195
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーFor the staining of Rickettsia tsutsugamushi, several methods have been reported, of which Giemsa's method has been most widely employed. Even this Giemsa's method has been, however, proved not so satisfactory; this method takes a long time, and is not so useful to find the extracellular rickettsiae because of confusing precipitates, and even the rickettsiae unstaible with Giemsa's stain have been reported. Because of these facts, a new useful method is in need nowadays.
By employing Giemsa's method, the author already found the significant differences between the shapes of the rickettsiae in serosal cells and those in histiocytes. In this report, the author reinvestigated the hitherto reported several staining methods, and, at the same time, invented some new methods, fuchsin-azure, modified victoria blue and amido-black stainings, by which the morphological strucure of rickettsiae as well as the essential nature of Giemsaunstainable ones was clarified.抄録全体を表示PDF形式でダウンロード (3100K) -
第1編 恙虫病病毒の精製に関する実験大西 譲1958 年 70 巻 1 号 p. 197-209
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーIn the present experiments, Mitani and Tanizawa strains, which had been isolated in Kagawa Prefecture, were used. Rickettsiae of each strain were purified with kaolin and bentonite. The results were as follows:
1) In the purification of rickettsiae with kaolin, the adsorption on kaolin at pH 5.0 and the dissociation at pH 7.2 gave the purified rickettsiae in the largestest quantity, and thus purified rickettsiae showed a high antigenicity in the complement fixation test.
2) In the purification with bentonite, the adsorption at pH 4.5 and the dissociation at pH 7.2 gave the purified rickettsiae in the richest amount, and the purified rickettsiae thus obtained showed a high antigenicity in the complement fixation test.抄録全体を表示PDF形式でダウンロード (809K) -
第2編 精製恙虫病病毒の抗原牲の実験大西 譲1958 年 70 巻 1 号 p. 211-220
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーIn the present experiments, Mitani, Tanizawa, Ohzeki and Shichito strains of Rickettsia tsutsugamushi were used. Rickettsiae of each strain were purified with kaolin, and their antigenic properties in the complement fixation test were investigated in detail. The results were as follows:
1) In the complement fixation test between the purified rickettsiae of Mitani strain and the antiserum of Mitani or Ohzeki strain, marked complement fixation was observed.
2) In the complement fixation test between the purified rickettsiae of Tanizawa strain and the antiserum of Tanizawa or Shichito strain, complement fixation was observed to a high degree.
3) The purified rickettsiae of Ohzeki strain showed marked complement fixation in the antiserum of Ohzeki or Mitani strain.
4) The purified rickettsiae of Shichito strain showed a high degree of complement fixation in the antiserum of Shichito or Tanizawa strain.
Namely, when purified rickettsiae were used as the antigen of the complement fixation test, a significant inhibition of hemolysis was observed to a higher dilution of the antiserum of the homologous strain than that of the heterologous strain.抄録全体を表示PDF形式でダウンロード (731K) -
第1編 感染鼠肺抗原と,微粒子吸着抗原による実験難波 一彦1958 年 70 巻 1 号 p. 221-232
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーThe peritoneal exudate containing a large amount of rickettsiae were obtained by intraperitoneal inoculation of Rickettsia tsutsugamushi into the guinea-pigs previously injected with chlorpromazine. The antigenic substance of thus obtained peritoneal exudate was absorbed on bentonite and pine-resin particles, and was used as the agglutination antigen. The flocculation antigen was prepared from the lung of the mice intranasally inoculated with rickettsiae. By means of the seroreaction with the use of these two sorts of antigens, the author studied the influence of the difference of the amount of rickettsiae as well as the sites of inoculation on the immunity. Intracutaneous, subcutaneous, intracerebral, intraperitoneal and intranasal routes were chosen as the sites of inoculation of living rickettsiae.
The intracutaneous inoculation produced the flocculation antibodies in the largest amount, the intracerebral and subcutaneous ones the nest, and they were followed by the intraperitoneal and intranasal ones.
In the case of inoculation by the routes other than nose, the antibody titer rised with the lapse of time after inoculation.
When inoculated by the same route, within the range of a certain amount of rickettsiae, the difference of amount gave no influence on the production of antibodies.
The agglutination reaction with the use of the particle-absorbed antigen was not feasible because of its non-special reaction.抄録全体を表示PDF形式でダウンロード (861K) -
第2編 恙虫病病毒感染天竺鼠の腹水の補体結合反応原性と,感染防禦賦与能に関する研究難波 一彦1958 年 70 巻 1 号 p. 233-243
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーThe ascites of the guinea-pigs previously injected with chlorpromazine and then inoculated with Rickettsia tsutsugamushi was separated into two fractions, tissue and rickettsial fractions, from which formol vaccines were prepared. Each of these formol vaccines was subcutaneously injected into mice, and the relationship between infectious protecting power and complement-fixing antigenicity was investigated.
In regard to the immunity, the vaccine of tissue fraction was not inferior to that of rickettsial fraction, and the complement-fixing antigenicity of each revealed no marked difference. In either vaccine of these two fractions, the immunization by a singe injection of 0.1cc could hardly give an immunization effect against infection, and the sera of thus treated mice showed no complement fixation with the ascites of guinea-pigs containing a large amonnt of rickettsiae. The mice which received 2 times of injection of 0.3 cc vaccine showed a marked resistance to the attack of the homologous strain, and also a slight resistance to that of the heterologous strain. Furthermore, the complement fixation reaction using thus immunized sera and the ascites antigen of the homologous strain gave the antibody titer of 10-40×, while in the reaction with the ascites antigen of the heterologous strain the antibody titer was 10-20×. When immunized by 3 times of injection of 0.3 cc vaccine, the resistance against the attack of the homologous strain was marked and was moderate against that of the heterologous strain, and the complement fixation reaction with the sera of thus immunized mice yielded the antibody titer of 80-160× against the ascites antigen of the homologous strain and 20-160× against that of the heterologous strain.
When the sera of the mice immunized by the above-mentioned vaccines show the complement fixation titer of over 20× against the ascites antigen of the homologous strain, it may be assumed that the immunized mice possess a defensive power against the rickettsial infection.抄録全体を表示PDF形式でダウンロード (870K) -
第1編 1腹水病毒の蛋白分劃の性状について西本 忠治1958 年 70 巻 1 号 p. 245-257
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーThe antigen analysis of Rickettsia tsutsugamushi has not been studied as yet because of the difficulty in obtaining rickettsiae in a large amount. However, the author could obtain a large quantity of rickettsiae from the peritoneal exudate produced by intraperitoneal inoculation of R. tsutsugamushi into the guinea-pigs previously injected with chlorpromazine. Mitani and Tanizawa strains of R. tsutsugamushi were chosen as the test agents.
After homogenizing the exudate for 1 minute, the tissue fraction was separated from it by centrifugation at 3000 rpm for 30 minutes. By salting out of the supernatant of exudate with ammonium sulfate, the albumin and globulin fractions were obtained, and, in addition to these, the polysaccharide fraction was obtained by removing protein with trichloroacetic acid. Each fraction was dialyzed, corrected to the former volume, and then its pH was adjusted to 7.2.
Each of the albumin, globulin, polysacchride and tissue fractions did not show any hemolysis in sheep blood cell suspension.
In regard to the total protein content, the albumin-globulin ratio, both of which were measured by paper electrophoresis, and LD50 in mice, no significant difference was observed between the peritoneal exudate containing visible rickettsiae and that not containing them; this result means that the observation of rickettsiae by Gimsa's staining cannot give a exact and definite determination of the infection of the disease.抄録全体を表示PDF形式でダウンロード (1851K) -
第2編 2.腹水病毒の蛋白分劃の補体結合反応原性について西本 忠治1958 年 70 巻 1 号 p. 259-266
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーIn the present report, the author investigated the complement-fixing antigenicity of the albumin, globulin, polysaccharide and tissue fractions of the peritoneal exudate of guinea-pigs infected with Rickettsia tsutsugamushi. Mitani and Tanizawa strains of R. tsutsugamshi were used as the test agents.
Each fraction was obtained, in such a way as mentioned in report I, from the peritoneal exudate of guinea-pigs previously injected with chlorpromazine and then inoculated with rickettsiae. The antisera were obtained from the rabbits immunized by inoculation of rickettsiae into the vein.
Against each of the antisera of Mitani and Tanizawa strains, both of albumin and tissue fractions showed a high antigenicity, while globulin and polysaccharide fractions showed no noticeable antigenicity.
From this result, it is concluded that albumin and tissue fractions play a chief role in the complement-fixing antigenicity of the peritoneal exudate of rickettsia-infected guinea-pigs.抄録全体を表示PDF形式でダウンロード (504K) -
第1編 恙虫病病毒感染ハツカネズミの抗体産生に及ぼす抗生物質等の影響に関する実験三井 正雄1958 年 70 巻 1 号 p. 267-275
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーIn respect to the dose of antibiotics and the period of administration, the author studied the influence of antibiotics on antibody production in mice infected with Rickettsia tsustugamushi.
Chloramphenicol was used as the test antibiotic, and the antibody production was estimated by the Weil-Felix reaction and cellular figure of the ascites. The results were as follows:
1) When chloramphenicol was given to mice in the begginning period, 24 hours after inoculation of R. tsutsugamshi, the antisera of mice showed a positive agglutination reaction to 20× to 40× dilution.
2) When chloramphenicol was given to mice in the later period, 8 days after inoculation of R. tsutsugamushi, the antisera of mice showed a positive agglutination reaction to 80× to 120× dilution; a almost similar result in the control group of mice not given chloramphenicol,
3) The agglutination titer of antisera was a little higher in the mice continuously administered small doses of chloramphenicol than in those administered once a big dose.
4) In the group of mice given chloramphenicol in the begginning period, the cellular examination of the ascites revealed the increase of histiocytes and monocytes, and the slight decrese of serous cells and lymphocytes. In those given chloramphenicol in the later period, the increase of serous cells, lymphocytes as well as histiocytes was observed; nearly the similar cellular figure to those in mice receiving no chloramphenicol administration.
From these results, it is considered that the administration of chloramphenicol has a very great influence on the production of antibody.抄録全体を表示PDF形式でダウンロード (654K) -
第2編 恙虫病生・死病毒の免疫原性に関する実験三井 正雄1958 年 70 巻 1 号 p. 277-291
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーMice were inoculated with living and inactivated vaccines of Rickettsia tsutsugamushi by various different routs, intraperitoneal, intracutaneous, subcutaneous, intracerebral and intratracheal. The grade of immunity of thus treated mice was studied in respect to the sorts of vaccines and the routs of inoculation. Mitani and Tanizawa strains of Rickettsia tsutsugamushi were used as the test agents. The results were as follows:
1) Irrespective of the routes of inoculation and life or death of vaccines, the production of antiboies in mice remained almost independent of the sorts of rickettsial strains.
2) In the case of inoculation of living vaccines, the production of antibodies was the best in those inoculated by intracutaneous route and decreased in the sequence as follows; subcutaneous, intraperitoneal, intracerebral and intratracheal.
3) In the case of inoculation of inactivated vaccines, the inoculation by intraperitoneal route produced the antibodies in the largest amount, and the antibody production decreased in the following order; intracutaneous, subcutaneous, intracerebral and intratracheal.
Namely, the production of antibodies in mice largely depends on the nature of vaccines as well as the inoculation routes. Regardless of the nature of vaccines, however, intraperitoneal inoculation seems to produce the highest immunity.抄録全体を表示PDF形式でダウンロード (1111K) -
第1編 治療方法並びに治療成績武内 元成1958 年 70 巻 1 号 p. 293-301
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーThe author studied hearing acuity, findings of nsopharyngoscopic examinations and changes in the patency of the Eustachian tube in the 277 ears of the children with tubal stenosis, ranging in age from 5 to 15 years, who had received various tseatments without success in our Better Hearing Clinic, Departmment of Oto-Rhino-Laryngology, Okayama University Medical School and who has been finally submitted to the radium irradiation therapy
1) As for the method of radium irradiation, a piece of radium needle containing 5.5 mg. radium is put in a brass holder 1 mm. thick and it is inserted up to the orifice of the Eustachian tube through the nose and fixed in the nasopharyngeal cavity. As for the amount of irradiation, an individual case receives 3 to 4 times of 33 mg. hour of irradiation for six hours at a time at the interval of seven days, which is considered as a round of teeatment. Usually only one round of such treatment has been given, but in some cases two or three rounds have been administered with 2 to 5 months' interval between the rounds.
2) Out of the 216 ears examined by an an audiometer 144 ears (66.7%) have recovered to the normal hearing; and the 61 ears given infant speech tests have likewise yielded approximately the same results.
3) In order to obtain a better result in the radium treatment, it is necessary to give first such treatments as the treatment for the fluid in the middle-ear cavity, adenoid, or sinusitis.
4) Out of 156 ears, 104 (60, 6%) improed by the irradiation treatment. In these 104 cases the majority of them showed granular change of the Eustachian orifice or residual adenoid in the nasopharynx, and furthermore, these types were the ones to whom the irradiation treatment proved to be most effective.
5) As for the patency of the Eustachian tube, 63 ears (60%) of 105 cases with severe stenosis showed improvement after the irradiation, while 38 ears (80%) of 47 cases with mild stenosis improved,
6) As the relapse is apt to occur even more than one year after the treatment, it is necessary to carry on the periodical follow-up examinations.抄録全体を表示PDF形式でダウンロード (774K) -
第2編 遠隔成績武内 元成1958 年 70 巻 1 号 p. 303-309
発行日: 1958/01/31
公開日: 2009/03/30
ジャーナル フリーBy carrying out follow-up examinations for the period ranging from two to six years after the radium therapy, the author obtained the following results.
1) In the examination around the nasopharyngeal regions by nasopharyngoscopy no scar formation could be recognized even in the cases given two rounds of the treatment and likewise no subjective complaint as a side-effect such as feeling of driness was revealed.
2) During the course of follow-up examinations there occurred 22 relapses (28.6%) out of the 77 ears. In the majority the relapse occurred generally within six months after the treatment, but in some it occurred after more than six months to one and a half years afterward.
3) As for the findings concerning the conditons of the auditory tube in the examinations either by nasopharyngoscopy or of the patency of the Eustachian tube, all showed a marked improvement.
4) Concerning the hearing recovery at the time of re-examinations conducted during the periods from two to six years after the treatment, severe cases showed a morn marked improvement rather than the mild cases. Of them the majority revealed the average hearing loss of about 10 db in speech range. Furthermore, these re-examined cases revealed a better hearing recovery than the recovery obtained at two to six weeks after the treatment.抄録全体を表示PDF形式でダウンロード (2650K)
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