With a view to use in the tissue culture of Japanese B encephalitis virus, the author studied the fluid media, culture methods, and various cells appearing during the culture by performing the rollertube culture of the chick-embryo brain.
1) When the cerebellum of chick-embryo is cultured in the rollertube, the explant spreads out rapidly on the slide glass, making it quite suitable for the phase-contrast microscopic observations.
2) When the central nerve tissue of chick-embryo, fixed in plasma clot but after a previous stationary culture, is cultured in the rollertube, the tissue fall-out can be prevented.
3) As for the fluid medium in the tissue culture of chick-embryo brain the solution composed of 85 percent of B. S. S. (balanced salt solution) and 15 percent of C. E. E. (chick embryo extracts) is quite suitable, and it is also commendable for the virus culture.
4) Microscopic structure of wandering cells, particularly that of non-neuronal cells, has been studied with the phase-contrast microscope. In the explant of the chick-embryo brain, vitally stained, nerve cells have been observed wandering out actively.
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