岡山医学会雑誌
Online ISSN : 1882-4528
Print ISSN : 0030-1558
70 巻, 3supplement 号
選択された号の論文の7件中1~7を表示しています
  • 稲臣 成一, 木村 道也, 山口 昇, 水落 理, 大塚 信夫
    1958 年 70 巻 3supplement 号 p. 1-4
    発行日: 1958/03/20
    公開日: 2009/03/30
    ジャーナル フリー
    In 1957 mosquitoes were collected as usual, by means of light traps in Okayama, Kurashiki, Mizushima, Tamashima, Tamano, Kojima, Seto, Tsuyama and Niimi in April through October. From the results obtained the peak of population density for Culex pipiens was found in July, that for C. tritaeniorhynchus in August, while an endemic of the encephalitis broke out in early September.
    Inoculation tests made on mice with the mosquitoes collected in Okayama and Kurashiki areas, however, were negative.
  • 木村 道也
    1958 年 70 巻 3supplement 号 p. 5-7
    発行日: 1958/03/20
    公開日: 2009/03/30
    ジャーナル フリー
    Wenn Rattus norvegicus mit dem Virus von Encephalitis japonica durch Impfung in das Gehirn infiziert wird, so findet bei der Ratte während einer Periode von 72 bis zu 96 Stunden nach der Impfung eine Virämie statt, ohne dass irgend eine klinisches Zeichen auftritt. Es scheint dem Verfasser dass die Ratte in der Epidemiologie diesser Krankheit eine Rolle als ein vorübergehender Reservewirt spielt.
  • 浜 聡, 小野 安三, 松山 恒男, 伊達 嘉寛, 大亀 学
    1958 年 70 巻 3supplement 号 p. 9-14
    発行日: 1958/03/20
    公開日: 2009/03/30
    ジャーナル フリー
  • 第一篇 鶏胎脳組織懸滴培養に及ぼす日本脳炎ウイルスの影響
    出宮 美克
    1958 年 70 巻 3supplement 号 p. 15-21
    発行日: 1958/03/20
    公開日: 2009/03/30
    ジャーナル フリー
    As is well known embryonated eggs are used for the culture of all kinds of the virus. With the use of droplet culture the author in the present experiment conducted a series of experiments in order to study influences of the virus of Japanese B encephalitis, the virus which is neurotropic, on the central nervous system of the chick embryo.
    When the normal brain of chick embryo is cultured under a certain fixed condition, nerve fibers grow almost uniformly.
    When the Japanese B encephalitis virus is inoculated into the embryonated eggs, the eggs will die on the average of 5 days after the inoculation. However, judging the function of nerve cells by the growth rate of nerve fibers during the culture, it starts to decline on the 3rd day and on the fifth day, the day immediately before death, it is almost lost. Similarly the fnnction of other cells is lost, too. On the other hand, although there is a marked hyperemia in the pathohistological finding, no significant changes as might be expected of the tissue culture findings can be recognized in the nerve cells as well as in other cells. Namely, even where there is no apparent histological abnormalities, the cell functions are actually disturbed to a marked extent.
  • 第二篇 鶏胎脳組織の廻転培養並に出現する諸細胞に関する観察
    出宮 美克
    1958 年 70 巻 3supplement 号 p. 23-34
    発行日: 1958/03/20
    公開日: 2009/03/30
    ジャーナル フリー
    With a view to use in the tissue culture of Japanese B encephalitis virus, the author studied the fluid media, culture methods, and various cells appearing during the culture by performing the rollertube culture of the chick-embryo brain.
    1) When the cerebellum of chick-embryo is cultured in the rollertube, the explant spreads out rapidly on the slide glass, making it quite suitable for the phase-contrast microscopic observations.
    2) When the central nerve tissue of chick-embryo, fixed in plasma clot but after a previous stationary culture, is cultured in the rollertube, the tissue fall-out can be prevented.
    3) As for the fluid medium in the tissue culture of chick-embryo brain the solution composed of 85 percent of B. S. S. (balanced salt solution) and 15 percent of C. E. E. (chick embryo extracts) is quite suitable, and it is also commendable for the virus culture.
    4) Microscopic structure of wandering cells, particularly that of non-neuronal cells, has been studied with the phase-contrast microscope. In the explant of the chick-embryo brain, vitally stained, nerve cells have been observed wandering out actively.
  • 第三篇 日本脳炎ウイルスの鶏胎脳培養組織に於ける増殖及び細胞変性. (附)鶏胎心線維芽細胞に及ぼす日本脳炎ウイルスの影響に関する研究
    出宮 美克
    1958 年 70 巻 3supplement 号 p. 35-46
    発行日: 1958/03/20
    公開日: 2009/03/30
    ジャーナル フリー
    With the purpose to study the cytopathogenic effect of Japanese B encephalitis virus, the author performed the tissue culture of the chick-embryo brain in rollertube and inoculating the virus into the tissue. observed changes occurring in cells.
    1) In brain cells cytopathogenic effect appears at a certain fixed period of incubation after virus inoculation, but in cardiofibroblasts although growth is inhibited, no cytopathogenic effect can be detected.
    2) As for the phase contrast microscopic findings. contraction and distension of processes from various cells as well as the fragmentation of mitochondria can be observed; and in the stained specimens the shrinking of the nucleus and eosinophilic substance in the cell can be recognized.
    3) Infection titre of brain cells reaches its maximum of 10-5, 5 after 48 hours, and later it rapidly falls due to cytophatogenic effect. In the case of fibroblasts the maximum is 10-3 and this titre is maintained.
    4) By adding the serum of immunized guinea Pig. it, has been possible to inhibit the cytopathogenic effect which appears at a certain fixed period of incubation after inoculation of the virus.
  • 緒方 益雄, 大田原 一祥, 大平 昌彦, 奥田 久徳
    1958 年 70 巻 3supplement 号 p. 47-55
    発行日: 1958/03/20
    公開日: 2009/03/30
    ジャーナル フリー
    昭和32年岡山県下に於ける日本脳炎の流行を,時期的,地域的に又性,年令別に観察し若干の考察を加えた.昭和32年の流行は患者発生数113名,死亡者38名であつて,中程度の流行と見られ,流行の時期的推移はほぼ例年通りであるが,県南部の流行と県北部の流行に時期的に約2週間のずれがあるという所謂北進現象は明瞭でなかつた.地域的観察では県南部特に倉敷市に患者の多発を見,これ又従来の流行と同様の傾向である.性,年令別患者発生状況に於ては従来の流行に比し県北部に於ける,男子幼年層の患者が増加した傾向が認められた.致命率は全般には33.6%であつて,依然としてかなりの高率を示し,改善の徴は認められないようである.なお男子の致命率が女子の致命率よりも高い値を示したがこれは昭和21年以降の流行中,昭和29年に1度見られただけで,異例の現象と考えられる.流行衰退期に現われる現象かとも考えられるが今後の検討に待ちたい.
    日本脳炎予防接種は,相当有効であつて,罹患率をかなり低下させるものと認められ,今後更に広範囲に実施すべきものと考える.昭和32年の流行は中程度の流行であるが,流行の周期性から見れば,流行衰退期に当ると考えられ,又2, 3年後にかなりの大流行も予想され得るから,抜本的蚊の防除や予防接種等の防疫対策上考慮すべきであろう.
    昭和32年の流行に際し日本脳炎患者脳材料より分離された「岡山57G」株は日本脳炎中山株と同定され,抗原変異等は無いものと認められた.
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