1) An attempt was made to study on an anti-tumor agent, nitromin, based on its medication period against subcutaneously transplanted ascites hepatomas MH 134 to C3H/HeN (_??_) X dd (_??_): F1 mice. 2) The earlier administration of nitromin after the tumor transplantation would inhibit the tumor growth more effectively. When the administration was continued from the 3rd day after the transplantation, some cases could be found to survive more than a year. 3) The earlier nitromin administration would extend the survival period in 50 % the cases. The administration of nitromin that was continued from the 10th day after the transplantation can hardly inhibit the tumor growth; in contrast, the administration which was started within the 7th post-transplantation day appears to be effective to inhibit the tumor growth. 4) The superficial lymphnode metastases were observed in cases of the long survived mice, that is, those which received the anti-tumor agent in early stage. It does not mean the ineffectiveness of the agent, but the result of the long survival with the agent. 5) The lung metastasis tends to occur in cases of the late administration of the agent.
1) This experiment was carried out to study the combined therapy of anti-tumor agent with surgical operation, especially for the analysis of its administration period. The ascites hepatomas MH 134 was subcutaneously transplanted to the mice, C3H/HeN (_??_) X dd (_??_): F1 hybrids, and nitromin was administered. 2) According to the survival rate in cases of the combined therapy of the agent with radical surgical operation, the preoperative administration appeared to be more effective than the postoperative. The survival rate is higher in the group with preoperative administration. 3) Death coused directly by tumor growth occurred in most of the cases within 90 days after tumor transplantation. There was hardly found any difference between the pre- and postoperative administrations. 4) The combined therapy seems to be apparent to increase the survival rate, compared with the non-treated group. 5) Some animals have metastases in lymphnodes, lung, liver and other organs without local recurrence in the group with postoperative combined therapy, while in that with preoperative combined therapy there was never seen a mode of such metastasis, but both of the local recurrence as well as metastases to those organs were observed. It seems to be due to the tumor cells in circulating blood at the time of surgical manipulation. It is therefore apparent that the preoperatively administered anti-tnmor agent can inhibit the metastasis formation but the postoperative cannot.
1) This study was made to find out the possibility of metastasis of tumor cells via circulating blood and also the effect of anti-tumor agent to the cells transfered through blood. Nitromin was used as an agent. The blood of the C3H/HeN (_??_) X dd (_??_): F1 mice subcutaneously bearing ascites hepatomas MH 134 was subcutaneously given to the tail of another group of intact F1 mice. 2) The metastasis was appeared to be possible without any relation of the time interval between the tumor transplantation to the donors and the blood injection from the donors to the recipients. Transplantation was not successful when the anti-tumor agent was given within the interval. 3) The survival time seems to be kept constant in cases of successful transplantation. 4) Transplantation was more successful from cases bearing large tumors and surviving for longer periods, and from cases bearing small tumor and surviving for shorter period. 5) The same is true in weight of the tumors. 6) The spleen of the tumor-bearing mice weighed more than that of the non-bearing mice. The group treated with chemotherapy tends to have less weighing spleen in contrast to the non-treated group. It is interesting to note that the tumor-bearing mice whose blood could form metastas_??_s in another mouse appeared to have the heaviest spleen.
The author des ribes the results obtained in the series of tissue culture in fluid medium using the bone marrow from normal persons and patients with various blood diseases as materials supplemented with iron or vitamin B12, in order to observe the alteration in the number of sideroblasts, and discusses about the iron metabolism of erythroblasts. 1 In the bone-marrow tissue culture of normal persons the iron addition to the culture slightly increases the number of sideroblasts. 2. In the cases of bone-marrow tissue culture of idiopathic hypochromic anemia, hook worm anemia, Banti's disease and malignant tumor, sidereblasts are increased considerably in proportion to the degree of iron deficiency state in these diseases by the iron addition. 3. In the cases of hypoplastic anemia and liver cirrhosis, sideroblasts are hardly or only slighly increased. 4. In the cases of leukemias the incresse in the number of sideroblasts in vitro by the addition of iron differs widely from a considerable increase to a very small incease. 5. Despite the fact that polycythemia vera shows a low rate of sideroblast appearance, there can berecognized no appreciable increase in the number of sideroblasts by the addition of iron. 6. In the cases of iron deficiency and the lowered or accelerated functions of bone-marrow, each of them reveals certain different patterns of alteration in the number of sideroblasts and to this phenomenon stainable iron of erythroblasts before addition plays an important role. 7. However, in the cases of the bone-marrow tissue culture supplemented with vitamin B12 sideroblasts are hardly affected, and therefore, vitamin B12 has almost no effect on the iron metabolism of erythroblasts.
By adding iron or cobalt to the tissne culture in fluid medium of bone marrow from normal and various experimental anemic rabbits the author has observed the alteration in the number of sideroblasts and studied the iron metabolism of erythroblasts. The following are the results. 1. In the case of the bone-marrow tissue culture of normal rabbit supplemented with iron sideroblasts are slightly increased in number. 2. In the case of bensol or collargol anemia hardly any increase can be recognized in sideroblasts by the iron addition. 3. In the case of the rabbit irradiated with 300 r. the addition of iron increases sideroblasts in number considerably. 4. In the case of hemorrhagic anemia the addition of iron markedly increases the number of sideroblasts as the iron deficiency state advances in its degree. 5. Hardly any increase in the sideroblast number can be observed in the case of phenylhydrazine anemia supplemented with iron. 6. From these alterations in siderodlasts by the effect of iron addition, it is assumed that non-hemoglobin iron in erythroblasts has an important bearing on the iron uptake of erythroblasts. 7. Cobalt has an action as to direct the iron in erythroblasts towards the heme synthesis because in the case supplemented with iron plus cobalt the increase in the number of sideroblasts is smaller than that supplemented with iron alone.
By adding sera of the patients with various blood diseases along with iron to the bone-marrow tissue culture in fluid medium the author has observed direct influences of the sera on the erythrocyte series in bone marrow and studied their action from the standpoint of the iron metabolism in erythroblasts, and obtained the following results. 1. In the cases of the bone-marrow tissue culture supplemented with sera from idiopathic hypochromic anemia, Bant 's disease, Hodgkin's disease and a portion of leukemias (myelogenous leukemia, acute lymphocytic leukemia) along with iron, there can be recognized no factor that affects the iron metabolism of erythroblasts and hence these sera seem to have no direct action to inhibit the bone-marrow function. 2. It has been found that there is a factor that disturbs the bonemarrow erythropoiesis in the serum of hypoplastic anemia. 3. In the sara of cancer and monocytic leukemia patients likewise there is a factor that suppresses the erythropoiesis of bone marrow. 4. It is obvious that the serum of polycythemia vera contains a factor that accelerates the erythropoiesis of bone marrow.
For the purpose of finding out a suitable method of determining conditions and progress of lung tuberculosis, C-reactive protein tests were conducted 359 times on 157 cases of unoperated lung tuberculosis patients and 202 times on 33 cases of operated patients, and the following results were obtained. C-reactive protein is the substance first discovered by Tillet and Francis (1930) and it is not found in the serum of normal persons, but it appears often in the sera of such patients as in the cases of bacterial infection diseases and in those diseases which bring about an extensive tissue damage. Incidence of the cases positive to C-reactive protein (CRP) test is 49.1 per cent in those showing accelerated erythrocyte sedimentation rate, which is higher than that of 15.1 per ceut in those with normal erythrocyte sedimentation rate (ESR). It has been found that the higher is the ESR the greater is the positive rate of CRP. In those whose CRP is over one plus the ESR is 90-100 per cent, being extremely high. CRP in the casos of leukocytosis is 62.1 per cent, while in others it is 35.5 per cent. Classified according to leukocytes, the positive rate of CRP is 80 per cent in those with nucleus left shift, 63.1 per cent in those with neutrophilia, 100 per cent in those with eosinopenia, and 63.2 per cent in lymphocytopenia, all being higher than the other cases. The positive rate of CRP in febrile patients is 70.3 percent, higher than 32 per cent in afebrile patients. It is 51.4 per cent in Gaffky positive cases and 24.8 per cent in Gaffky negative cases, proving that the positive rate of CRP is high in open tuberculosis. In addition, the percentage of Gaffky positive cases in those with CRP of over 2 plus is about 80 per cent. The positiye rate of CRP in the tubsrculosis patients with cavity is 44.8 per cent, and it is 19.6 per cent in those without cavity, proving a higher rate generally in the patients with cavity. However, it is lower in those with isolated cavities, and the positive rate of CRP is dependent upon the nature and number of cavities as well as upon the pathological cha ges surrounding the cavity rather than presence or absence of the tu berculous cavity. Althongh the positive rate in these without the avity or with isolated cavities is 0-19.8 per cent, it ranges from 58.3 to 33.3 per cent in those with multiple chambered cavity or with the cavity located in an extensive pathologic focus, and it is 63.7 per cent in the cases with cavity belo ging to far advanced and combined type of lung tuberculosis. The positive rate CRP is high in severe lung tuberculosis and in extonsive exudative tuberculosis having strong activity; i.e. according to the lung tuberculosis classification by the Laboratorv of Department of Education, Japan, the positive rate is 63.7 per cent in F type (severe comdined type), 52.6 per cent in A type (exudative type), 31.0 per cent in C type (fibrous caseous type), 7.7 per cent in B type (infiltrative caseous type), and 0 per cent in D type (sclerotic type). Furthermore, according to N. T. A. classification, it is 6.4 per cent in minimal case of tuberculosis, 24.1 per cent in moderately advanced case, and 63 per cent in far advanced case. In the observations of CRP according to the progress of symptoms, about one half of the patients whose negative CRP turned positive shows aggravation of tuberculosis, and about one half of those whose positive CRP turned negative shows alleviation of the disease. In addition, almost all those with improved conditions tend to be negative, while thile those whose conditions worsened almost all tend to be CRP positive. All those who died proved to be CRP positive. As the CRP tends to turn positive in the case with other complication, in pursuing CRP along with the progress of tubercnlosis, it is necessary fo pay a close attention to preseuce or absence of complication that turns CRP positive.
Guinea pigs were divided into the next four groups for the purpose of examining the effects of exhaustive physical exercise and fatigue after exposing to X-ray. (1) Non treated group (C) (2) Exercised control group (EC) (3) Radiated control group (RC) (4) Radiated exercised group (RE) The X-ray apparatus was operated at 200 KV, 15 mA, 0.5 mm Copper and 0.5 mm Alminium filtration, 50 cm focus target distance. The dose was 200 r of X-ray whole body irradiation in dose rate of 80.6 r/min (in the air). Subjects of this experiment were given 30 successive days of swimming at the rate of 30 minutes per day and 5 days per week. The results are as follows: (1) The 30 days survival rate of RE was lower than that of RC. (2) The decreasing rate of the body weight in RE was a little higher than that in RC. (3) The prolongation of tbe blood clotting time in RE was greater than that in RC. (4) The decreasing rate of red cell count in RE was a little higher than that in RC. In both groups the decreasing rate was the highest on the 14th day after the treatment. (5) The decreasing rate of hemoglobin content in RE was a little higher than that in RC. In both groups the decreasing rate was the highest on the 14th day after the treatment. (6) Reticulocyte count in RE and RC decreased in the beginning, but increased extremely on the 21st day after treatment. Its increasing and decrasing rate in RE was little different from that in RC. (7) The increasing rate of Heinz body in RE was higher than that in RC. (8) The decresing rate of white cell count in RE was not significantly different from that in RC. In both groups the white cell count was at minimum on the 14th day after treatment. (9) Concerning the classification of white cell, i.e. basophile-, eosinophile-, neutrophile leucocyte, lymphocyte and monocyte, RE was not significantly different from RC. (10) About the changes of plasma proteins, a) The total protein in RC decreased a little in the beginning, but slightly increased in the latter period. That in RE showed little change in the beginning, and slightly increased in the latter period. Difference berween RE and RC was not significant. b) A/G ratio did not change much in the beginning in RC as well as RE, but in the latter period it decreased in RC while increased in RE. c) Albumin didn't show much change in the beginning both in RC and RE, but it decreased a little in the latter period where as in RC it increased in RE. d) α-globulin in RC and RE increased a little after the treatment. Difference between RC and RE was not significant. e) β-globulin in RC decreased a little in the beginning, but increased in the latter period. On the contrary in RE, it decreased extremely in the latter period. f) Fibrinogen in RC and RE increased. Difference between RC and RE was not significant. g) γ-globulin n RC and RE decreased in the beginning. That in RC increased extremely, while in RE continued to decrease in the latter period.
C57-Black female mi e were divided into the next 7 groups for the purpose of examining the effects of the exhaustive physical exer ise before and after the irradiation. Group 1. Exercised for 30 minutes directly before the irradiation (RE pre-30 m.) Group 2. Exercised for 1 hour directly before the irradiation (RE pre-1h) Group 3. Exercised for 2 hours directly before the irra iat on (RE pre-2h) Group 4. Exercised for 1 hour after the irradiation (RE post) This exercise was continued for 30 days at the rate of 1 hour per day and 6 days per week after irradiation. Group 5. Exercised control (EC) This exercise was the similar as that in Group 4 Group 6. Radiated control (RC) Group 7. “Thyradin” injected (TR) “Thyradin” (thyroid preparation) was injected 0.2 c. c. (contains 10 γ of iodine) per 10 grams of body weight, and then the animals were exposed to X-ray on the 7th day after irradiation. Exercised groups were made to run in the cylindrical tread-mill (80 cm in circumference, 8 rotations per minute, i.e. the animals have to run at a speed of 6.4 m per minute). Irradiated groups received 600 r, 660 r aud 840 r of X-ray at 99 r per minute. Gas analyses were done in order to evaluate the metabolic level of the exercise on one mouse in each group which was made to run in another tread-mill of the same diameter, rotating in the same speed as one described above, and located in an air-tight chamber shown in fig. 1. The air sample from this chamber was analysed with Scholander's apparatus. This results are as follows: 1) The metabolism increased by the exercise showing 33.85% (mean) increased of the oxygen consumption. 2) The metabolism was accelerated gradually by the “Thyradin” treatment arriving the maximum arround the 6th day after the injection, showing 42.99% of the increase of oxygen consumption. 3) The survival rate in the groups which were exercised before the irradiation was higher than that in the group of radiated control and the group exercised after the irradiation. The difference of the survival rate detween the groups exercised before the irradiation and the groups exercised after the irradiation was significant (P<0.05). 4) The survival rate in the groups which were exercised before the irradiation was not in proportion to the quantity of the exercise before the irradiation. 5) The metabolism was increased by the injection of “Thyradin” and lowered the survival rate. 6) The weight in the gronp of radiated control dropped down day by day, but in the group exercised before the irradiation began to recover after three weeks of irradiation. 7) The weight in the group in ected “T yradin” decrease extremely 2 weeks after the irradiation.
Phagocytosis of leucocytes in the blood of acatalasemia for carbon particles was investigated by means of the slide method, using the blood of healthy people and various oto-rhino-laryngological patients as controls. Phagccytosis in neutrophils was classified into five degrees from negative to 3 positives (-, ±, +, ++, +++), giving them values of 0, 1, 2, 3 and 4 respectively, according to the degrees of phagocytosis. The mean values counted by this method were as follows; 1) Number of healthy people examined was seven and the mean value was 2.55. 2) Nineteen otorhinolaryngological patients were examined and their mean value proved to be 2.50. The mean value of thirteen out of these nineteen patients was 2.54, except six cancer patients. Three out of these six cancer patients had been treated with radium and the mean value did not differ from that of healthy people, while three X-ray treated patients showed the mean value of 2.31. 3) Number of acatalasemia patients was two and their mean value 2.53. As far as the present experiment is concerned, there appears no significant difference in the degree of phagocytosis in leucocytes for carbon particles in the groups examined except X-ray treated cancer patient group, who showed lower mean value.
1) Fifty-three patients of good risk, aged between 24 and 64 years were utilized for this study. An analysis was made on respiratory-rate, -volume, tidal volume, expiratory CO2-content and blood CO2-content at F-O anaesthesia which was carried out in various mathodes; with 2% F-O for 25 minutes, or with 2% F-O for 5, 10 and 15 minutes followed by 1% F-O. One-half mg. of atropine was tried in some cases as preoperative medication. 2) In both cases of 2% F-O alone and 2% F-O followed by 1% F-O inhalation, an increased respiratory rate and, in contrast, a decrement of respiratory volume and markedly of tidal volume were observed. 3) Preoperative medication of atropine decreased such tendencies in grade compared with non-atropine group. It seems to be apparent that atropine preoperative medication acts against respiratory inhibition of Fluothane. 4) The changes of respiratory rate, -volume and tidal volume under 2% F-O anaesthesia can be slightly recovered at the switch over to 1% F-O anaesthesia. which is more noticed when atropine was used. The tidal volume, however, can not be recovered above the physiologically minimum level in any case. 5) The CO2 content in expiratory air was observed to be increased as the anaesthesia was deepened, which was, to some extent, inhibited by atropine. The content in the end tidal air, however, always increased above the physiological level in all cases. 6) Although the blood CO2 content was also increased by F-O anaesthesia, it was a little over the physiological limite of the variation. 7) Bennett assistor markedly inhibited the CO2 contents both in expiratory air and in blood.
1) Eighty four patients aged between 24 and 64 years were utilized for this study with 2% F-O inhalation for 25 minutes or with 2% F-O for 5, 10 and 15 minutes followed by 1% F-O. The maximam B. P., pulse rate, and pulse pressure were analysed. Atropine was used in some cases as a preoperative medication. Another group of 15 patients, having been inserted a tracheal tube under consciousness, was checked on cardiac-output, -index, stroke volume, circulatory blood volume and total peripheral resistance both before and 15 minutes after 2% F-O anaesthesia. Bennett assistor was used in some cases. EKG observation was also done. 2) In case of 2% F-O anaesthesia. an immediate decrement of BP was found in value of about 40 mm Hg. Atropine seemed to be little effective against the decrement. 3) A recovery of the BP decrement was observed when 2% F-O was replaced by 1% F-O. 4) Although bradycardia was observed during the anaesthesia of both 1% and 2% F-O, atropine could absolutely inhibit it. 5) The decreased pulse pressure at F-O anaesthesia is apt to be furthermore intensified by preoperative medication of atropine. This is one of the criteria against the regular routine preoperative medication of atropine and to suggest that the si e-effects of F-O anaesthesia in circulation and respiration should be taken account under the base of controling the F-O concentration. 6) It seems to be apparent that the BP drop by F-O inhalation is mainly due to the central inhibition of vasomotor system with the secondary dilatation of peripheral vessels, in addition to the inhibiting mechanism of cardiac muscle which causes the decrement of cardiac output. 7) EKG obsevations reveal a vagotonic state with nodal rhythm at F-O anaesthesia in most cases. Ischemia of cardiac muscle manifesting flattening or invertion of T-wave can not be observed. The improvement of preoperative EKG would rather be found in a number of cases.
Technique: An attempt was made to study a correlation of EEG, clinical anaesthetic depth and Fluothane concentration in blood by the Robson's method at Fluothane-Oxygen anaesthesia. Fourty-two adult human cases of both sexes, having no abnormal manifestations on EEG in awake state, were utilized for this analysis. Demerol, scopolamine and pentobarbital calcium were given in each case as a preoperative medication. Two % of F-O inhalation with semiclos d method was introduced for 25 minutes through a tracheal tube which has been inserted under consciousn ss by means of sup rior laryngeal nerve and translaryngeal blocking with 2% xylocaine. 1) No EEG variation was observed in any case of the preoperative medication. 2) During F-O an esthesia, however, a characteristic EEG patterns were found; increased amplitude and decreased frequency as the anaesthetic depth deepens. But slow waves were not detected until he depth becomes th 3rd plane of the III stage or without hypercarbia. 3) The Fluothane concentration in blood appeares to be increased which the anaesthetic depth is deepened. 4) It seems to be concluded that both EEG manifestations and Fluothane concentration in blood maintain a parallel relation with the depth of F-O anesthesia. This is illustrated as follows;
By observing the histological changes in the brains after administering ACTH to monkeys and mice with experimental Japanese encephalitis, the author studied the effects of ACTH on pathological changes of the brain, and arrived at the following conclusions. 1. In the case of the mice, it has been found that ACTH tends to diminish inflammatory changes, and it markedly lessens the diffuse proliferation of glia cells. 2. The inhibitory effect on the diffuse proliferation of glia cells shows no parallel relationship with the dosage of ACTH administered. 3. In the case of the monkey with the ACTH administration, there can be observed a similar tendency to lessen the imflammatory changes and in addition, the demolition of nerve cells is inhibited. 4. With these findings as the basis, some of the therapeutic effects of ACTH on Japanse encephalitis have been discussed.
After administering ACTH to m nkeys and mice with experimental Japanese encephalitis the author observed histological changes in the adrenal cortex, and obtained the following results. 1. In the case of the mouse adrenal cortex, Sudan stainable granules, Schiffsreaction; Schultzés reaction and double refractive substances tend to show a slight decrease and grow finer by the 48th hour after the inoculation of encephalitis virus, and these decrease markedly by the 72nd hour later, revealing congestion in all layers of the adrenal cortex. By the 96 th hour there can be recognized the disappearance of Sudan stainable substances. 2. In the case of the mi e with experimental encephalitis administered with 1 mg ACTH, Sudan stainable granules are markedly decreased already by the 48 th hour, leading them to death per se. 3. In the animals given 0.1 mg or 0.01 mg ACTH there can be recognized a decrease in the size and quantity of Sudan stainable granules by the 48 th hour, but none of them show the disappearance of this substance, pr ving that ACTH at such a small dose delays the decrease in Sudan stainable substance. 4. In weighing the mouse adrenals of each group there can be recognized no uniformity in weight. 5. In a monkey not treated with ACTH and progressed acutely, hardly any histological changes can be observed in the adrenal cortex. In others which lifespan was over 6 days, treated with or without ACTH, there can be recognized no clear-cut difference in the adrenal cortices. 6. From these findings it is assumed that the adminstration of ACTH to the animals with experimental Japanse encepealitis does not increase the stress on the adrenal cortex as to debilitate the animal but it rather acts protectively on the fnnction of the adrenal cortex. 7. As the administration of ACTH has an important bearing on the function of the adrenal cortext, it is advisable to pay a close attention to the fact that an excessive dose of ACTH will have a danger of bringing about undesirable effect.
In the histological observations of the hypophsis conducted for the purpose to find out pathological changes in the hypophysis of the mice and monkeys with experimental Japanese encephalitis, treated with ACTH, the author obtained the following results. 1. In the case of the mice with Japanese encephalitis, the number of α cells is increased with the progress of the disease and also there can be observed an increase in the number of typical α cells rich in α granules and with abundant cytoplasma. 2. As for β cells, although there can be re ognized no change in the rate of their appearance, there is an increase in the number of cells with regressed cytplasma with few granules. 3. The incidence of appearance of principal cells is decreased. 4. In the mice with Japansese encehpalitis treated with ACTH, in those mice given 1 mg ACTH the number of α cells rather tends to decrease, and in the group treated with 0.01 mg ACTH there is an increase of α cells in about the same proportion as in the untreated group. 5. In the case of monkeys α cells are increased in number due to Japanese encephalitis just as in the case of the miss with encephalitis, but there can be recognized no distinct difference between the one treated with ACTH and the untreated one, with respect to the number of α cells. 6. From these findings it is reasonable to assume that the cells that produce ACTH are α cells. 7. In the histological observations of the anterior lobe of the hypophysis of the mice with experimental Japanse encephalitis there can be found no inflammatory changes such as congestion and cell infiltration, while in the case of the monkeys with Japenses encephalitis there can be observed congestion and hemorrhage but neither infiltration of cells nor proliferation of interstitium can be seen.
The perfusion of the cat brain was performed according to the method of Ikeda, H. who modified the method of Geiger, A.. Electrocorticogram, cerebral blood flow, and biochemical and pharmacological findings in the case of the administration of GABOB were obtained respectively by the use of the perfusion method, and then the correlation of each appearance waseinvestigated. 1). Electrocorticogram of perfused cat brain was supressed by administration of GABOB in all cases. 2). The rate of cerebral blood flow, oxygen and glucose consumption and the liberation of lactic acid were all increased by the same treatment. 3). The above mentioned changes were reversible, namely those turned to normal soon after the interruption of GABOB administration. 4). All reflexfunctions as conjunctival reflexes, corneal reflexes, pupillary reactions and pain sensations, and respiration rate, blood pressure, electrocardiogram had represented no remarkable change. Accordingly, any activating effects of GABOB on ehe function of the perfused cat brain was not observed in all experiments.
The perfusion of the cat brain was performed according to the method of Ikeda, H. who modified the method of Geiger, A.. Electrocorticographical, biochemical and pharmacological findings in the case of the administration of β-OHGA were obtained, and then the correlation of their appearances were investigated. 1). Electrocorticogram of the perfused cat brain was activated by the administration of β-OH-GA. 2). Following the activation of electrocorticogram, all reflexes as conjuctival, corneal reflexes, pupillary reactions and pain sensation were activated, and then spontanous body movement appeared at the same time. 3). Cerebral blood flow rate, oxygen and glcose consumption, lactic acid liberation were all inereased. 4). The changes as above were reversible and soon recovered again by the perfusion without β-OH-GA. 5). Electrocorticogram and respiration rate did not showed any alteration. And, changes of blood pressure showed different case by case and represented not determinated tendency. 6). Any significant difference between effects of threo-type and erythro-type of β-OH-GA on the perfused cat brain was not observed.
For examining the blood group and precipitin reaction of the dried blood or saliva stain, the author used the heat and mechanical actions under ultrasonic radiation. The results are briefly as follows: It was apparent that the de erminations of blood type and precipitin reaction were rapidly completed by ultrasonic radiation (710 kc) of intensity of 1000 V (radiation time-over 10 minutes) or 1500 V (radiation time-over 5 minutes) against the dried blood stain and 1000 V or 1500 V (radiation time-both over 5 minutes) against the dried saliva stain.