岡山医学会雑誌
Online ISSN : 1882-4528
Print ISSN : 0030-1558
76 巻, 1-3 号
選択された号の論文の15件中1~15を表示しています
  • 第一報 標準株(K株)の性状について
    浜崎 充彦
    1964 年 76 巻 1-3 号 p. 1-11
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    Ehrlich Ascites Tumor Cells (JTC-11) reported here have been cultured in vitro for more than 3 years by pipetting, using 80% YLE+20% Bovine serum (final concentration: yeast extract 0.08%, lactalbumin hydrolysate 0.4%) as the medium. As the results of tissue culture experiments concerning the characteristics of this strain, I obtained the following conclusion.
    1) This (JTC-11) K-Strain is the easiest group of acutual animal malignant tissue culture strains in points of cell preservation, culture-method (pipetting), stability, medium-unchanging during one subculture term, inoculum size and adhesion on the glass surface.
    2) The proliferation rate is the greatest (about 2000 times for 8 days) and even a small number of cells have the ability of proliferation.
    3) After the intraperitoneal reinoculation into female mice with more than 1.6×104 this strain cells, all the mice died by the tumor. Accordingly tumor producing capacity has been strongly preserved after a lapse of 3 years.
    4) The strain-character (Sato) of this K-strain cells has still remained after in vivo 100 days transplantion through Cb-mice (Experiments of retissue-culture).
    5) This K-strain cells have a interesting form, namely the small sucker-form podia appear at the tip of the processes.
  • 浦上 博之
    1964 年 76 巻 1-3 号 p. 13-19
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    The mechanism of X-ray irradiation on the function and structure of mitochondria was studied by analysis of mitochondrial swelling-shrinkage, oxidative phosphorylation, Fe ions induced swelling, and Fe ions induced lipid peroxidation. Mitochondria were isolated from the livers of mice at 3, 6, 12, 24 and 48 hours after whole body irradiated 500 r with X-ray, and from non-irradiated mice. The results of the experiment are as follow:
    1. The activity of oxidative phosphorylation of mitochondria was reduced by 50 per cent after irradiation for 6 hours and returned to normal after 24 hours.
    2. Respiratory control of mitochondria was decreased by 40 per cent after irradiation for 6 hours and remained at this low level for 48 hours.
    3. The degree of swelling-shrinkage of mitochondria (about 20 per cent changes in volume) measured by 90° light scattering was reduced to some extent during 6 to 12 hours after irradiation and then recovered to control level after 24 hours.
    4. The large amount of mitochondria to show the certain extent of 90° light scattering was required at 6 hours after X-irradiation and returned to normal after 48 hours.
    5. Swelling, measured by the absorbancy at 520 mμ, and lipid peroxidation of mitochondria induced by Fe ions were increased in there initial velocity and in there extent by X-irradiation. These increments were maximum at 12 hours.
    6. These results suggest that the increment of peroxidizable lipid of mitochondria is a fact for the uncoupling of oxidative phosphorylation with X-irradiation,
    The possible role of uncoupling of oxidative phosphorylation by X-irradiation has been discussed.
  • 平松 直
    1964 年 76 巻 1-3 号 p. 21-39
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    老年期精神障害の臨床的研究を行い次の結果を得た.
    1) 60才以上の入院患者244名中,進行麻痺,アルコール中毒症等と診断されたものを除く135名を主要症候より分類した結果,痴呆群23名(17%)躁うつ群25名(19%)妄想群23名(17%)神経症群14名(10%)錯乱譫妄群14名(10%)脳動脈硬化症群36名(27%)がみられた.
    2) 発病年令より分類してみると, 188名中60才以前に発病したものは53名(28%)で60才以後に発病したものは135名(72%)で,病型別にみると躁うつ群,妄想群は約半数が60才以前に発病し,痴呆群,錯乱譫妄群は大部分が60才以後に発病していた.
    3) 痴呆程度は躁うつ群,妄想群,神経症群では軽度にしか認められなかつた.
    4) 遺伝負因は不明者が多く確実なことは分らなかつたが,躁うつ群,妄想群に痴呆群よりよく認められる様である.
    5) 発病に対する直接的誘因は錯乱譫妄群で全例,躁うつ群,神経症群では約半数に認められたが,妄想群,痴呆群,脳動脈硬化症群では僅かしか認められなかつた.
    6) 入院1年後の転帰は痴呆群では死亡,未治が全てで,軽快したものは1名もなく,躁うつ群,神経症群では軽快が多く未治がそれに次ぎ,妄想群では未治が一番多く次に軽快が多く,経過が長びく傾向にあり,錯乱譫妄群では死亡に次いで寛解が多く未治は全然なく患者は死亡もしくは寛解し,脳動脈硬化症群では軽決,未治,死亡が大体同じ割合に認められた.
    7) 錯乱譫妄群14名中8名は症候性精神病で,6名は心因により精神異常を示したもので症例を挙げ検討した.
    8) 以上の各群について文献を挙げて考察を行い,症候性精神病を中心として鑑別診断についても述べた.
  • 平松 直, 横山 茂生, 池田 久男, 山本 昌知
    1964 年 76 巻 1-3 号 p. 41-46
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    We reported a case of epilepsia partialis continua. Movements consist of almost continuous rhythmic jerking which is limited to the right lower limb. Angioma arteriovenosum was seen in the left parietal region on the angiogram of left carotid artery. The clonic jerk of this case was much improved on the anticonvulsants.
  • 第1編 人体H-reflexにおける促進及び回復曲線に関する実験的研究ならびに理論的考察
    神村 政行
    1964 年 76 巻 1-3 号 p. 47-62
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    Serial records of H-reflex of splnal motoneuron were obtained by the recording of action potential of soleus muscle, triggered by conditioning and testing electrical shocks on the posterior tibial nerve. The fascilitation and recovery process of the spinal motoneuron was studied by the serial records of the H-reflex. The clinical material was 30 cases including normal subjects, patients with pyramidal lesion, and patients in acute stage of cerebrel intervantion.
    In commonly, the H-reflex triggered by test shock (H) was not observed by the test shock which was given within 3 millisecond (ms') following conditioning shock, but start to appeared at 4ms appeared in peak amplitude (maximal deflection) at 6 ms., and disappeared by 9 to 13 ms. Later than 9 to 13 ms., H-reflex falled in a silent period. However, the detail of the abovementioned characteristics of the H-reflex appeared in some difference, i.e. on the patient with pyramidal lesion H-reflex continued to appear until 18ms. with maximal deflection of 150% of the H-reflex, on the normal subject those were 10 ms. and on the patient in acute stadium of cerebral surgical intervention those were 9 ms. and 50%.
    The author intended to describe the complate disappearance of H-reflex within 3 ms. following conditioning shock as the first silent period, and presumed that the first silent period would be the result of combination of refractory period of Group Ia fiber and inhibition of Group Ib fiber. The auther also would suppose the detailed characteristics of the H-reflex at the period of 4 to 10 ms. following conditioning shock was caused by the afterhyperexcitability of the motoneuron by the conditioning shock as well as summation of the test shock, but the IPSP of Group Ib efferent impulses and influence of Renshow cell were other important mechanisms. The author intended to call abovementioned brocess the fascillitatry of human H-reflex.
  • 第2編 人体運動ニウロンの動作様式に関する筋電図学的研究
    神村 政行
    1964 年 76 巻 1-3 号 p. 63-74
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    The study was performed by EMG. record of Parkinsonsism tremor and tremor-like movement of normal subject imitate to Parkinsonism tremor. H-reflex technique by electric stimulation on the posterior tibial nerve was combined.
    In grouping voltage of Parkinsonsism tremor as well as in voluntary tremor-like movement of normal subject, double discharge of the single NMU. was observed generally with a interval of 25 to 60 millisecond (ms.), and the double discharging unit was considered to be a kinetic NMU.
    H-reflex records in series by relatively long interval (low repetition) stimulation showed constant amplitude of H-reflcx. But series by relatively short interval (high repetition) stimulation showed irregular amplitude, and by shorter than 30 ms. interval stimulation H-reflex default at random. At some critical conditien e.g. 68 ms. interval at gastrocnemius muscle, 95 ms. at soleus mnscle the series of H-reflex showed two kind of amplitude, higher amplitude H-reflex and lower amplitude H-reflex appeared in each othor. Depending on the amplitude of repetitive M-reflex will not fluctuate even at short interval such as 20 ms. as already described on other literatures, the auther would conclude that above mentioned fluctuation of H-reflex amplitude was caused by the character of the afterhyperpolarization of motoneuron. The paticular phenomen of amplitude fluctuàtion of H-reflex which appcared as higher amplitnde and lower amplitude in each other would be considered due to mixing existance of two kinds neuromuscular units those have shorter and longer hyperpolarization time, and those two kinds of units would adequate to kinetic and tonic neuromusclarunits.
  • 第1編 20-Methylcholanthreneの単独経口投与による白血病の発生について
    康 逸雄
    1964 年 76 巻 1-3 号 p. 75-90
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    1) RF mice were fed three times a week wih 0.1-0.15cc of 0.4 and 0.8% 20-methylcholanthrene in olive oil by means of a needle of a catheter. The mode of development of myelogenous and lymphocytic leukemias in these mice was studied by observation of the peripheral blood picture, histological change and clinical tirsue culture. A fibrosarcoma and lungadenomas were also observed in a mouse, respectively.
    2) Leukemias induced were mainly myelogenous and their incidence was proportional to the concentrations of 20-methylcholanthrene administered. Of mice given 0.4% solutiom of the carcinogen, 17% developed myelogenous leukemia while of mice given 0.8% solution, 50% developed myelogenous and 12.5% lymphocytic leukemia.
    3) Histologically all the leukemic mice showed leukemic infiltration in many organs including the bone marrow, spleen, lymph nodes and thymus. Clinical tissue culture of these organs confirmed the diagnosis of leukemia.
  • 第2編 20-Methylcholanthrene経口投与による白血病の発生におけるcroton油のco-leukemogenic作用について
    康 逸雄
    1964 年 76 巻 1-3 号 p. 91-103
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    1) Myelogenous and lymphocytic leukemias were observed among mice given orally three times a week 0.1-0.15cc of 0.8% 20-methylcholanthrene in olive oil by means of a needle catheter and concurrently painted in the back two times a week with 5% croton oil in benzene.
    2) The incidenc of leukemias was 61.6% for myelogenous and 5.5% for lymphocytic leukemia with a total of 66.6%. On the other hand, mice receiving croton oil alone developed no leukemia.
    3) Concurrent administration of 20-methylcholanthrene and croton oil reduced the average latent period required for the development of leukemia by about four weeks in comparison with that of 20-methylcholanthrene treatment alone.
    4) From these results, it is concluded that croton oil is coleukemogenic, acting as a promoter in the 20-methylcholanthrene leukemogenesis in mice.
  • 第1編 骨髄組織培養に於ける白血球系造血機能に及ぼす影響
    岡本 迪男
    1964 年 76 巻 1-3 号 p. 105-112
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    In order to observe the influences of ATP on the bone marrow leukopoiesis, clinical tissue culture of bone marrow from normal rabbits, normal persons and patients with hypoplastic anemia was conducted. ATP solutions at various concentrations were added directly to the culture media and observations were carried out to see effect of ATP on the functions of neutrophils of bone marrow.
    As a result, it has been demonstrated that by adding an optimal amount of ATP the ontgrowth, cell density and wandering velosity of neutrophils were increased.
  • 第2編 骨髄組織培養に於ける栓球系造血機能に及ぼす影響
    岡本 迪男
    1964 年 76 巻 1-3 号 p. 113-118
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    In order to observe the influences of ATP on the bone marrow thrombopoiesis, clinical tissue culture of bone marrow from normal guinea pigs, normal persons and patients with hypoplastic anemia was conducted. ATP solutions at various concentrations were added directly to the culture media and observations were carried out to see effect of ATP on the functions of megakaryocytes of bone marrow.
    As a result, it has been seen that by adding an optimal amount of ATP the functions of megakaryocytes were accelerated.
  • 第3編 骨髄組織培養に於ける赤血球系造血機能に及ぼす影響
    岡本 迪男
    1964 年 76 巻 1-3 号 p. 119-129
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    For the purpose of observing the influences of ATP on the bone marrow erythropoiesis, a roller tube culture method was used to cultivate bone marrow cell suspensions from normal rabbits, normal persons and patients with hypoplastic anemia. ATP solutions at various concentrations were added directly to the culture media and observations were carried out.
    As a result, it has been shown that addition of ATP resulted in an increase of erythrocytes, increase of hemoglobin content and increasing percentage of orthochromic erythroblasts.
  • 第1編 肝疾患の心電図
    橋本 恭治
    1964 年 76 巻 1-3 号 p. 131-139
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    Electrocardiograms in 120 patients of liver disease (39acute hepatitis, 39 chronic hepatitis, 32 liver cirrhosis and 10 liver cancer) were studied.
    1) Pathologic findings in EKG were flatening T wave, ST depression, ST-T fusion and disturbance of conducting systems such as Ist. degree A-V block, bundle branch block and notching of R wave.
    2) Flatening T wave was found in 44 cases; ST depression in 41 cases; ST-T fusion in 41 cases. They were found more in liver cirrhosis and cancer than in hepatitis.
    3) QT interval was slightly prolonged in liver cirrhosis and cancer.
    4) QRS interval was prolonged as the disease progressed.
    5) magnitude of T wave was correlated to the serum bilirubin, colloidar reaction, BSP and gamma globulin, not to the total protein and A/G.
    6) ST-T fusion was correlated to the gamma globulin and the periods of the diseese.
    7) No correlation was found between the magnitude of T wave, ST-T fusion and serum electrolites. T/R was, however, slightly correlated to the serum potassium.
    8) Magnitude of T wave was increased as the disease became better clinically.
  • 第1編 各種薬剤の鉤仔虫障害作用
    山本 矩朗
    1964 年 76 巻 1-3 号 p. 141-149
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    The lung symptoms of Wakana disease are considered largely caused by the irritation of hookworm larvae migrated into the lung. With the purpose of killing the hookworm larvae, an anchylostomocidal experiment was conducted in vitro with chloroquine diphosphate, Stibnal, Mapharsen, Atebrin and emetin hydrochloride, and the following results were obtained.
    1. At the in vitro concentrations comparable to those attained for adults, chloroquine diphosphate showed a most pronounced anchylostomocidal effect upon Anchylostoma caninum larvae followed by Stibnal, Mapharsen, Atebrin and emetin hydrochloride in that order.
    2. The first three drugs found to be more effective against hookworm larvae in the above experiment were tested for the anchylostomocidal effect on Dubini larvae by adding the serum obtained from normal persons given intravenous injection of the adult dosage of these drugs. In this experiment, too, chloroquine diphosphate was most effective followed by Stibnal and Mapharsen.
    3. From these results, it appears that chloroquine diphosphate, Stibnal and Mapharsen act quite effectively as auchylostomocides against hookworm larvae, and good therapeutic effects of these drugs can also be expected when used in Wakana disease. Of these three drugs, chloroquine diphosphate was most effective in both experiments and together with a possible high concentration of intravenously injected chloroquine diphosphate in the lung, the agent is considered to be a drug of choice in the treatment of Wakana disease.
  • 第2編 若菜病の新治療法
    山本 矩朗
    1964 年 76 巻 1-3 号 p. 151-159
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    In view of the anchylostomocidal effect against hookworm larvae as reported in the Part I of the present studies, chloroquine diphosphate, Stibnal and Mapharsen were used to treat the lung symptoms of Wakana disease, and the results obtained are as follows.
    1. Chloroquine diphosphate administered intravenously proved to be effective in all the cases treated. There were no side effects and its prolonged administration was possible. Chloroquine diphosphate was far superior to the other two agents.
    2. Chloroquine diphosphate was less effective when administered orally than administered intravenously, although the former method of administration was more convenient and feasible.
    3. Although the effect of Stibnal was somewhat inferior to that of chloroquine diphosphate and its intravenous injection was sometimes followed by a transient increase of cough, the drug was nevertheless worthy of use.
    4. The effect of Mapharsen was inferior to the above two drugs and its side effects were more often encountered.
    5. From these data, a conclusion may be drawn that chloroquine diphosphate, particularly when administered intravenously, is indeed dramatic in relieving the lung symptoms of Wakana disease.
  • 第3編 若菜病の肺症状の成因
    山本 矩朗
    1964 年 76 巻 1-3 号 p. 161-167
    発行日: 1964/03/30
    公開日: 2009/03/30
    ジャーナル フリー
    1. Mice infected with Anchylostoma caninum larvae labeled with Fe59 showed an increase of both radioactivity and the number of the larvae in the lungs 12 hours after the infection.
    2. Mice orally infected with Anchylostoma caninum larvae were treated beginning 24 hours after the infection with chloroquine diphosphate, Stibnal and Mapharsen. Chloroquine diphosphate showed a marked anchylostomocidal effect against the larvae migrated into the mouse lungs. Stibnal and Mapharsen were also fairly effective.
    3. On the basis of the in vitro anchylostomocidal effect, clinical trial and animal experiment using mice, it is believed that these drugs severely damage hookworm larvae, especially those in the lungs, and the etiology of the lung symptoms of Wakana disease can be largely accounted for by the mechanical irritation of the larvae, although it is probable that they have something to do with an allergic phenomenon.
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