The antimitotic effect of cornin extracted from dog intestine, are summarized as follows. 1. Canine intestine cornin (CIC) inhibit the proliferation of HeLa cell in tissue culture. It's antimitotic effect, however, increases depend on the cornin dose per cell. 2. CIC is stable substance. Antimitotic effect of CIC does not decrease after stocking in desiccater over 3 years, and also 2 days preincubation in 37°C. DTT treated CIC show no differences with untreated CIC in growing HeLa cell. 3. Both dialysable and nondialysable fractions show antimitotic effect to HeLa cell. And nondialysable fraction is more effective. 4. Ultrafiltration is done by Diafilter membrane. The most effective fraction of molecular weight is over than 55, 000. The next is molecular weight 20, 00055, 000, the third is molecular weight below 10, 000. The component molecular weight 10, 00020, 000 is slightly effective. 5. The antimitotic effect of molecular weight over than 55, 000 fraction decreases by 2 days treatment in 0.025% trypsin at 37°C. 6. CIC is separated in 3 fractions by Sephadex G200 column. The most effective fraction is FIII. It suggests that the antimitotic action of CIC is more effective in relatively small molecular weight of molecular weight over than 55, 000 fraction.
With the purpose of elucidating the role of the stroma for the growth of tumors, the vascular structures in the parenchymal stroma and the surroundings of animal tumors were studied. In the first chapter, the vascular structures of Bashford tumors and Ehrlich subcutaneous tumors were examined employing the India ink method. The Blood vessels of tumors are derived from their surrounding host connective tissue. The structure of blood vessels of every tumor has features of its own. Further, it was observed that metastatic tumors in the lungs of Ehrlich ascites carcinoma and Brown-Pearce tumors had an individuality in their vascular structures. In the second chapter, the vascular structures of Ehrlich subcutaneous tumors and first generation transplants of C3H spontaneous mammary cancers were observed employing ultrasoft X-ray, and the relationship between the blood vessels and the stromaltissue of these tumors was investigated using several special staining methods. Characteristic features were also observed on the vascularization and the stromal tissues of Ehrlich subcutaneous tumors treated with chondroitin sulphate. In the third chapter, the influences of administration of Bleomycin on C3H spontaneous mammary cancers were abserved with respect to the vascular struture and stromal tissue. It was found that small tumor vessels were narrowed, obstructed and destroyed by administration of this drug. Further, it was observed that the vasculature and stromal tissue of lungs of mice and humans administered with this agent were likewise damaged, and that prednisolone and chloroquine considerably inhibited these pulmonary damages caused by this drug.
The author calculated, using Pasquill's chart, the logical concentration of sulfur dioxide concerning principal 15 chimneys in many places of Mizushima industrial area which had emitted 74 percent sulfur dioxide over Mizushima, and compared concentrations measured practically at the respective station of Fukuda-cho, Mizushima Harbor Office and Amagi with the logical concentration calculated by Pasquill's chart. The results obtained were as follows. 1. The high concentrations of sulfur dioxide measured practically at Fukuda-cho station could be logically explained by the diffusion called C-stability following Pasquill, and those at Amagi station could be also explained by the diffusion called D-stability. 2. Under the condition of each of A and B stabilities following Pasquill, sulfur dioxide diffused itself significantly on C-stability in Pasquill's chart, and in the case of each B and C stabilities, it diffused itself on D-stability in Pasquill's chart.