We developed a new immunological method of determinating urinary HCG (Human Chorionic Gonadotropin), in which antigen-antibody reaction is allowed to proceed while HCG is being adsorbed to kaolin particles. With this method, we conducted some immunological studies with normal and abnormal pregnancies, and obtained the results as follows. 1. The urinary HCG concentration in normal pregnancies began to increase by about 7 gestational weeks, which reached its maximum at 12 weeks, thereafter it decrease gradually. 2. In the cases of abortus imminens the urinary HCG concentration was somewhat over than that in normal pregnancy, but in those cases whose prognosis was poor, HCG concentration was markedly low. 3. In the cases of hydatidiform mole the urinary HCG concentration was found to be abnormally high in two cases out of three, but it became negative (500iu/l) by 23 to 40 postoperative days. 4. As to the correlation between Friedman's reaction and our determination method we found the correlation quotient to be 0.583, indicating a moderate correlation. 5. The threshold of Friedman's reaction was found to be within 7.5 to 10.0iu.
The sinus node of the canine heart contains numerous autonomic nerve fibers. All of these are unmyelinated fibers associated with the cytoplasm of Schwann cell. The axon terminals, however, are devoid of such processes and are separated from the myocardial cells by an unusually wide space. In these naked nerve ending, 3 types of vesicles are observed: small granular vesicles, large granular vesicles, and agranular vesicles. The distribution of the vesicles by KMnO4-fixation has enabled us to classify autonomic nerve endings into 3 categories.
The monoaminergic innervation of the mammal (ox, monkey, dog, cat, rabbit, guinea pig, rat and mouse) heart was studied by a histochemical fluorescence technique. The number and distribution of monoaminergic nerve terminal was greater in the atria than in the ventricles of the heart of mammals. Fluorescence histochemical studies of the mammal heart conducting system have shown the density of monoaminergic nerve terminals to be highest in the sino-atrial node, followed in order by atrio-ventricular node, atrio-ventricular bundle and false tendons. The plexus was so extensive that the majority of sino-atrial nodal cells appeared to be innervated. The innervation to the Purkinje cell in the false tendons from both the right and left ventricle of mammals except for ox was of low density and a large number of cells seemed to have no contact at all with the monoaminergic terminals. In the cases of 6hrs after reserpine administration, fluorescent nerve fibers had disappeared from all mammal heart. In cases in which L-DOPA oder nialamide was administered 3-5hrs before killing, fluorescent nerve fibers in mammal heart gave an intensified fluroescence together with the increased number of fluorescent fine varicose nerve fibers. After right and left stellate ganglionectomy, histochemical determinations showed a decrease in monoaminergic nerve content both right and left atria, in both right and left ventricle, and heart conduction system.
A study on coagulation-fibrinolysis system in surgical vascular disease was made to search for genesis of thrombosis and its therapy. Special consideration was taken into the availability of fibrinolytic enzyme for thrombosis. The results obtained in the study were as follows: 1) A marked increase of fibrinolytic activity was observed in most cases of aneurysm of the aorta, especially dissecting aneurysm, and antiplasmin level was low. 2) In arterial embolism, plasminogen activator was released from the site of a new thrombus into the blood. Then the activator spread throughout the entire body. The fibrinolytic activity reached the maximum level about 6 hours later and was reduced in a short time. 3) In phlebothrombosis, especially in the occlusion of the inferior vena cave, antiplasmin level was reduced. 4) Inmost cases of hemangioma, a marked increase of fibrinolytic activity was found in the blood drained from the affected area. Even though there would be no change of fibrinolytic activity in the systemic blood, laboratory data obtained from the local blood often showed those compatible with intravascular coagulation syndrome. 5) Postoperative use of hemostatics in vascular surgery had to be cautious, because of increased coagulation activity and decreased fibrinolysis activity that were induced by operation. Rather anticoagulation therapy might be necessary to prevent re-obstruction of the reconstructed vessels in occlusive vascular diseases. 6) Therefore, Urokinase would be better as a fibrinolytic agent in term of less side-effect and low antigenecity. 7) As arterial thrombus in the extremities was hardly dissolved with fibrinolytic agents and caused severer symptoms, reconstruction of an occluded artery should be performed as soon as possible and be followed by anticoagulant therapy. 8) Venous thrombus would be dissolved with fibrinolytic enzymes, if they were given within 3-4 days after the onset of symptoms. Hence it would be important to start with fibrinolytic therapy as soon as possible, rather than with surgical invention. Even if thrombus was not dissolved completely, collateral circulation would be improved enough to rehabilitate a patient without complaints. 9) Dosage of thrombolytic agents was determined from the following formula: Units of Urokinase to dissolve a clot at 6 hours × Estimated circulating blood volume=A daily dosage of Urokinase. Urokinase was administerd intravenously by continuous drip infusion with heparin and low molecular weight dextran. During the administration euglobulin lysis time was maintained at less than 60 minutes.
Osteopetrosis (Albers-Schönberg's disease, marble bones) is a rare disease characterized by generalized increased density of the skeleton. A case showing typical roentgenological findings of the disease is presented and following a brief review of the literature, the roentgenographic characteristics are discussed.
For the purpose of clarifying the water pollution of Lake Kojima, two samples of water were collected and analyzed from each surface and bottom at 7 different parts in the lake. The results are summarized as follows. 1. The lake has been remarkably tainted with organic substances as grasped from the result of COD. 2. There were nutritious salts in high concentration, consequently accelerating a eutrophication. Especially, the bottom water contained a large quantity of NH4+ because of anaerobic decomposition. 3. In summer, an overgrowth of phytoplankton was observed and pH of surface water shifted to respectable alkaline in consequence. 4. The conversion of Lake Kojima to fresh-water had not been succeeded. For the preventive measure of eutrophication, the exhaust of nitrogen and phosphorus compounds should be controled. If not so, the condition of Lake Kojima will turn to inappropriate to fish subsistence.
The authers reported the results of field survey on acatalasemia and hypocatalasemia during the years of 1970 and 1971. Six hypocatalasemias were seen in the tests of 3, 593 individuals in several parts of Japan. No acatalasemic individuals were reported since 1968. Consequently, the total of reported acatalasemias were 96 cases of 46 families by the end of 1971.