The antimitotic effect of cornin extracted from bovine liver was studied. Obtained results are summerized as follows.
1) Bovine liver cornin has antimitotic effect on sea urchin eggs. But the author has not recognized the morphological differencies on their development when observing sea urchin eggs under the electron microscopy.
2) The mitotic rate of Ehrlich ascites carcinoma cells decreased by injections of bovine liver cornin and also the DNA synthesis was inhibited in propotion to the doses of bovine liver cornin.
3) The mitotic rate in regenerating rat liver decreased by injections of bovine liver cornin.
4) The inhibition of bovine cornin on the DNA synthesis in regenerating rat liver, was the most strongest when administered in equal to the physiological concentration.
5) Above results suggest the existense of the higher mitotic autoregulating center.

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A comparative study was made on the uptake of intra-peritoneally administered radioiron by catalase of blood, liver, and, bone marrow in guinea pigs and rabbits. Radioactivity of ⁵⁹Fe per catalase activity (c. p. m./K cat/mg protein), viz., specific activity, was greatest in marrow, followed by liver and blood. Maximum incorporation of ⁵⁹Fe in liver catalase was attained on the 6th day, and in marrow catalase on the 9th day. In blood catalase, however, specific activity continued to increase gradually through the entire experimental period.
These data showed that blood catalase is different from liver catalase in its origin and originates, probably, in bone marrow, And it was demonstrated by these rapid turnover of ⁵⁹Fe that in liver and bone marrow catalase was, labile.
Catalase solutions, then, were evaluated by means of starch block electrophoresis. In each catalase, which is recollected from starch block after electrophoresis, ⁵⁹Fe incorporation curve was similar to the results above described, so it was made clear that origin of blood catalase is different from that of liver catalase.
Last, some studies were made on the inhibition of catalase activity by X-radiation on mice to cofirm, mainly, that blood catalase originates in bone marrow. On the rate of decrease of catalase activity after X-radiation, there were obvious difference between blood and liver. Activity of liver catalase decreased about 20% at the beginning and about 40% at the end of this experiment. On the contrary, the rate of decrease of catalase activity of blood was slight only about 5%. On the last day of the experiment (the 12th), not only catalase activity of blood but hemoglobin cancentration showed sudden depression which indicates the destruction of bone marrow by X-radiation.
From these facts, it proved that the origine of blood catalase and liver one are different, and that blood catalase originates in bone marrow.

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To establish the diagnostic criteria on X-ray findings for the stomach mass examination survey film, comparative study on the X-ray findings of the mass examination survey films with the findings of the resected stomach had been done. For this purpose, I have studied 74 lesions in 47 cases. The results were as follows:
1) About 80% of them were demonstrated as the filling defect. The relations among the demonstration of the filling defect and the size of the lesion, and pathohistologic findings were as follows:
I) The lesions measuring over 4 cm in diameter were demonstrated as the irregular filling defect.
II) The lesions with the size of 2 to 4 cm in diameter were demonstrated halves as the irregular filling defect and halves as a faint filling defect.
III) The lesions measuring within 2 cm in diameter were demonstrated as the faint filling defect.
IV) As to the pathohistologic findings, many of the advanced cancer were demonstrated as the irregular filling defect but early stomach cancer and the benign lesions had no such plain findings.
2) The small lesions not demonstrated as the filling defect were demonstrated as a irregular margin, abnormal relief view. etc. These X-ray diagnosis were needed in order to detect the small protruding lesions.
3) The Barium filled stomach in prone position was most important and upright position was also important.

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With the same intention and in the same method as that in part 1, I studied 81 concaving lesions in 71 cases which were detected by mass examination survey and were operated. The results were as follows:
1) As to the concaving type, irregularity and rigidity of the stomach wall, shortened lesser curvature and abnormal relief view were the important findings to detect the lesion.
2) 2. 3 findings were found in every lesion.
3) A finding was detected in 1. 9 sheet of films out of 5. The irregularity of the wall, rigidity of the wall, unusual indentation of stomach wall, irregular filling defect and deformity of the antrum were detected in more than 2 sheet of films but widened stomach angle and abnormal relief view were detected only a sheet of film.
4) The barium filled stomach in upright position was important and effective to detect the lesion. On the contrary, prone and upright left oblique position was uneffective. Many of the irregularity and rigidity of the stomach wall, widened stomach angle and shortened lesser curvature were detected by upright position. Abnormal relief view was detected by supine position.
5) A few lesions within 2 cm in diameter could not be detected. The bigger the lesions were, the more frequently the rigidity of the stomach wall and the abnormal relief were found.
6) In the lesion around the angle, widened stomach angle and filling defect were frequently found. In the lesions around the corpus, nische and fleck were more frequently found.

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Localized stereotaxic lesions were placed in the pulvinar nuclear group of the thalamus, viz., the medial, lateral and inferior pulvinar nuclei and the suprageniculate nucleus, and the ensuing fiber degeneration was traced to the cerebral cortex by the method of Nauta and Gygax (1954).
Following lesions of the medial pulvinar nucleus, cortical degeneration is found diffusely in the middle and posterior portions of the lower bank and fundus of the middle suprasylvian sulcus and the adjacent part of the posterior ectosylvian gyrus as well as in the posterior bank of the posterior suprasylvian sulcus. However, the severely degenerated portion in these cortical areas varies with the site of the lesion in the medial pulvinar nucleus. In the heavy cortical projection of this nucleus, there appears to be a reversal in the anteroposterior dimension; the rostral part of the nucleus projecting to the posterior portion of the inferior bank of the middle suprasylvian sulcus and the adjacent part of the posterior ectosylvian gyrus, and the caudal part projecting more rostrally in these cortical areas. In cases with lesions in the ventral part of the medial pulvinar nucleus, light degeneration is found in the anterior sylvian gyrus.
The lesions in the lateral pulvinar nucleus produce cortical degeneration in the middle and posterior suprasylvian gyri with its lateral sulcal wall and in the zone of confluence of the posterolateral and posterior suprasylvian gyri. Severe degeneration is, however, confined to the middle suprasylvian gyrus and the zone of confluence of the posterolateral and posterior suprasylvian gyri. Scarcely any degeneration is found in area 19 in the lateral cortex in most of our cases with lesions in the lateral pulvinar nucleus, but scattered degeneration is seen in the medial cortex around the splenial sulcus, including area 19. There appears to be an anteroposterior organization in the heavy cortical projection of the lateral pulvinar nucleus.
After lesions in the inferior pulvinar nucleus, degeneration is seen in the medial (area 19) and lateral banks of the lateral sulcus and the upper bank of the middle suprasylvian sulcus. In addition, light degeneration is found in parts of area 18 and the crown of the suprasylvian gyrus.
The lesions of the suprageniculate nucleus lead degeneration to the cortex around the anterior half of the middle suprasylvian sulcus and in the anterior sylvian gyrus and the adjacent posterior bank of the anterior ectosylvian sulcus. The suprageniculate nucleus appears to project to these cortical regions in a topical manner in the anteroposterior dimension.

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The precise mensuration of the intracellular cation content is very significant from the biological point of view. When a salt concentration is medium is higher than in bacterial cells, it is very difficult to measure the intracellular cation content because of extracellular high contamination. In such a case, there is no other way except repeated washing for elimination of the outer contaminants. With the repeated washing method, authors attempted to measure accurately the intracellular cation content of Staph. aureus 209 P, cultivated in the medium of the various degrees of salt concentration, by flame photometory and atomic absorption spectrophotometory.
The results were as follows.
1. When univalent cation concentration in cells is lower than in medium, it is necessary to wash the cells several times. The washing effect on extracellular contaminants could agree with the theoretical equation of washing that the contaminant decreased exponentially. The successive decrease of assay values by an easy grade must show a leakage of the intracellular Na. The reflective point could be considerated to show the Na content.
2. As in general potassium concentration in cells is higher than in medium, the accurate value was decided by extrapolation of the linear analytical quantity vs. washing time to zero time.
3. In spite of the extreme variation of sodium salt concentration in culture medium, the univalent cation content in Staph. aureus 209 P had hardly any shifts.
4. Bivalent cations, Mg and Ca, were not affected by the washing.

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In these experiments, two kinds of extracts, Mytilus cornin-nondialyzable fraction and Mytilus Li-ethanol fraction, were used to study the inhibitory effects on Ehrlich ascites carcinoma. And also investigated the comlexing action of extracts with ZnCl₂, MnCl₂, CuCl₂ and adrenaline. In studying carcinostatic action the author used ddN strain mice. These were inoculated routinely with 1×10⁷ Ehrlich ascites tumour cells in a volume of 0.2ml of ascites fluid. Then 48 hours after inoculation, treatment with the extracts was begun. Each day for five days, each one of the mice was given intraperitoneally injection with a solution containing 5-20mg of the material per 0.5ml of saline. Obtained results are summarrized as follows.
1) Mytilus cornin and Mytilus cornin-nondialyzable fraction had no effect on ascites bearing mice.
2) 5-20mg of Mydilus cornin-nondialyzable fraction in 10^-510-6M of ZnCl₂-saline injected intraperitoneally into ascites bearing mice for five days produced a marked reduction in mean body weight and an increse in survival time.
3) In the cace of MnCl₂ and CuCl₂ a similar effect was demonstrated in the reduction of body weight, but not in sarvival time.
4) Mitilus Li-ethanol fraction in saline led to inhibition of Ehrlich carcinoma, and also an increase in survival time, when it was given intraperitoneally at dose level of 10mg per mouse on the seconed day after tumour transplantation and continued for each of five days.
5) Mylilus Li-ethanol fraction in heavy metal ions and adrenaline had no effect on ascites bearing mice.

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Followings are the results derived from the treatment of malignant tumors of animals with the fibroblast-inhibiting agent such as phytonadione based on the unique idea of ours.
1) No effect was observed on the catalase activity of liver tussue homogenate of normal healthy mice by the direct addition of excessive doses of phytonabione.
2) No change was observed in the liver catalase activity of normal mice abministrated 50mg/kg of phytonadione.
3) Inhibition of decreasing liver catalase activity was observed at two weeks after abministration of phytonadione in mice bearing sarcoma-180 ascites and subcutaneous tumors.
4) No inhibition of decreasing liver catalase activity was observed at one week. after administration of 50mg/kg of phytonadione in mice bearing Ehrlich subcutaneous cancer.
5) With the combination use of phytonadione and one of the fibroblastinhibiting agents, chloroquine, a more remarkable inhibiting effect of decreasing liver catalase activity was observed than with a single abministration of chloroquine in mice bearing asrcoma-180 ascites tumor.
6) Remarkable carcinostatic effect was observed with the combination use of mitomycin-C and phytonadione than with the single administration of mitomycin-C.

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Followings are the results derived from the treatment of mesenchymal malignant neoplasms with a fibroblast-inhibiting agent such as chloroquine, based on the unique idea of ours.
1) One of 3 cases of lymphosarcoma showed a complete remission.
2) All the 3 cases of giant follicular lymphoma showed a diminution of lymph nodes, and they were thought to be almost in a complete remission.
3) One case of fibrosarcoma showed a diminution and necrosis of tumors.
4) No effect of chloroquine was observed in such case as reticulum cell sarcoma, Hodgkin's disease and synovial sarcoma.
5) Lymph node diminution was observed in acute and chronic lynphocytic leukemias, but they did not reach to a complete remission.
6) One of 4 cases of chronic myelogenous leukemia showed a reduction of hepatosplenomegaly, a decrease of white blood cell counts associated with hematological improvement, but it did reach to a complete remission.
7) Side effects of chloroquine were observed in 6 of 24 cases, and included slight gastrointestinal symptoms. However, they were alleviated by either discontinuance or dose decrease of chloroquine. Since severe side effects such as retinopathy have been reported, periodical ophthalmological examination is necessary.

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