SD was seen in 40 (7.8%) out of the 511 Patients who had been treated with glucocorticoid hormon (GCH); most frequently in the patients with leukemia (13 cases) followed by aplastic anemia (12 cases). Incidence of SD was 29.3% in aplastic anemia, 25.0% in hemolytic jaundice, 22.2% in other blood dyscrasias, 11.8% in bronchial asthma and 11.3% in leukemia.
The following factors were apparently related to elicit high incidence of SD; the age ranged from 51 to 60 years old, an averaged total dose given corticosteroids was 1666.7mg, an averaged period during which GCH has been given was 83.5 days and an averaged total amout of blood transfusion was 4138.8mg
In the cases manifestign SD, 50g glucose tolerance test (GTT) has shown always diabetic type and (immuno-reactive insulin (IRI) has displayed usually hyperresponse and delayed type of response curves. Concerning the serum lipids, level of the cholesterol was often elevated and an increase of palmitic and a decrease of linoleic acid were revealed in percentage composition of total fatty acids.

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1) The effect of glucocorticoid on 1-¹⁴C-acetate incorporation into major lipids classes and each fatty acid.
Male ICR mice were injected intramuscularly Dexamethasone 1.33mg/kg for five days in one group, for ten days in the other and not injected as a control. One hour after injecting ten microcuries of 1-¹⁴C-Acetate intraperitoneally, ¹⁴C was recovered in the whole blood and liver of the both groups, and activities were increased in whole blood and decreased in liver of the ten-day group.
¹⁴C was recovered mainly in the triglyceride and phospholipid among major lipid classes of peripheral blood and liver, and there was a negative correlation of radioactivities of triglyceride and phospholipid between the liver and whole blood during the treatment with steroid. Radioactivity recovered in the oleic acid was decreased in percentages among total fatty acids and esterified fatty acids of phospholipid and of triglyceride.
Therefore, it is considered that mono-unsaturation mechanism in fatty acid might be supppresed by steroid treatment, whereas a relative increase of recovery of ¹⁴C was observed in fatty acids with longer chain than linoleic-acids chain.
2) Effect of GCH on conversion of 1-¹⁴C-linoleate and 1-¹⁴C-linolenate in the total fatty acids of the liver to other several fatty acids with long chain.
Ten microcurie each of 1-¹⁴C-linoleate and 1-¹⁴C-linolenate were mixed with human serum, albumin, agitated at 55°C for 30 minutes and injected intraperitoneally into the three groups mentioned above. One hour later the animals were killed and their livers were excised for assessment. A few conversions of 1-¹⁴C-linoleate into longer and shorter chains of fatty acids were observed in the both groups treated with steroid, but 1-¹⁴C-linolenate was converted significantly and highly to other longer chain of fatty acids, especially in the five-day group.
3) The effect of anabolic steroid hormon on lipids metabolism in liver and whole blood.
Oxandrolone 4mg/kg body weight was given orally to the two groups treated with steroid, and non-treated group with oxandrolone and steroid was kept as control. Loss of body weight and elevation of serum lipids appeared to be less than in the group treated with only GCH. Concerning the study on main lipid fractions of liver, when Dexamethasone was injected together with Oxandrolone, percentage of incorporation of 1-¹⁴C-acetate into triglyceride was reduced, whereas that into phospholipids was increased.

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Serum α₁_acid glycoprotein (α₁_AG) content, lactic dehydrogenase (LDH) activity, cholinesterase (Ch-Ease) activity, plasma fibrinogen (Fbg) content, hemoglobin content, serum albumin content and erythrocyte sedimentation rate were measured in 117 patients with lung cancer. Significant difference was observed in α₁_AG as well as Fbg between stage I and II, in Ch-Ease between stage II and III and in LDH between stage III and IV. In inoperable cases who did not obtain any clinical improvement during chemotherapy, significant positive correlation was observed between their life spans and α₁_AG as well as Fbg measured prior to chemtherapy, and as for Ch-Ease negative correlation was observed. More close correlation was obtained between life spans and biological status which was set up following their laboratory data as stated above. Therefore, it seems that biological status are reliable index for estimating life spans of inoperable cases.

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The first part of this paper is concerned with the distribution of organo-chlorine pesticides in mice using a liquid scintillation counter, and an attempt was made to make clear its mode of action.
¹⁴C-DDT and ¹⁴C-Dieldrin were given orally to mice.
It was found that after 24 hours, both compounds were taken up by all tissues examined (liver, kidneys, heart, lungs, spleen, brain, with high concentrations in fat tissues).
Their concentrations gradually increasd, reaching the peak levels after 48 hours and therafter gradually decreased, but even after 30 day a small amount of these compounds was still, detectable in most organs, especially in fat tissues.
Since these compounds once deposited in tissues, remain unexcreted for a long period of time, much attention should be paid to prevent their chronic intoxication.

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The second part of this paper concerns whole body autoradiography and results obtained similar to those reported in the first paper.
Both ¹⁴C-DDT and ¹⁴C-Dieldrin were taken up by all tissues 12 hours after their oral administration with high concentrations in fat tissues, next in liver and kidneys.
Deposition in fat tissues increased gradually and then decreased gradually, but even after 5 days, these compounds were found in all tissues, especially more in fat tissues.
Another interesting point is that these chemicals were also taken up by the bone marrow.
Therefore, hypoplastic anemia or other blood dyscrasias may have something to do with this observation.
These results suggest that once being taken up, organo-chlorine pesticides remain in animal tissues for a prolonged period of time, and the repeated, excessive exposure to them may, prodce chronic poisoning or various deleterious effects on human bodies.

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With cancer-bearing mice as the subjects of our study, we studied allogeneic inhibitory activity of lymphocytes which constitutes the center of the immunological surveillance system believed to be protecting mammalians from cancer, and obtained the following results.
At first, methylcholanthrene-induced sarcoma (MC-sarcoma) was isografted to C₃H mice subcutaneously on the back between scapulae.
Then the regional axillary lymph nodes were taken out and lymph node cells were isolated from these nodes. These lymph node cells were mixed with JTC-11 cells derived from Ehrlich cancer cells in the ratio of 40:1, and the mixed cells were cultured for 24 or 48 hours. During this culture the increase in the number proliferating JTC-11 cells, counted every week, was taken as the allogeneic inhibitory activity of the regional lymph node cells. As a result it has been found that in the early stage of one to two weeks after tumor transplantation there can be observed an allogeneic inhibitory activity as strong as that of normal mouse lymph node cells, but by the third or fourth week such an allogeneic inhibitory activity of the regional axillary lymph node cells has been completely lost. In other words, it seems that in the case of progressive cancer the allogeneic inhibitory activity decreases along with time.

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Visual evoked responses (VER) were recorded from the thalamus and the scalp during stereotaxic thalamotomy in our service. The study involved fourteen patients with various involuntary movement disorders and intractable pain. Routine monopolar EEGs were recorded, from the ventrolateral nuclens and the centremedian of the thalamus, with multipolar electrodes and from the vertex and the occipital region with needle electrodes simultaneously. VERs have been analysed by means of averaging techniques as well as spectral analysis of the EEGs.
Experimental studies were also performed with chronically implanted electrodes in the ventrolateral nucleus, the lateral geniculate body, the visual cortex and the anterior sigmoid gyrus of the cats. The results were as follows:
1) Visual evoked responses (VERs) from the occipital regions were well demonstrated in seven out of fourteen cases. The responses had typical seven components (I-VII) with rhythmic after-discharge, documented by Cigánek's report.
2) The evoked responses from the vertex showed practically no early components. Therefore, four components of the response, namely P₁, N₁, P₂ and N₂, were started with P₁ of the peak latency of 105.2msec. This response was corresponded to so-called vertex potential, of which pattern had no similarity to the VER from the occipital region.
3) The evoked responses from the human thalamic VL and CM nuclei showed very similar patterns to the vertex potentials with four components. Peak latencies of them were noted P₁ with 96.4±12.8msec, N₁ with 134.6±19.4msec, P₂ with 196.0±25.5msec and N₂ with 260.9±29.3msec. The thalamic responses always tended to lead off the vertical one about 8.8 to 18.3msec in all cases.
4) In the animal (cat) experiments, VER from visual cortex showed similar pattern to the one from the lateral geniculate body, and VER from the thalamic VL nucleus resembled to the one from the anterior sigmoid gyrus.
5) Rhythmic after-discharges were noted in the thalamus as well as the vertex after their wave components. The after-discharges of the vertex were not disappeared after VL thalamotomy, while spontaneous spindle discharges were disappeared after the thalamotomy. Therefore, rhythmic after-discharges were thought to be involved some other physiological mechanisms to that of spantaneous spindle activity.
6) Spectral analysis of EEGs showed high value of coherence between the thalamic and thevertical EEGs before and during photic stimulation. On the other hand, low coherence were obtained between the vertical and the occipital EEGs in all cases.
7) Photic stimulation provoked VERs in the occipital cortex as well as the vertex potentials through the VL or CM nucleus of the thalamus. It is most interesting that, while the VL nucleus is known as one of specific thalamic nuclei physiologically, the nucleus is also concerned with one of non-specific response such as vertex potentials.

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Fibrinolysis of bone marrow aspirates and peripheral blood from patients with various blood diseases was studied by thrombelastography. The fibrinolytic rate of bone marrow aspirates was compared with that of peripheral blood, and it was disclosed that the fibrinolytic activity of the former was always higher than that of the latter at 1 and 2 hours of observation. Alteration of the fibrinolytic activity of bone marrow aspirates preceded that of peripheral blood in accordance with the clinical conditions of diseases. This tendency was pronounced in some cases of acute leukemia. It was also demonstrated that addition of antiplasmin agent such as ε-ACA and trans-AMCHA clearly suppressed the fibrinolytic activity.
A few cases of ITP, pure red cell aplasia, ALL and Banti's syndrome were found to exhibit a new pattern of thrombelastogram.

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The englobulin fraction was extracted from bone marrow aspirates and peripheral blood of patient with various blood diseases. The fibrinolytic activity of this fraction was measured by the standard and heated fibrin plate method. The fibrinolytie activity of bone marrow aspirates was found to be always higher than that oi peripheral blood in normal and pathological conditions. The level of plasminogen activator of bone marrow aspirates was also higher than that of peripheral blood, particularly in leukopoietic and thrombopoietic disorders as well as in dysproteinemias. This finding suggested that plasminogen might be activated to plasmin in bone marrow.

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