岡山医学会雑誌
Online ISSN : 1882-4528
Print ISSN : 0030-1558
94 巻 , 3-4 号
選択された号の論文の20件中1~20を表示しています
  • 和田 豊治
    1982 年 94 巻 3-4 号 p. 165-177
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    The significance of immunity in lung cancer patients has remained unclear. In this report, the relationship between the leukocyte adherence inhibition test in glass test tubes (tube LAI test) which seemed to be a tumorspecific immune response and the delayed hypersensitivity (DH) skin test was studied.
    The tissue extracts from squamous cell carcinoma, adenocarcinoma of the lung and adenocarcinoma of the colon were prepared for the tube LAI test on 40 lung cancer patients. Thirty-two patients out of 40 were also examined by a battery of DH skin tests including PPD, Candida, SK-SD and PHA.
    The follwoing results were obtained.
    1. Lung cancer patients showed a significantly high nonadherence index (NAI), calculated from the difference of the numbers of non-adherent cells in the presence of tissue extracts, and 35 per cent of them were positive for the tube LAI test.
    2. The positive cases with the tube LAI test seemed to react more specifically with extracts from the same histological type.
    3. Eight cases out of 13 (61.5%) in stage I and II, and 6 cases out of 27 (22.2%) in stage III and IV were positive for the tube LAI test. Early stage cases had a high incidence of positive tube LAI test.
    4. Eleven cases out of 13 (85%) with positive tube LAI tests showed normal DH skin reactions. On the other hand, 11 cases out of 16 (69%) with positive DH skin tests were related to positive tube LAI tests.
  • 西山 正一
    1982 年 94 巻 3-4 号 p. 179-188
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    I reported previously that cell malignancy was promoted significantly by treatment with 3'-Me-DAB in the presence and absence of S15 mixture. In this report, I examined the effect of treatment frequency of 3'-Me-DAB in the presence and absence of S15 mixture on a cloned rat liver cell line (CL-20) which had low-tumor producing capacity. Tumors produced by subcutaneous back-transplantation of CL-20 cells treated with 3'-Me-DAB in the presence and absence of S15 mixture showed more undifferentiated, solid and infiltrative morphology than that of non-treated control cells. The tumors produced by back-transplantation of CL-20 cells treated with high concentrations of 3'-Me-DAB showed metastatic growth in the lung of rats, but those produced by back-transplan-tation of untreated CL-20 cells did not. Plating efficiency increased in proportion to the treatment frequency of 3'-Me-DAB in the presence and absence of S15 mixture. This fact suggests that plating efficiency is effective as a marker for the promotion of 3'-Me-DAB-induced cell malignancy. The aggregate sizes formed by the rotation culture were larger in the cells treated with 3'-Me-DAB in the presence and absence of S15 mixture than the control cells. However, the effect of the treatment frequency of 3'-Me-DAB was rarely found in this experiment. Few changes were observed with other in vitro markers for the promotion of cell malignancy. These findings suggested that cell malignancy could be promoted by repeated treatments of 3'-Me-DAB in the presence or absence of S15 mixture in vitro.
  • 鈴木 芳英
    1982 年 94 巻 3-4 号 p. 189-200
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    Intra-operative left ventricular functions were measured by epicardial echocardiography, which was obtained by small ultrasound transducer (5 megaheltz) on the epicardium of the right ventricular outflow.
    Canine left cardiac functions after cardiopulmonary bypass were compared in 2 groups, one being 60-minute cardiac arrest with cardioplegia (group A) and the other 45-minute ischemic arrest (group B).
    The results obtained were as follows:
    1. It was simple and reliable to determine the state of left ventricular function with epicardial echocardiography during operation.
    2. There was good correlation between the values for cardiac output as measured by thermodilution and calculated by epicardial echocardiography.
    3. Contractility after cardiopulmonary bypass was well maintained in both groups. Some indices of systolic cardiac function in group B after cardiopulmonary bypass were compromised, but those of group A remained normal.
    4. The posterior left ventricular wall motion in diastole in group B was slower than that in group A after cardiopulmonary bypass. Ischemic arrest (group B) was shown particularly to impair myocardial relaxation and to raise left ventricular wall stress in diastole.
    It was concluded in the present study that impairment in cardiac function appeared first in diastole following cardiac surgery and that epicardial echocardiography would be a convenient and simple tool for determining cardiac function during surgical intervention. It was also found that cardioplegia preserved cardiac function near normal level even in diastole.
  • 藤原 徹
    1982 年 94 巻 3-4 号 p. 201-210
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    In human renal allografts, recipients respond with various specific immune reactions, such as the production of immune complexes. The circulating immune complexes provoke damage of the grafted kidney under the appropriate condition. Therefore, the detection of circulating immune complexes is important in predicting the rejection of grafted kidneys. Several methods of measuring immune complexes have been reported. In this study, sera from 25 patients who had received renal allografts were studied for the presence of circulating immune complexes by using a EAC rosette inhibition test.
    Sera from 25 patients were divided into three groups (1. acute rejection, 2. chronic rejection, 3. no rejection episode) and EAC rosette inhibition tests performed. Serum samples from the chronic rejection group gave high inhibition of EAC rosette formation, showing good correlation with clinical chronic rejection episodes.
    It was concluded that circulating immune complexes play an important role in chronic rejection. Therefore, the EAC rosette inhibition test is useful for predicting chronic rejection, and measuring circulating immune complexes.
  • 廣田 晴郎
    1982 年 94 巻 3-4 号 p. 211-220
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    Dogs were exposed to either trichloroethylene (TCL) or 1, 1, 1-trichloroethane (TCE) in order to investigate the effects of these solvents on hematologic parameters. Furthermore, because of unusual changes in white blood cell counts following exposure of pregnant dogs to these solvents, investigation of the effects of several hormones given prior to exposure to the solvents was pursued.
    The results obtained were as follows.
    1. White blood cell counts showed a marked decrease immediately after starting exposure to TCL or 1, 1, 1-TCE, though the decrease was temporary. On the other hand red blood cell counts, hematocrit values and blood platelet counts showed no changes.
    2. While each kind of white blood cell decreased in number, the degree was greatest in neutrophils.
    3. TCL caused a greater decrease in white blood cell counts than 1, 1, 1-TCE.
    4. A dose-response relationship was seen between the concentrations of 1, 1, 1-TCE from 200 to 700 ppm and the decrease in white blood cell counts.
    5. No change in white blood cell counts was observed following the exposure of pregnant dogs to 1, 1, 1-TCE.
    6. Premedication with estradiol, progesterone and hydrocortisone had a preventive effect on the decrease in white blood cell counts caused by the exposure to 1, 1, 1-TCE, but no such effect was seen with premedication of testosterone.
  • 玉井 豊理
    1982 年 94 巻 3-4 号 p. 221-234
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    Part 1
    A method of evaluating the effective liver volume has been devised for liver-scintigraphy. Matrix ROI (region of interest) was set on the anterior liver scintigram with Au-198 colloid and the depths under matrix ROI were calculated by the use of the plateau values of the histograms and the absorption coefficient (formula 2). The effective liver volume was the sum total of the areas of matrix ROI to the depths. The effective liver volume was calculated as follows:
    I=∫woIoe-μxdx…………(1)
    Io: γ-ray intensity of unit depth
    Ip: γ-ray intensity of w depth
    w: depth of radioactive substance
    μ: absoption coifficient
    therefore
    w=-1/μ1n{1-Ip/Io(1-e-μwo)}……………(2)
    Ipo: γ-ray intensity of standard depth
    wo: depth corresponding Ipo
    and the effective liver volume is
    V=Σ(s×wi)……………(3)
    s: area of metrix ROI
    In order to check the accuracy of this method, this was performed-the liver phantom, the dog's liver- and the patient's liver-scintigraphy. The volume obtained for the liver phantom was nearly equal to the real volume and the dog's was almost equivalent to the weight of it. The volume for every patient showed good correlation with the volume as measured by CT scan.
    Part 2
    Liver-scintigraphy were performed in 14 preoperative patients of hepatectomy. The estimated volume of residual liver and the total effective liver volume were evaluated, and the accumulation rates of Au-198 colloid in the total liver (K) and those in the estimated residual liver (K1) were determined. The operation was done and 3 weeks later the same examination was performed. The following calculation was studied as the index of resectable liver volume.
    estimated volume of residual liver × K1/total effective volume × normal K*
    * 0.184 (determined in our laboratory)
    The calculation values for two of 14 patients who developed liver failure after hepatic resection were 0.205 and 0.361, and for others without severe complications were more than 0.384. The results for small numbers of patients suggest that this calculation may be useful for determining the indications for hepatectomy.
    In regard to regeneration of the liver, only the ratio of the effective liver volume after right lobectomy (including extended right lobectomy) to it before appeared to be related to the K of preoperation.
  • 石部 陽二
    1982 年 94 巻 3-4 号 p. 235-246
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    The platelet count and platelet adhesiveness were examined and comparing with other parameters of inflammation in patients with rheumatoid arthritis (RA) and in adjuvant arthritic rats. The platelet count in 75 patients showed good correlation with the erythrocyte sedimentation rate (γ=0.41, p<0.01). The platelet adhesiveness of 40 RA patients tested by the glassbeads method showed a significantly increased retention rate compared with that of 16 normal controls (p 0.005). In RA patients, platelet adhesiveness was significantly greater in the strongly positive CRP group (CRP 5(+) or more) than in the weakly positive CRP group (CRP 1(+) and 2(+)) (p<0.005). In adjuvant arthritic rats, the platelet count and adhesiveness increased for 5 weeks after a one shot injection. Furthermore, good correlations were noted between the platelet count and platelet adhesiveness (γ=0.72, p<0.005), platelet count and paw size (γ=0.79, p<0.001), and platelet adhesiveness and paw size (γ=0.57, p<0.001). These data suggest that platelet count and adhesiveness would be one of the good parameters of inflammation in RA patients.
  • 石部 陽二
    1982 年 94 巻 3-4 号 p. 247-255
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    Plasma β-thromboglobulin (β-TG) concentration and platelet factor 4 (PF4) activity were examined and related to parameters of inflammation in patients with rheumatoid arthritis (RA). In 42 RA patients, the β-TG concentration assessed by radioimmuno-assay (β-TG RIA KIT Amersham) was significantly higher than that of 17 normal controls (p<0.002). The β-TG concentration correlated with the platelet count (γ=0.68, p<0.01) and erythrocyte sedimentation rate (γ=0.32, p<0.005). Plasma PF4 activity, tested by heparin neutralizing activity, was significantly higher than that of 17 normal controls (p<0.001). In the strongly positive CRP group (CRP 3(+) or more) PF4 activity was significantly higher than in the weakly positive CRP group (less than CRP 2(+)) (p<0.05). No correlation between PF4 activity and other parameters such as the platelet count, erythrocyte sedimentation rate, fibrinogen, antithrombinIII, or FDP was demonstrated. These results suggest that accerelated platelet functions were present in patients with clinically active RA.
  • 宇田 慎一
    1982 年 94 巻 3-4 号 p. 257-267
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    Serum, urine and synovial fluid β2-microglobulin (BMG) was measured by radioimmunoassay (Phadebas β2-micro test) in 43 rheumatoid arthritis (RA) patients being treated with gold salts, in 9 patients with osteoarthritis (OA) and in 9 patients with renal disease. The relationships between BMG, RA activity index and renal function were investigated. The serum BMG level in the renal disease group was significantly inversely correlated with creatinine clearance. The 43 rheumatoid arthritis patients were divided into a proteinuria group and a non-proteinuria group. The serum BMG level was higher in the renal disease group than in the proteinuria group, and was higher in the proteinuria group than in the non-proteinuria group. The synovial gluid BMG level was measured in 16 patients with RA and in the 9 patients with OA. This measure was higher in the RA group than in the OA group. The 43 RA patients were divided into positive and negative groups by C-reactive protein, RA test results (erythrocyte sedimentation rate over 50 mm/h and under 49 mm/h), and γ-globulin levels (over 1.5 g/dl and under 1.49 g/dl). Serum BMG levels were compared in these two groups. They tended to be higher in the higher activity groups than in the lower activity groups. From these results, a serum BMG level below 3.15 μg/ml (which is higher than in healthy normals) was considered “normal” for RA. The non-proteinuria group was investigated in a 2×2 table divided at 3.15 μg/ml serum BMG level and 500 μg/l urinary BMG level. There were two patients with abnormal BMG levels in both serum and urine samples, two patients with abnormal levels in the serum sample, and two patients with abnormal BMG levels in only the urine sample. It was thought that some patients without proteinuria in crysotherapy started to show renal damage. The results suggest that during gold treatment RA patients require not only urinalysis, but measurements of serum and urinary BMG levels for possible renal damage.
  • 宇田 慎一
    1982 年 94 巻 3-4 号 p. 269-287
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    Renal biopsies were performed on seven patients with rheumatoid arthritis (RA) and proteinuria. The material was examined by light and immunofluorescent microscopy. Two of the biopsy specimens were examined by electron microscopy. Membranous nephropathy was found in six of the seven specimens. Brilliant, fine granular fluorescence with IgG was present along the glomerular capillary loops under immunofluorescent microscopy. These findings suggested that these renal disorders were due to an immune complex mechanism. The proteinuria of five patients indicated that the membranous nephropathy occurred following D-penicillamine treatment or chrysotherapy. In one patient, there was no evidence of a relationship between D-penicillamine and proteinuria from her history. The remaining patient had a mild cell proliferative glomerulonephritis under light microscopy. Fluorescence was not found under immunofluorescent microscopy. For this reason, it seemed that the renal disorder was not caused by D-penicillamine, althoug her history showed that D-penicillamine brought on proteinuria. These findings suggested that renal biopsy is desirable in patients with long term proteinuria, when the proteinuria is clearly caused by D-penicillamine or gold salts. Furthermore, it seemed that long term proteinuria due to D-penicillamine, was probably caused by an immunological mechanism. The seven patients showed no evidence of secondary amyloidosis due to RA.
  • 仲宗根 浩二
    1982 年 94 巻 3-4 号 p. 289-300
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    A newly developed non-invasive and quantitative blood flow meter (QFM) was used to measure blood flow in human peripheral arteries (common carotid artery, femoral artery, dorsal pedal artery), and to determine hemodynamic differences after replacement of the canine abdominal aorta with a prosthesis.
    The results obtained were as follows:
    1) Blood flow of the canine femoral artery by QFM correlated well with that obtained by an electromagnetic flow meter with a correlation coefficient (r) of 0.94 (p<0.001) (y=1.05x-12.9).
    2) In normal subjects, blood flow of the common carotid, femoral and dorsal pedal arteries changed with age, namely, the blood flow of the common carotid artery decreased after the age of 40 with a statistically significant difference and that of the femoral artery after the age of 60.
    3) The blood flow and velocity in the femoral artery and dorsal pedal arteries increased markedly with exercise.
    4) The effect of the prosthetic valve in the mitral position on the aortic flow was determined in the common carotid artery. However, there was no significant difference.
    5) Blood flow and velocity increased after the aorta was replaced with a prosthesis, although there was no significant difference.
    It was concluded in the present study that non-invasiveness and the ability of QFM to detect fine changes in flow patterns would be an ideal tool for diagnosing and following up patients with vascular disease.
  • 山脇 泰秀
    1982 年 94 巻 3-4 号 p. 301-314
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    Until recently, it was widely accepted that neuroleptics act by blocking the dopamine receptor. Many investigators have done binding studies in vitro using [3H]-neuroleptics as ligands. However, recent studies on rat brains suggest that [3H]-neuroleptics label not only the dopamine receptor, but other receptors as well.
    In this study, the cross reactivities of numerous centrally acting drugs in dopamine-radioreceptor assay were different from those in spiroperidol-radioreceptor assay. Manganese enhanced the specific binding of [3H]-dopamine dramatically by 300% even at low concentrations, although manganese did not influence the specific binding of [3H]-spiroperidol. To differentiate dopamine and spiroperidol receptors in the rat brain, I solubilized these receptor molecules by treatment with detergent and examined the molecular weight by gel filtration. As compared with the elution volumes of marker proteins, the approximate molecular weight of solubilized dopamine receptors were about 200, 000 dalton (Stokes'radius: 5.0 nm) in the absence of manganese, and about 400, 000 dalton (Stokes'radius: 6.1 nm) in the presence of manganese. Moreover, manganese was found in the same fraction as the solubilized dopamine receptor. However, the molecular weight of the solubilized spiroperidol receptor was about 120, 000 dalton (Stokes'radius: 4.3 nm) in both the presence and absence of manganese.
    These data suggest that manganese couples specifically with manganese-free dopamine receptors, and that this manganese-dopamine receptor complex may have a strong capacity to bind dopamine. On the other hand, the receptor activities of spiroperidol were independent of manganese. These findings suggest that the site of action of spiroperidol is a receptor different from authentic dopamine receptor.
  • 山脇 泰秀
    1982 年 94 巻 3-4 号 p. 315-330
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    Long-term drug studies in animals using antiparkinsonian drugs (L-dopa and bromocriptine) and neuroleptics (chlorpromazine and haloperidol) can provide valuable clinical information on the mechanism of drug action and can contribute to a better understanding of the side effects of such therapy. Moreover, it has been suggested that the molecular action of these drugs involves dopamine receptors in the brain.
    Recently, it was proposed that the dopamine receptor exists in two forms: a D1-dopamine receptor, which is coupled to dopamine sensitive adenilate cyclase, and a D2-dopamine receptor, which is uncoupled. I have established D1-dopamine-radioreceptor assay and D2-dopamine-radioreceptor assay using [3H]-dopamine as a ligand, then I studied the effects of chronic administration of these drugs on two forms of dopamine receptors.
    It is well known that most patients on prolonged L-dopa therapy either become refractory to the drug, or develop “on-off” phenomena or dyskinesias. In this study, an increase in high affinity binding sites of D1-dopamine receptor was detected, whereas D2-dopamine receptor subsensitivity was observed following long-term L-dopa therapy. On the other hand, after bromocriptine treatment for 14 days, the high affinity binding sites of D1-and D2-dopamine receptors were increased in the rat striatum. The finding that long-term bromocriptine therapy increases D1-and D2-dopamine receptors may explain, in part, the efficacy of combination bromocriptine and L-dopa treatment.
    After chlorpromazine treatment for 14 days, the high affinity binding sites of D1-and D2-dopamine receptors were slightly increased in rat striatum, and after haloperidol treatment for 14 days, the high affinity binding sites of D2-dopamine receptor were also slightly increased in rat striatum. Therefore, it was suggested that symptoms of extrapyramidal side effects after long-term neuroleptic treatment reflect a functional supersensitivity of dopamine receptors in the rat striatum.
  • 西山 正一, 常盤 孝義, 佐藤 二郎
    1982 年 94 巻 3-4 号 p. 331-339
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    Cloned rat liver cells were treated with an ultimate carcinogen, N-acetoxy-methylaminoazobenzene(AcMAB) and 3'-methyl-4-dimethylaminoazobenzene(3'-Me-DAB) then various biological characters of the cells including tumorigenicity were examined. In regard to back-transplantation, after 11 days of experiments, a high concentration (5×10-4M) of AcMAB-treated cells formed tumors in one out of two rats. And a low concentration (1×10-4M) of AcMAB or 3'-Me-DAB-treated cells and untreated control cells did not form tumors. After 99 days of experiments, tumor formation was observed not only with the high concentration of AcMAB- or 3'-Me-DAB-treated cells but also with untreated control cells. AcMAB-treated cells had altered morphology and growth properties in vitro compared to 3'-Me-DAB-treated cells and untreated control cells. These results suggest an ultimate character of AcMAB, especially from the view point of tumor-forming capacity.
  • 松井 秀樹
    1982 年 94 巻 3-4 号 p. 341-348
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    1) The L-O cell absolutely required serum for proliferation, but L-N cell grew rapidly in serum free medium.
    2) L-N cell cultured in MEM medium with TPB reached high saturation density as in serum containing medium.
    3) The growth rate of L-N cell in serum free medium was dependent on initial density of cell population just before logarithmic growing phase.
    4) L-N cell rounded cell cycle from G-1 phase to S phase 4 hours faster than L-O cell.
  • 橋本 啓二
    1982 年 94 巻 3-4 号 p. 349-357
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    The 2-nitroso-(N-propyl-N-sulfopropylamino)phenol(Nitroso-PSAP), one of the watersoluble derivatives of nitrosoaminophenol, is a very sensitive reagent for the spectrophotometric determination of Fe (II). Using Nitroso-PSAP reagent, the author investigated the conditions for determining Fe (II) quantitatively and selectively the system of Fe (II)-induced lipid peroxidation, and obtained the following results.
    1) Fe (II) could be measured quantitatively without reductant. The Fe (II) complex had a molar absorptivity of 4.5×104 dm3 mol-1 cm-1 at 756 nm.
    2) Fe (II) could be measured quantitatively in the TCA solution removed from the reaction medium for lipid peroxidation. The Fe (II) complex had a molar absorptivity of 3.14×104 dm3 mol-1 at 756 nm.
    3) Fe (II) could be measured quantitatively and selectivily under conditions involving a mixture of Fe (II).
  • 橋本 啓二
    1982 年 94 巻 3-4 号 p. 359-368
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
    The author measured simultaneously Fe (II)-induced peroxidation of lipid extracted from rat liver mitochondria by the TBA method and the concentration of Fe (II) added to the medium quantitatively using Nitoroso-PSAP reagent.
    1) An induction period (lag) at the early phase of the reaction of Fe (II)-induced lipid peroxidation was observed as well as in mitochondria.
    2) After most of the Fe (II) concentration had decreased, the TBA-value, after lag-time, increased sharply.
    3) Phosphate added to the medium shortened the lag-time. The lag-time in the presence of phosphate varied with the concentration and pH. However, in each case, there was a correlation between the decrement of Fe (II) concentration and the length of lag time as described above.
    4) By adding EDTA for various reaction times, Fe was chelated immediately, then the TBA value did not increase.
    5) The results suggest: iron interacted with the chain reaction of lipid peroxidation, mainly as Fe (II) and/or partially Fe (III); Fe (II) maintains the lag as a inhibitory action to the resolution into malondialdehyde(MDA) from lipid peroxide; Fe (III) was required for the formation of MDA.
    6) Using these results, the author discussed the mechanisms of Fe (II)-induced lipid peroxidation.
  • 1982 年 94 巻 3-4 号 p. 369-376
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
  • 1982 年 94 巻 3-4 号 p. 377-384
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
  • 1982 年 94 巻 3-4 号 p. 385-392
    発行日: 1982/04/30
    公開日: 2009/03/30
    ジャーナル フリー
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