巻号一覧
71 巻 (1959)
- 12-2 号 p. 8243-
- 12-1 号 p. 7861-
- 11supplement 号 p. 1-
- 11-2 号 p. 7531-
- 11-1 号 p. 7095-
- 10-2 号 p. 6691-
- 10-1 号 p. 6223-
- 9-2 号 p. 5773-
- 9-1 号 p. 5405-
- 8-2 号 p. 4993-
- 8-1 号 p. 4515-
- 7-2 号 p. 4069-
- 7-1 号 p. 3623-
- 6-2 号 p. 3149-
- 6-1 号 p. 2783-
- 5-2 号 p. 2423-
- 5-1 号 p. 1989-
- 4-2 号 p. 1655-
- 4-1 号 p. 1145-
- 3supplement-2 号 p. 1-
- 3supplement-1 号 p. 1-
- 3-2 号 p. 1145-
- 3-1 号 p. 691-
- 2-2 号 p. 691-
- 2-1 号 p. 323-
- 1 号 p. 1-
前身誌
99 巻, 5-6 号
選択された号の論文の27件中1～27を表示しています
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岡崎 悟1987 年 99 巻 5-6 号 p. 423-430
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーTo clarify on a cellular basis the mechanism of the diabetogenic effect of the westernized diet, insulin binding, insulin stimulated 3-o-methylglucose uptake and glucose oxidation were studied in isolated adipocytes from rats fed experimental diets: low fat-no sugar diet (energy ratio of 10% fat, 70% starch, a model of the traditional Japanese diet), high fathigh sugar diet (40% fat, 20% starch, 20% sugar, a model of the westernized diet), low fat-high sugar diet (10% fat, 50% starch, 20% sugar) and high fat-no sugar diet (40% fat, 40% starch).
In high fat-high sugar diet rats, epididymal fat and mean fat cell size were significantly greater than in low fat-no sugar diet rats. There was no significant difference in insulin binding and insulin stimulated 3-o-methylglucose uptake between high fat-high sugar diet rats and low fat-no sugar diet rats. However, insulin stimulated glucose oxidation was markedly lower in high fat-high sugar diet rats. Therefore, it is suggested that the high fat-high sugar diet induced a post-receptor defect but did not influence receptor binding in rat adipocytes.
In high fat-no sugar diet rats, a similar post-receptor defect was found, but in low fat-high sugar diet rats, insulin binding and post-receptor insulin action were unchanged.
In conclusion, the high fat-high sugar diet may produce diabetogenic effects through reduced insulin action due to a postreceptor defect.抄録全体を表示PDF形式でダウンロード (390K) -
915 MHzマイクロウェーブ照射による脳加温効果の検討松本 健五1987 年 99 巻 5-6 号 p. 431-442
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーDifferential hypothermia (D.H.), in which tumors are kept normothermic under total body hypothermia, has been confirmed to have a therapeutic effect. It is essential in the treatment of malignant brain tumors by D.H. to deliver the heat uniformly to specific volumes of tissue and to maintain the optimal temperature for an appropriate period of time.
A new instrument for delivering electromagnetic radiation at 915 MHz was devised, and its therapeutic efficacy was evaluated in comparison with conventional 2, 450 MHz microwave diathermy. Adult canine or monkey brains were exposed to microwave irradiation following fronto-parietal craniectomy under total body hypothermia (29±1°C).
The highest temperture in the brain was controlled manually to be between 3-37°C. The average temperature at depths of 0, 0.5, 1.0, 1.5, 2.0 and 3.0 cm was 34.8°C, 35.5°C, 36.0°C, 36.7°C, 34.4°C, and 30.7°C, respectively, at 915 MHz ang 3.8°C, 35.4°C, 36.0°C, 3.°C, 34.5°C, and 32.0°C, respectively, at 2, 450 MHz. Deeper heat penetration was achieved with 915 MHz than with 2, 450 MHz MW irradiation. The temperature of the brain was controlled easily and could be kept 7-10°C higher than that of the cooled body.
Heat toxicity was examined histopathologically following a 5-hour-D.H. treatment. No brain damage was observed in brains heated to 37°C, but irreversible changes such as exudative homorrhage were revealed in brains kept at 40°C.
These results suggest that: 1) 815 MHz MW irradiation can effectively induce localized brain hyperthermia. 2) Deeper heat penetration was achieved by 915 MHz than 2, 450 MH MW irradiation. 3) Temperatures above 40°C for 5 hours induced irreversible changes in the brain under total body hypothermia (29±1°C).抄録全体を表示PDF形式でダウンロード (1526K) -
堀井 茂男1987 年 99 巻 5-6 号 p. 443-465
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーTo evaluate the therapeutic effect of Naikan psychotherapy for alcohol dependence syndrome, a comparison was made between 31 patients who were treated with Naikan psychotherapy (Naikan group) and 34 patients who were not treated with Naikan psychotherapy (non-Naikan group) on the following profiles: general characteristics, social adaptation occuring 6 months to 2 years 6 months after discharge (short-term follow-up) and social adaptation occuring 3 years 5 months to 5 years 5 months after discharge (long-term follow-up). Some typical cases of Naikan psychotherapy were presented.
It was suggested that Naikan psychotherapy was effective for the treatment of alcohol dependence syndrome. It was useful in helping the alcoholics to understand the necessity of abstaining (motivation to abstain) and had a long-term therapeutic effect (maintenance of abstinence). It was concluded that the therapeutic effect of Naikan psychotherapy was derived from the understanding of egocentricity by acquiring a therapeutic guilt-feeling and the overcoming the denial in the alcoholic patient.抄録全体を表示PDF形式でダウンロード (2156K) -
福田 哲也1987 年 99 巻 5-6 号 p. 467-475
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーTo investigate the interrelationships between the westernized diet and physical exercise as they affect the development of non-insulin-dependent diabetes mellitus (NIDDM), adiposity, glucose tolerance and insulin response to an intraperitoneal glucose load (1.5g/kg bw) and insulin sensitivity to exogenous insulin (0.2U/kg bw) were studied in spontaneously exercised and sedentary rats fed either a high fat diet (40% fat, modern western type) or a low fat diet (10% fat, traditional Japanese type).
The high fat diet, compared with the low fat diet, increased the deposition of epididymal fat pads in exercised and sedentary rats. Physical exercise decreased the deposition of fat pads in the high and low fat diet rats. The high fat dier, compared with the low fat diet, increased glucose-stimulated glucose and insulin levels in the exercised and sedentary rats. Physical exercise led to reductions in glucose-stimulated glucose and insulin levels in the high and low fat diet rats. The high fat diet, compared with the low fat diet, decreased insulin sensitivity to exogenous insulin in the exercised and sedentary rats. Physical exercise increased insulin sensitivity in the high and low fat diet rats. Physical exercised rats fed the high fat diet and the sedentary rats fed the low fat diet had simular adiposity, glucose tolerance and insulin sensitivity.
These data indicated that both the high fat diet and lack of physical exercise strongly affect the development of NIDDM.抄録全体を表示PDF形式でダウンロード (490K) -
本田 千穂1987 年 99 巻 5-6 号 p. 477-491
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーThe author analyzed water-soluble proteins of cultured human and rat glioma cells obtained by freezing and thawing cells suspended in distilled water. The author used O‘Farrell’s two dimensional polyacrylamide gel electrophoresis (NEPHGE method) with slight modification to analyze differences in the protein map, and Manabe's microscale two dimensional electrophoresis without denaturing agents for Western blotting to detect proliferating cell nuclear antigen (PCNA/cyclin). By O‘Farrell’s method and the silver staining technique, at least 200 different polypeptides were clearly identified in each cell line. Cytoskeletal proteins such as actin were constantly separated in all cell lines. Marked differences in the protein map were observed between human and rat glioma cell lines, and even within the same species. It is supposed that these differences arise from the cell-biological differences between the glioma cell lines. Marked differences in the portion map were also seen between proliferating glioma cells and glioma cells treated with forskolin (an adenylate cyclase activator), which can induce morphological and biochemical differentiation of glioma cells. Some proteins increased in amount after the treatment with forskolin, suggesting that these proteins are related to the differentiation of glioma cells. Some other proteins which were prominent in the proliferating cells diminished in cells cultured 24 hours in medium without calf serum to suppress cell growth. PCNA (proliferating cell nuclear antigen), an acidic nuclear protein which appears solely in late G1-S phase and is believed to be closely related to cell proliferation, was revealed by Western blotting and indirect immunostaining. The quantitative investigation of the PCNA spot on the protein map may be useful in assessing the proliferating activity of glioma cells. These results indicate that two dimensional polyacryamide gel electrophoresis is useful for the clarification and understanding of the biological features of glioma cells.抄録全体を表示PDF形式でダウンロード (2705K) -
佐伯 晋成1987 年 99 巻 5-6 号 p. 493-499
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーIsoflurane was administered to eight patients initially in a semiclosed inhalation system for 30 min., and then in a closed system for 150 min. Isoflurane was administered according to a pharmacokinetic model in which the muscle was postulated to take up most of anesthesia. The blood isoflurane concentrations of the patients were measured every 15 min. during closed system anesthesia, and they remained constant. The application of the pharmacokinetic model to the semiclosed-to-closed anesthetic system is thought to be very useful.抄録全体を表示PDF形式でダウンロード (311K) -
高倉 範尚1987 年 99 巻 5-6 号 p. 501-513
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーPancreatic oncofetal antigen (POA) is generally accepted as a useful tumor marker for the detection of cancer of the pancreas. However, reports on the biochemical characterization of POA have been contradictory. In the present study, biochemical and immunohistological characterizations of POA were performed with anti-fetal pancreas antibody specific to POA.
POA of the fetal pancreas was located in the region of α2β on immunoelectrophoreto-grams, and the molecular weight was estimated to be about 200, 000 by gel filtration. POA did not react with anti-CEA, AFP, or ferritin antibodies in the Ouchtalony test. POA was also detected in extracts of cancerous pancreas by Ouchtalony test. From immunohistological studies with anti-POA serum, it was confirmed that POA was present in the cytoplasm of fetal and cancerous pancreas. Well differentiated tumor cells stained much more than poorly differentiated tumor cells.
In conclusion, cancerous pancreas shares the same antigen, POA, with fetal pancreas, and the production of POA in cancerous pancreas is related to the degree of tumor cell differentiation.抄録全体を表示PDF形式でダウンロード (1671K) -
白神 敏明1987 年 99 巻 5-6 号 p. 515-527
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーThe serum and urinary ferritin levels and peripheral blood or synovial fluid leukocyte ferritin contents of 76 patients with rheumatoid arthritis (RA) were measured by the 2-site immunoradiometric assay method. Serum ferritin (s-fer.) levels of RA patients (mean±SD=33.9±26.3 ng/ml, n=45) were within the normal range, although the s-fer. level correlated well with the amount of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), γ-globulin level, RA test and disease activity. Urinary ferritin (u-fer.) levels of RA patients (8.7±12.7 ng/ml, n=45) were not significantly different from controls (5.1±4.1 ng/ml, n=23). However, when RA patients were divided into groups according to the therapy received at time of measurement, u-fer. level of RA patients under gold therapy (14.1±20.2 ng/ml, n=15), but not those of patients under other treatments, were significantly higher than control level. The U/S ratio also tended to be higher. In addition, a remarkably high u-fer. level (over 300 ng/ml) and U/S ratio (5.84) were found in are RA patient with gold nephropathy, indicating ferritin synthesis in kidney tissue.
Ferritin contents of the supernatants of leukocytes were measured. Synovial fluid leukocytes (PMN: 29.7±9.5 fg/cell (n=16); MNC: 62.4±7.1 fg/cell (n=16)) tended to have higher ferritin contents than peripheral blood leukocytes (PMN: 5.3±2.6 fg/cell (n=16); MNC: 9.3±3.3 fg/cell (n=16)). There was no significant correlation between the ferritin contents of PMN or MNC of peripheral blood or synovial fluid and other parameters such as ferritin levels, ESR, CRP or RA test, except for a positive correlation between the ferritin contents of peripheral blood PMN and serum iron levels.抄録全体を表示PDF形式でダウンロード (978K) -
第2報 濾紙CBB-G250色素法を用いた尿微量蛋白の新定量法宮井 泰三1987 年 99 巻 5-6 号 p. 529-536
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーA simple and sensitive method for rapid semiquantitative and quantitative determinations of urinary protein was devised, in which protein absorbed and fixed on filter paper containing sulfosalicylic acid was reacted with Coomassie Brilliant Blue G-250 (CBB). For the semiquantitative method, filter paper (NO. 50, Toyo) was immersed in a 20% sulfosalicylic acid solution and dried. Dried filter paper was immersed in aurine, dried again immersed TONEIN (Othuka Assay Laboratories) and compared with standard filter paper by the naked eye. For the quantitative method, filter paper was immersed in a mixture of ethanol, acetic acid and distilled water (35:10:55) containing 0.2% CBB, and washed water untill the waste water became colorless. The paper was again immersed into the mixture but without CBB, after which the paper was rinsed with water again. The protein concentration in the urine sample was determined by comparing the hue of the urine with a standard violet hue chart by the naked eye, or the color intensity of the extract of 1 cm2 of filter paper with 3% hydrogen chloride in ethanol was determined at 590 nm.抄録全体を表示PDF形式でダウンロード (1709K) -
第1編 即時型アレルギー反応におけるheparinの作用機序に関する研究小野 波津子1987 年 99 巻 5-6 号 p. 537-546
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーThe action of heparin on allergic reactions was examined in skin and bronchial reactions to chemical mediators, in the rat PCA reaction and antigen-induced degranulation of rat peritoneal mast cells. Heparin inhibited the histamine-induced skin reaction in rats. Bronchoconstriction caused by histamine and acetylcholine was also inhibited by pre-treatment with heparin. The skin reaction due to reaginic antibody (PCA), but not that due to precipitated antibody, in rats was inhibited by heparin administration. Heparin moderately inhibited the degranulation of rat peritoneal mast cells elicited by reaginic antibody, but the inhibitory action was not as strong as that of prednisolone or disodiumcromoglycate.抄録全体を表示PDF形式でダウンロード (1170K) -
第2編 気管支喘息における血漿heparinに関する研究小野 波津子1987 年 99 巻 5-6 号 p. 547-557
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーTo observe the participation of heparin in asthma attacks, plasma concentrations of heparin were estimated in asthmatic subjects. Changes in the plasma concentration of heparin after intravenous administration were also examined during asthma attacks. The mean plasma concentration of heparin was 0.017 u/5 ml in healthy subjects, and 0.017 u/5 ml in the non-attack stage, 0.018 u/5 ml in the pre-attack stage and 0.016 u/5 ml in the attack stage of bronchial asthma patients. The plasma concentration of heparin in the mild cases of asthma was higher in the attack-free period than in the attack period, while the plasma concentration of heparin in the severe cases of asthma was significantly lower in the attackfree period than in the attack period. There was a significant correlation between the plasma concentration of heparin and the peripheral basophil count. The time-dependent reduction in plasma heparin after intravenous administration was more remarkable in asthmatic subjects than in healthy subjects, and the reduction was most remarkable in the large attack stage of asthma.
The half-reduction time of the plasma heparin concentration after intravenous administration was 1 h and 20 min.抄録全体を表示PDF形式でダウンロード (547K) -
第3編 気管支喘息におけるheparin療法小野 波津子1987 年 99 巻 5-6 号 p. 559-567
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーSeventy-five patients with bronchial asthma were treated with heparin by three different administration methods: 5000 u intravenously plus 5000 u by inhalation (method 1), 5000 u intravenously plus 5000 u by inhalation combined with 1 mg sulbutamol (method 2) and 10000 u by inhalation (method 3).
The percent efficacy of heparin therapy was 44.4% in the bronchial asthma patients treated by method 1, 72.7% in those treated by method 2 and 25.0% in those treated by method 3. Heparin therapy was more effective in patients over 50 years old, those with an age at onset of 40 years or more (late onset asthma) and those with serum IgE levels of less than 400 u/ml. The efficacy of heparin was also observed more frequently in cases with lower FEY 1.0%, V50 and V25 values. Reduction of the plasma heparin concentration 1 h after intravenous administration was more rapid in cases in which heparin was effective than in those in which it was not.抄録全体を表示PDF形式でダウンロード (803K) -
井口 郁雄, 増田 游, 高塚 ま由, 小倉 義郎, 藤本 明子1987 年 99 巻 5-6 号 p. 569-575
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーHearing loss in children under school age adversely effects speech and personality development. It is possible to improve conductive hearing loss by surgery, but difficult to improve combined hearing loss. The authors succeeded surgically improving the hearing of a 5 year-old boy suffering from speech retardation due to bilateral congenital combined hearing loss. The improvement in hearing aided speech training. He has graduated from schools for the deaf (primary, middle and senior high school). His I.Q. is 70 or less. The average hearing is in the speech range of 90 dB bilaterally, showing combined hearing loss by play audiometry. At the age of 1.5 years, he was suspected of having congenital aural atresia on the right side, congenital narrow ear canal on the left side and minor anomalies of the auricles bilaterally, including congenital aural fistulas. His mother and younger brother also suffer from bilateral congenital combined hearing loss.
The tympanotomy on the left ear revealed severe anomalies, such as omega shaped ossicle composed of the malleus and incus joined to the posterior bony wall of external auditory canal and bony fixation of the stapes footplate. The bulky ossicle was mobilized by cutting the junction to the bony canal wall, and a small fenestra stapedectomy was performed.
A tympanotomy on the right ear was also performed, but irregular development of the inner ear prevented the possibility of obtaining hearing improvement.
The authors discussed the possibility and significance of surgery to improve hearing even in cases of profound combined hearing loss in preschool children.抄録全体を表示PDF形式でダウンロード (2215K) -
大谷 裕子, 北川 紀典1987 年 99 巻 5-6 号 p. 577-581
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーThis paper reports the first case of tsutsugamushi disease (scrub typhus) occurred in Okayama prefecture. The present case was characterized by: 1) arthralgia, conjunctivitis, cough, headache and intermittent fever with malaise, 2) an ulcer (10×7 mm) covered with reddish black eschar, which developed at the inoculation site on the middle of the anterior border of the left axilla, and swelling of the lymphnodes, 3) rash on the whole body, which blanched on pressure, and 4) a positive reaction to the test for C-reactive protein (CRP). Administration of piperacillin (1g/day, for 2 days) was not effective at all, but that of minocycline (200 mg/day, for 1 day, followed by 100 mg/day, for 7 days) was so effective that body temperature rapidly fell to normal within a day. The general condition improved promptly. The indirect immunoperoxidase reaction showed a high titer of serum antibody for the Karp type of Rickettsia tsutsugamushi. Since Rickettsia tsutsugamushi is distributed widely in Japan, including Okayama prefecture, the physician should take tsutsugamushi disease into consideration when diagnosing acute feverish illnesses.抄録全体を表示PDF形式でダウンロード (746K) -
第1編 ヒト肥満細胞の抗凝固活性について津田 隆史1987 年 99 巻 5-6 号 p. 583-594
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーThe anticoagulant activity of proteoglycans released from human lung mast cells was evaluated. Human lung mast cells were mechanically and enzymatically dissociated from normally appearing human lung fragments obtained during surgery for carcinoma and enriched by velocity gradient sedimentation. Mast cells ((1.00±0.31)×107) were obtained with a purity of 20.1±3.3%. Isolated human lung mast cells, which were passive-sensitized with high IgE serum from an allergic patient and challenged with rabbit anti-human IgE or stimulated by calcium ionophore A23187, released proteoglycans which stained metachromatically with Azure A, as well as histamine. Both anti-thrombin activity (33.8±5.8%) and anti-Factor Xa activity (28.1%) were detected in proteoglycans released from human lung mast cells by the amidolytic method using chromogenic substrate S-2238 and S-2222, respectively. A significant correlation between anti-thrombin activity and the metachromatic change with Azure A was found. The metachromatic materials with anticoagulant activity were identified to be heparin by the criteria that they were resistant to degradation by chondroitin ABC lyase and chondroitin AC lyase, completely degraded by heparinase and inhibited by Polybrene. These data suggest that heparin proteoglycan released from human lung mast cells in an IgE mediated hypersensitivity reaction may prevent deposition of fibrin and play an important role at inflammatory lesions.抄録全体を表示PDF形式でダウンロード (697K) -
第2編 ヒト好塩基球の抗凝固活性について津田 隆史1987 年 99 巻 5-6 号 p. 595-606
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーThe anticoagulant activity of proteoglycans released from human basophils was evaluated. Basophils were obtained from normal donors and patients with abnormally high peripheral blood basophilia accompanying chronic myelogenous leukemia (CML) and enriched by Hypaque-Ficoll gradient sedimentation. From normal donors (8.9±4.6)×106 basophils were obtained with a purity of 6.2±1.7%, and from patients with CML (1.4±0.3)×107 basophils were obtained with a purity of 52.1±6.9%. Isolated basophils from normal donors, which were passive-sensitized with high IgE serum from an allergic patient and challenged with rabbit anti-human IgE or stimulated by calcium ionophore A23187, released proteoglycans which stained metachromatically with Azure A, as well as histamine. No anticoagulant activity was detected in proteoglycans released from human basophils by the amidolytic method using chromogenic substrate S-2238. Isolated basophils from patients with CML, which were labeled by [35S] sulfate, were also passive-sensitized and challenged with rabbit anti-human IgE. Released [35S] proteoglycans filtered on Sephadex G-25 were resistant to heparinase and chondroitin-6-sulfatase, and susceptible to degaradation by chondroitin ABC lyase, chondroitin AC lyase and chondroitin-4-sulfatase. These findings indicate that the [35S] proteoglycans released from basophils were made up of chondroitin sulfate type A. No heparin was identified. The basophil proteoglycans had no anticoagulant activity and were different from the proteoglycans of human lung mast cells. This fact may be particularly relevant to the location of each cell.抄録全体を表示PDF形式でダウンロード (719K) -
寺坂 律子1987 年 99 巻 5-6 号 p. 607-622
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーVectorcardiograms obtained with the Frank lead system were recorded in 258 healthy subjects (139 men, 119 women) and were classified according to QRS loop patterns. Sex and age effects on the vectorcardiograms were studied. We classified the vectorcardiograms into 4 types according to the frontal axis of the maximum QRS vector (A60>B30>C10>D). Type B was frequent among young persons and the number of persons with type C increased with aging. The magnitude of the maximum QRS vector diminished with aging in both sexes. The axis deviated horizontally and posteriorly in men and horizontally in women. The magnitude of the maximum T vector was larger in men than in women. The axis was more anterior in men than in women. The axis of the 20-msec QRS vector deviated from the right anterior to the left anterior. The terminal QRS vector demonstrated no sex or age defference. The percentage of the QRS Bite increased with aging.抄録全体を表示PDF形式でダウンロード (1200K) -
第1編 Bestatin[3-(R)-amino-2-(S)-hydroxy-4-phenylbutanoyl-(S)-leucine]のcyclophosphamide誘発造血抑制からの回復促進効果内田 耕三郎1987 年 99 巻 5-6 号 p. 623-632
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーThe hemopoietic potential of Bestatin, a new immunomodulator prepared from the culture medium of Streptomyces olivoreticuli, was studied concerning its effects on the recovery from cyclophosphamide(CPA)-induced myelosuppression and on autologous CFUs in irradiated mice. In mice treated with CPA (300 mg/kg, i.p., day 0), the recovery from granulocytopenia and myelosuppression was significantly accelerated by administration of Bestatin (0.05 mg/kg, i.p., -day 5 to -day 1). Bestatin, administrated i.p. at a dose of 0.05 mg/kg on -day1, increased the number of autologous CFUs in irradiated mice, indicating that Bestatin acted at the level of multipotent stem cells. These result indicate that Bestatin may be useful in the clinical management of myelosuppression induced not only by cancer chemotherapy but also by radiation therapy.抄録全体を表示PDF形式でダウンロード (1828K) -
第2編 諸種免疫賦活剤[Bestatin, 溶連菌製剤OK-432, 有機化合物Carboxyethylgermanium Sesquioxide (Ge-132)]による造血能賦活効果の比較検討内田 耕三郎1987 年 99 巻 5-6 号 p. 633-642
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーThe hemopoietic potentials of three immunomodulators, Bestatin, OK-432 and Ge-132, were compared in terms of the effects of the drugs on the recovery from cyclophosphamide (CPA) induced myelosuppression, and on autologous CFUs in irradiated mice. The recovery from CPA-induced myelosuppression was significantly accelerated in mice pretreated with Bestatin or OK-432 as compared to mice pretreated with Ge-132 or physiological saline. The effect of OK-432 was somewhat superior to that of Bestatin, but there were no significant differences in the days to nadir and in the days required for the recovery from nadir. Both Bestatin and OK-432 significantly increased the number of autologous CFUs in irradiated mice as compared to Ge-132 and Lentinan. Most chemotherapeutic schedules are divided into four therapeutic phases: induction, consolidation, maintenance and intensification. The last two phases were usually applied to outpatients; therefore, hemopoietic agents, such as Bestatin, which can be orally administrated, may be useful clinically for the prevention of chronic myelosuppression during maintenance and intensification chemotherapy.抄録全体を表示PDF形式でダウンロード (1038K) -
西崎 進1987 年 99 巻 5-6 号 p. 643-655
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーThe systemic and hepatic circulation dynamics of 20 mongrel dogs were investigated both during and after anesthesia with oxygen and 1% or 2% halothane (OF 1% or OF 2%). A decrease in the systolic force during halothane inhalation was accompanied by contraction of peripheral blood vessels under OF 1%, but was accompanied by dilatation of these vessels under OF 2%. The hepatic arteries were not influenced by the anesthetic depth, while the mesenteric blood vessels contracted under halothane inhalation. The peripheral blood vessels contracted under OF 1% but dilated under OF 2%. As the anesthetic depth increased, first the mesenteric blood flow decreased, followed by a decrease in the hepatic arterial flow proportional to the decrease in the blood pressure. The cardiac output and peripheral vascular resistance were reduced, so that the blood flow was redistributed to the superficial and muscular blood vessels, leaving the organs in a state of hypoxia. As the hepatic arterial blood flow is influenced by the blood pressure, the hepatic circulation becomes unfavorable when the blood pressure drops under halothane inhalation. For this reason, it is important that the blood pressure not be allowed to fall too much during halothane anesthesia. Another problem with halothane anesthesia is that the blood flow to the organs remains reduced for at least 2 h after termination of the inhalation, thus subjecting the abdominal organs to hypoxia. This condition may lead to liver damage as a postoperative side effect of halothane anesthesia. This tendency was clearly observed under deep anesthesia, which suggests that deep anesthesia with halothane should be avoided.抄録全体を表示PDF形式でダウンロード (695K) -
第一編 オボムコイドを基質とした測定法の基礎的検討香川 俊介1987 年 99 巻 5-6 号 p. 657-665
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーThe conditions assaying for UDP-galactosyltransferase in human gastric mucosa was studied. Ovomucoid without any pretreatment was employed as a substrate. The optimal pH was 6.8. Manganese, 2-mercaptoethanol and Triton X-100 were required for maximum enzyme activity. In the standard assay; 20 μl of enzyme solution was added to 30 μl of 50 mM MES buffer containing 5 mM 2-mercaptoethanol, 15 mM MnCl2, 9.9mg protein/ml ovomucoid, 0.3 mM UDP-galactose, 1.7 μCi/ml UDP-[3H]-galactose and 1.5 mg/ml Triton X-100, and incubation was carried out at 37°C for 20 min. [3H]-labelled ovomucoid was the only radioreactive reaction product detected. All galactose residue incorporated was liberated by β-galactosidase but not by α-galactosidase.抄録全体を表示PDF形式でダウンロード (817K) -
第二編 胃潰瘍症例における検討香川 俊介1987 年 99 巻 5-6 号 p. 667-672
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーBiopsied specimens of gastric mucosa of normal subjects and patients with gastric ulcer were obtained under routine gastrofiberscopy. UDP-galactosyltransferase activity was measured with the use of ovomucoid as a substrate. In endoscopically normal subjects, UDP-galactosyltransferase activity of the fundic gland area was significantly (p<0.01) higher than that of the pyloric gland area. In ulcer patients without anti-ulcer treatment, UDP-galactosyltransferase activity was elevated in the mucosa surrounding the ulcer at the healing stage and red scarring stage as compared with the corresponding area of non-ulcer individuals. However, under administration of an H2-blocker, the change in UDP-galactosyltransferase activity was not observed. These observations may suggest that (1) increased glycoprotein synthses may be a physiological response of the gastric mucosa to the ulcer and may promote ulcer healing, and that (2) the process of the H2-blocker induced ulcer healing may not be associated with the apparent increase in glycoprotein synthesis.抄録全体を表示PDF形式でダウンロード (299K) -
山崎 弘子1987 年 99 巻 5-6 号 p. 673-679
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーThe cimetidine concentration of serum and urine was measured by high-performance liquid chromatography (HPLC). Using procaine hydrochloride as an internal standard, samples were applied to a μ-Bondapak C18 column with acetonitrile/10 mM potassium phosphate buffer, pH 4.8, (7/93 by volume) as the mobile phase. The recovery with this method was 62.6% for serum and 65.0% for urine. The quantitative determination of cimetidine in serum and urine can be performed from 0.1 mg/l to 500 mg/l and from 6.25 mg/l to 2000 mg/l, respectively. For this determination, only 200 μl of serum or urine were required. The column for separation of cimetidine was found to be stable for at least 8 months (1, 300 samples). This simple method is suitable for routine measurement of cimetidine in serum and urine.抄録全体を表示PDF形式でダウンロード (322K) -
池田 滋樹1987 年 99 巻 5-6 号 p. 681-690
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーDSCG, EAAおよびtranilastのhistamine遊離抑制作用の様式を明らかにする目的で,マウス抗卵白アルブミン血清で受身感作したラット腹腔肥満細胞からのアナフィラキシー性histamine遊離に対するこれらの薬物の効果を調べた.
DSCGはCa2+を含む媒液中では濃度依存的な強力なhistamine遊離抑制活性を示したが, Ca2+を添加しない媒液中では,活性はCa2+存在下での活性と比較してはるかに弱かった. EAAとtranilastの活性には細胞外Ca2+の存否による明瞭な差異が認められなかった.
DSCG, EAAおよびtranilastのhistamine遊離抑制効果は薬物添加から抗原添加までの時間の延長に伴って漸次減弱した(タキフィラキシーの発現).
三種類の薬物のうち一つに対してタキフィラキシー状態となった肥満細胞では,他の薬物の効果も著明に減弱していた(交叉タキフィラキシーの成立).
以上の結果より,ラット肥満細胞表面の特異的部位へのDSCGの最適結合には,細胞外Ca2+が必要であるが, EAAおよびtranilastの肥満細胞との相互作用は細胞外Ca2+が存在しなくても起こり得るものと推測される.しかし,これら三種類の薬物間に交叉タキフィラキシーが成立することから,これらの薬物の作用様式には共通性があり,肥満細胞表面の同じ特異的部位と結合する可能性が考えられる.抄録全体を表示PDF形式でダウンロード (562K) -
第1編 ヒトintermediate cell type肺小細胞癌細胞株(SBC-1)に対するモノクローナル抗体の作製と解析中村 邦彦1987 年 99 巻 5-6 号 p. 691-700
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーFour IgM monoclonal antibodies against an intermediate cell type small cell lung cancer (SCLC) cell line (SBC-1) were produced and characterized. Reactivities of the antibodies to cell lines were tested by membrane immunofluorescence, and the reactions with normal and neoplasma tissues were analyzed using an indirect immunoperoxidase technique. Each of the 4 antibodies reacted with lung cancer cell lines and lung cancer tissues, but did not exhibit lung cancer histology-type specificity. The 4 antibodies reacted with normal bronchiolar and digestive gland tissues, and the antibody-reactive antigens were shown to be a gland-tissue differentiation antigen. The antigen was expressed on O type red cells, was not expressed on other erythrocytes and was undetectable in intermediate SCLC tissues. This may represent the epitope-difference of the antigen recognized by the antibodies. None of the antibodies reacted with oat SCLC, but intermediate and non-small lung cancer reacted with the antibodies. This result shows the antigen-similarity of intermediate SCLC with non-small lung cancer.抄録全体を表示PDF形式でダウンロード (1416K) -
第2編 肺癌関連モノクローナル抗体6種の免疫組織化学的解析と喀痰細胞診への応用中村 邦彦1987 年 99 巻 5-6 号 p. 701-713
発行日: 1987/06/30
公開日: 2009/03/30
ジャーナル フリーImmunohistochemical reactivities of six lung cancer-associated monoclonal antibodies to the tissue sections of lung cancer, other neoplasms and normal tissues and to the Saccomanno's treated cells were analyzed using an indirect immunoperoxidase technique.
The antibodies included LA-4 raised against a lung adenocarcinoma, LSO-4 and -5 against an oat cell lung cancer, LSI-a and -2 against intermediate small cell lung cancers and LE-3 against an epidermoid lung cancer. Six fixation methods were compared, and PFA fixation revealed not to change the antigenisity detected by the antibodies.
LSO-4 and -5 antigens were completely dissappered by paraffin-embeded and Saccomanno's treatment. LA-4, LSI-a and -2 were reacted with normal bronchioles and lung cancer tissues. These normal differentiation antigens were expressed on the tissues of lung adenocarcinoma and small cell and epidermoid lung cancers. LE-3 antibody reacted with normal platlets and large intestine tissues, and the LE-3-reacted antigen expressed not only on lung epidermoid cancer but on lung adenocarcinoma. LE-3 did not react with normal lung tissues or Saccomanno's treated normal cells, but reacted the Saccomanno's treated epidermoid lung cancer cells, thus appered to be useful for sputum cytology.抄録全体を表示PDF形式でダウンロード (1991K) -
1987 年 99 巻 5-6 号 p. 715-723
発行日: 1987/06/30
公開日: 2012/10/29
ジャーナル フリーPDF形式でダウンロード (2220K)
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