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Volume 40 (1998)
- Issue 6 Pages 583-
- Issue 5 Pages 497-
- Issue 4 Pages 213-
- Issue 3 Pages 161-
- Issue 2 Pages 85-
- Issue 1 Pages 1-
Predecessor
Volume 40, Issue 3
Displaying 1-6 of 6 articles from this issue
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Akiko Kukita, Toshio Kukita, Osamu Kohashi1998 Volume 40 Issue 3 Pages 161-168
Published: June 20, 1998
Released on J-STAGE: June 11, 2010
JOURNAL FREE ACCESSSeveral osteoclast differentiation systems have been established with human or rodent bone marrow cells. The effects of several of the factors on osteoclast differentiation have been studied by adding factors to the culture systems. However, these systems include high concentrations of serum in the culture medium, which may influence the effects. We have previously reported that conditioned medium derived from osteoblastic cell line, ROS 17/2.8, (htROSCM), stimulated osteoclast differentiation in rat bone marrow cultures. In this study, we established a system to induce mononuclear preosteoclast-like cells (POCs) by adding serum-free conditioned medium (sf-htROSCM) to the serum-free bone marrow cultures. sf-htROSCM induced the formation of POCs but not multinucleated osteoclast-like cells (MNCs). RT-PCR analysis showed that the cells in this cultures expressed mRNA for calcitonin receptos. In addition, macrophage-colony stimulating factor (M-CSF) promoted the formation of POCs. This system is useful to examine the effects of several factors on the differentiation to POCs in serum-free conditions.View full abstractDownload PDF (5242K) -
Yoshiaki Kamikawa1998 Volume 40 Issue 3 Pages 169-178
Published: June 20, 1998
Released on J-STAGE: June 11, 2010
JOURNAL FREE ACCESSIt is important to determine the extent of invasion to the mandible of carcinoma cells in the treatment of gingival cancer. VX2 carcinoma cells were transplanted into the gingiva in domestic rabbits, and eel calcitonin (ECT) was injected into the thigh muscle. The invasion of VX2 carcinoma cells into the mandible and the effect of ECT were investigated by soft X-ray radiography, light microscopy, and scanning electron microscopy (SEM). In the early phase of bone invasion by VX2 carcinoma, the proliferation of osteoclasts played an important role in the resorption of the bone surface. However, 4 weeks after VX2 carcinoma transplantation, bone resorption was related to the proliferation of the VX2 carcinoma cells, and the number of osteoclasts decreased. SEM observation revealed the difference between the surface of the lacunae resorbed by osteoclasts and the surface of the bone resorption by VX2 carcinoma cells. Soft X-ray radiographs showed that ECT injection inhibited bone resorption by VX2 carcinoma cells. SEM revealed that the ruffled borders of osteoclasts consisted of long finger-like processes and plate-like processes. After ECT injection, the ruffled borders and the surface of the resorption lacunae underwent considerable changes. The ruffled borders of osteoclasts treated with ECT consisted of short plate-like processes.View full abstractDownload PDF (8436K) -
Hideji Komatsu, Takayoshi Tabata, Haruhide Hayashi, Hideaki Mayanagi1998 Volume 40 Issue 3 Pages 179-186
Published: June 20, 1998
Released on J-STAGE: June 11, 2010
JOURNAL FREE ACCESSUnitary discharges of lingual mechanosensitive (LM) neurons after mechanical stimulation of the tongue were recorded from the trigeminal ganglion of the rat. LM neurons were distributed in the caudal part of the mandibular division which is located in the caudolateral part of the trigeminal ganglion. The distribution area of LM neurons overlapped with those neurons sensitive to the oro-facial skin and hair, lip, and mandibular incisor and molar teeth. About 90% of LM neurons were slowly adapting (SA), and the remaining 10% were rapidly adapting (RA). The receptive fields of LM neurons were very narrow (<1mmmm2) and were distributed most densely on the apex, followed by the lateral edge of the tongue. By application of the power law to the relation between the stimulus strength and the magnitude of the responses of SA neurons, the power value to which the stimulus strength was raised was 0.77±0.38. The conduction velocities of LM neurons were less than 2m/sec or 12-16m/sec in the SA neurons, and 12-14m/sec in the RA neurons. These findings suggest that the apex and lateral edge of the tongue are the most important areas in which to discriminate site, intensity and duration of mechanical stimulus, and that lingual sensory input from those regions is important information regarding oro-facial functions, e. g., mastication and deglutition.View full abstractDownload PDF (1721K) -
Yoshikazu Kamino, Mitsunobu Katoh, Eiju Nonaka, Hisashi Takiguchi, Yos ...1998 Volume 40 Issue 3 Pages 187-195
Published: June 20, 1998
Released on J-STAGE: June 11, 2010
JOURNAL FREE ACCESSPorphyromonas, gingivalis, a pathogen in adult periodontitis, produces extracellular vesicles which cannot adhere to the tooth surface. In contrast, Streptococcus gordonii is one of the first oral bacteria to colonize on tooth surfaces and can be expected to support subsequent colonization by other bacteria. We previously reported the cloning of a 40-kDa outer membrane protein (40-kDa OMP) -coding gene from P. gingivalis 381 and that the protein was found to be localized in the vesicles. The aim of the present study was to clarify whether the 40-kDa OMP contributes to the coaggregation of P. gingivalis vesicles with S. gingivalis cells. A flocculation assay revealed that P. gingivalis vesicles and S. gingivalis cells were capable of coaggregation. Heat or protease treatments of P. gingivalis vesicles completely inhibited coaggregation activity, whereas these treatments on S. gingivalis cells only partially reduced coaggregation activity. The coaggregation of this pair was partially inhibited in the presence of an affinity-purified antibody against recombinant 40-kDa OMP or maltose, and was completely inhibited in the presence of both. These findings suggest that there are at least two kinds of adhesin for S. gingivalis in cells on the surface of P. gingivalis vesicles.View full abstractDownload PDF (2970K) -
Yoshiaki Kishi, Masato Matsuo, Toshio Takahashi, Kennichirou Kisara, Y ...1998 Volume 40 Issue 3 Pages 196-200
Published: June 20, 1998
Released on J-STAGE: June 11, 2010
JOURNAL FREE ACCESSDownload PDF (4174K) -
Morimichi Mizuno, Keishi Marumo, Yasuhiko Koyano, Yoshinori Kuboki, Ka ...1998 Volume 40 Issue 3 Pages 201-205
Published: June 20, 1998
Released on J-STAGE: June 11, 2010
JOURNAL FREE ACCESSDownload PDF (4529K)
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