In the current research work, effect of microwave irradiation energy on the esterification of palm fatty acid distillate (PFAD) to produce PFAD methyl ester / biodiesel was intensively appraised. The PFAD is a by-product from refinery of crude palm oil consisting >85% of free fatty acid (FFA). The esterification reaction process with acid catalyst is needed to convert the FFA into fatty acid methyl ester or known as biodiesel. In this work, fabricated microwave-pulse width modulation (MPWM) reactor with controlled temperature was designed to be capable to increase the PFAD biodiesel production rate. The classical optimization technique was used in order to study the relationship and the optimum condition of variables involved. Consequently, by using MPWM reactor, mixture of methanol-to-PFAD molar ratio of 9:1, 1 wt.% of sulfuric acid catalyst, at 55°C reaction temperature within 15 min reaction time gave 99.5% of FFA conversion. The quality assessment and properties of the product were analyzed according to the American Society for Testing and Materials (ASTM), European (EN) standard methods and all results were in agreement with the standard requirements. It revealed that the use of fabricated MPWM with controlled temperature was significantly affecting the rate of esterification reaction and also increased the production yield of PFAD methyl ester.
Coriander (Coriandrum sativum L.) is a major essential oil crop grown throughout the world. Coriander essential oil is extracted from coriander fruits via hydrodistillation, with the industry using 180-240 min of distillation time (DT), but the optimum DT for maximizing essential oil yield, composition of constituents, and antioxidant activities are not known. This research was conducted to determine the effect of DT on coriander oil yield, composition, and bioactivity. The results show that essential oil yield at the shorter DT was low and generally increased with increasing DT with the maximum yields achieved at DT between 40 and 160 min. The concentrations of the low-boiling point essential oil constituents: α-pinene, camphene, β-pinene, myrcene, para-cymene, limonene, and γ-terpinene were higher at shorter DT (< 2.5 min) and decreased with increasing DT; but the trend reversed for the high-boiling point constituents: geraniol and geranyl-acetate. The concentration of the major essential oil constituent, linalool, was 51% at DT 1.15 min, and increased steadily to 68% with increasing DT. In conclusion, 40 min DT is sufficient to maximize yield of essential oil; and different DT can be used to obtain essential oil with differential composition. Its antioxidant capacity was affected by the DT, with 20 and 240 min DT showing higher antioxidant activity. Comparisons of coriander essential oil composition must consider the length of the DT.
A study was conducted to detect and quantify lard stearin (LS) content in canola oil (CaO) using differential scanning calorimetry (DSC). Authentic samples of CaO were obtained from a reliable supplier and the adulterant LS were obtained through a fractional crystallization procedure as reported previously. Pure CaO samples spiked with LS in levels ranging from 5 to 15% (w/w) were analyzed using DSC to obtain their cooling and heating profiles. The results showed that samples contaminated with LS at 5% (w/w) level can be detected using characteristic contaminant peaks appearing in the higher temperature regions (0 to 70°C) of the cooling and heating curves. Pearson correlation analysis of LS content against individual DSC parameters of the adulterant peak namely peak temperature, peak area, peak onset temperature indicated that there were strong correlations between these with the LS content of the CaO admixtures. When these three parameters were engaged as variables in the execution of the stepwise regression procedure, predictive models for determination of LS content in CaO were obtained. The predictive models obtained with single DSC parameter had relatively lower coefficient of determination (R2 value) and higher standard error than the models obtained using two DSC parameters in combination. This study concluded that the predictive models obtained with peak area and peak onset temperature of the adulteration peak would be more accurate for prediction of LS content in CaO based on the highest coefficient of determination (R2 value) and smallest standard error.
The purpose of this study was to investigate three types of extraction methods of extra virgin olive oil (EVOO) from the same cultivar (Ortice olive cultivar): traditional or pressing (T) system, decanter centrifugation (DC) system and a patented horizontal axis decanter centrifugation (HADC) system. Oil samples were subjected to chemical analyses: free acidity, peroxide value, ultraviolet light absorption K232 and K270, total polyphenols, antioxidant capacity, volatile compounds and olfactory characteristics by electronic nose. The two centrifugation systems showed better free acidity and peroxides value but total polyphenol content was particularly high in extra virgin olive oil produced by patented HADC system. Same volatile substances that positively characterize the oil aroma were found in higher amount in the two centrifugation systems, although some differences have been detected between DC and HADC system, other were found in higher amount in extra virgin olive oil produced by T system. The electronic nose analysis confirmed these results, principal component analysis (PCA) and correlation matrix showed the major differences between EVOO produced by T and HADC system. Taken together the results showed that DC and HADC systems produce EVOO with better characteristics than T system and patented HADC is the best extraction system.
A study was carried out to determine the physicochemical characteristics of the oil derived from papaya seeds of the Hong Kong/Sekaki variety. Proximate analysis showed that seeds of the Hong Kong/Sekaki variety contained considerable amount of oil (27.0%). The iodine value, saponification value, unsaponifiable matter and free fatty acid contents of freshly extracted papaya seed oil were 76.9 g I2/100g oil, 193.5 mg KOH/g oil, 1.52% and 0.91%, respectively. The oil had a Lovibond color index of 15.2Y + 5.2B. Papaya seed oil contained ten detectable fatty acids, of which 78.33% were unsaturated. Oleic (73.5%) acid was the dominant fatty acids followed by palmitic acid (15.8%). Based on the high performance liquid chromatography (HPLC) analysis, seven species of triacylglycerols (TAGs) were detected. The predominant TAGs of papaya seed oil were OOO (40.4%), POO (29.1%) and SOO (9.9%) where O, P, and S denote oleic, palmitic and stearic acids, respectively. Thermal analysis by differential scanning calorimetry (DSC) showed that papaya seed oil had its major melting and crystallization transitions at 12.4°C and –48.2°C, respectively. Analysis of the sample by Z-nose (electronic nose) instrument showed that the sample had a high level of volatile compounds.
The effects of antioxidants on the oxidative deterioration of emulsified oils and fats differ depending on the oxidation conditions, oils and fats used, and type of emulsifier. In this study, milk fat was emulsified to obtain water-oil (O/W) emulsion using Tween20 as emulsifier. The antioxidative effects of several antioxidants with various lipophilic properties, such as δ-tocopherol (Toc), epigallocatechin gallate (EGCg), quercetin (Qu), green tea extract (GTE), and rooibos tea extract (RTE) were investigated, the effects of additional emulsifiers such as polyglycerol and sucrose esters of fatty acids on the oxidation stability of the emulsion were also investigated. Under oxidative conditions of 30°C in 650 lx, Toc was more effective than GTE in suppressing the increase of the peroxide value (PV, meq/kg) of the emulsified milk fat. Under these oxidative conditions, the antioxidative effect of GTE was enhanced by the addition of polyglycerol and sucrose esters of fatty acids. Under the oxidative conditions at 40°C in dark with 2,2’-azobis (2-amidinopropane) dihydrochloride (AAPH) or 2,2’-azobis (2,4-dimethylvaleronitrile) (AMVN), Toc showed the most antioxidative effect on suppression of the increase of PV and anisidine value (AnV) of the emulsified milk fat. Furthermore, additional emulsifiers also showed suppressive effects on the increase of the PV and AnV of the emulsified milk fat even without any antioxidants. The effects of additional emulsifiers on the oxidative stability of O/W emulsions were enhanced with antioxidants such as Toc, EGCg, and Qu.
O/W nano-emulsions can be used as effective drug carriers of hydrophobic active ingredients in an aqueous solution, because nano-emulsions are comparatively stable and their structure can be controlled by changing the compositions and the preparation methods. In this paper, we focused on vitamin A and its derivatives (VA), which are among the widely-used lipophilic active ingredients, and tried to develop the nano-emulsions, which can bring out the efficiency of VA for the healing of injured corneas, with the detailed structural analysis of them using the small-angle X-ray scattering (SAXS) method. As a result, we elucidated that the nano-emulsions bearing the hydrophobic oil/water interface can be prepared by decreasing the surfactant concentration against vitamins. Moreover, we clarified that the nano-emulsions composed of lower surfactant concentration tend to adsorb VA onto the corneal epithelial cells-model interface. Therefore it is necessary to prepare the nano-emulsions, which have the hydrophobic oil/water interface for improving the adsorbability onto cell membranes.
In the present study, a purified lipase from Bacillus aerius immobilized on celite matrix was used for synthesis of ethyl ferulate. The celite-bound lipase exposed to glutaraldehyde showed 90.02% binding efficiency. It took two hours to bind maximally onto the support. The pH and temperature optima of the immobilized lipase were same as those of free enzyme i.e 9.5 and 55℃. Among different substrates both free and immobilized lipase showed maximum affinity towards p-nitrophenyl palmitate (p-NPP). The lipase activity was found to be stimulated in the presence of Mg2+ in case of free enzyme while Zn2+ and Fe3+ showed stimulatory effect on immobilized lipase whereas salt ions as well as chelating agents inhibited activity of both free and immobilized lipase. Maximum enzyme activity was observed in n-hexane as organic solvent followed by n-heptane for both free and immobilized lipase, however CCl4, acetone and benzene inhibited the enzyme activity. Moreover, all the selected detergents (SDS, Triton X-100, Tween 80 and Tween 20) had an inhibitory effect on both free and immobilized enzyme activity. The celite bound lipase (1.5%) efficiently performed maximum esterification (2.51 moles/l) of ethanol and ferulic acid (100 mM each, at a molar ratio of 1:3) when incubated at 55℃ for 48 h resulting in the formation of ester ethyl ferulate.
Esterification reaction between different fatty acid namely; lauric, stearic, oleic and linoleic acids and polyethylene glycol-400 were performed. The produced polyethylene glycol ester were reacted with p-amine benzoic acid followed by condensation reaction with germanium dioxide in presence of sodium carbonate to form desired germinate surfactants. The chemical structures of the synthesized surfactants were determined using different spectra tools. The surface parameter including: the critical micelle concentration (CMC), effectiveness (πcmc), efficiency (Pc20), maximum surface excess (Γmax) and minimum surface area (Amin), were calculated from the surface tension measurements. The synthesized surfactants showed higher surface activity. The thermodynamic parameters showed that adsorption and micellization processes are spontaneous. It is clear that the synthesized nonionic surfactants showed their tendency towards adsorption at the interfaces and also micellization in the bulk of their solutions. The synthesized surfactants were tested against different strain of bacteria using inhibition zone diameters. The synthesized surfactants showed good antimicrobial activities against the tested microorganisms including Gram positive, Gram negative as well as fungi. The promising inhibition efficiency of these compounds against the sulfate reducing bacteria facilitates them to be applicable as new categories of sulfate reducing bacteria biocides.
The synthesis of rac 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholines (6a-c), blood platelet activating ether lipid analogues has been achieved in a four-step sequence from epichlorohydrin (1). Etherification of epichlorohydrin with different alcohols namely tetradecyl (2a), hexadecyl (2b) and octadecyl (2c) alcohols gave glycidyl ethers (3a-c) with 78-80% yields. The second step involved opening of the epoxide by acetic anhydride to give acetylated products (4a-c, 76-78% yield), which were subsequently hydrolyzed selectively, a key step of the method employing a 1,3 specific lipase to obtain rac 1-O-alkyl-2- acetylglycerol (5a-c) with 45-50% yields. The hydrolyzed products (5a-c) were phosphorylated to obtain rac 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholines (6a-c) in 80-85% yields.
A commercially purchased acetoacetamide was found to dimerize during storage for several months to afford 4,6-dimethyl-2-pyridone-5-carboxamide. We successfully achieved the quantitative dimerization of acetoacetamide by using an acid catalyst. It was also found that the pyridone formed served as a self-catalyst of the dimerization.
An investigation into the absorption and accumulation of carotenoids from phaffia yeast in two to three-week-old calves was carried out. Carotenoid contents of the control cattle (n=1) were 615.0 ng/g in the liver, 263.7ng/g in the duodenum, 218.0 ng/g in the pancreas, 170.0 ng/g in the blood, 140.3 ng/g in the jejunum, 115.0 ng/g in the spleen. Among the accumulated carotenoids, β-carotene was presented as a major component (86.0 to 94.3%) along with lutein (5.7 to 14.0%) as a minor component. On the other hand, carotenoid contents in phaffia yeast-supplemented (5g/day for one month) calves (n=3) were 4 to 10 times higher than those of the control calf. Carotenoid contents of phaffia yeast-supplemented calves were 2570.1±782 ng/g in the liver, 1806.6±1064 ng/g in the pancreas, 1648.4±630.2 ng/g in the spleen, and 1255.9±300.2 ng/g in the blood. In addition to β-carotene, keto-carotenoids from phaffia yeast, echinenone, (3R)-3-hydroxyechinenone, and (3R,3’R)-astaxanthin, were accumulated in all organs of phaffia yeast-supplemented calves. β-Carotene and (3R)-3-hydroxyechinenone were present as major carotenoids followed by echinenone. However, (3R,3’R)-astaxanthin, which was the major carotenoid in phaffia yeast, was found to be a minor carotenoid in calves. This indicated that calves well absorbed fewer polar xanthophylls, echinenone and (3R)-3-hydroxyechinenone compared to the polar xanthophyll, astaxanthin.
Gluconobacter oxydans IFO12528 is able to produce glyceric acid (GA) from glycerol through the action of a membrane-bound alcohol dehydrogenase (mADH), which is required for GA production. To determine whether membrane-bound aldehyde dehydrogenase (mALDH) also plays a role in GA production in G. oxydans, we constructed an aldH-disrupted mutant of G. oxydans (ΔaldH). ΔaldH was unable to produce acetic acid from ethanol, but was able to produce GA at a level approximately half that of the wildtype strain, suggesting the involvement of another ALDH in GA production. We also investigated the enantiomeric composition of GA produced by the IFO12528 and ΔaldH strains. No difference in GA composition was evident in the ΔaldH mutant, with ~73% d-GA enantiomeric excess observed in both strains.
Important Announcement for the Contributors of the Journal of Oleo Science;
At 0:00 AM on April 1, 2016 (Japan time), the Journal of Oleo Science will start charging Publication Charge for each case; 1: 20,000 yen (within 8 pages. /more than 8 pages, additional charge is necessary; 2,000 yen per page) for Members of Japan Oil Chemists’ Society (JOCS) (including Asian Members and Support Members), 2: 40,000 yen (within 8 pages. /more than 8 pages, additional charge is necessary; 4,000 yen per page) for Non-Members of JOCS.
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