Journal of Oleo Science
Online ISSN : 1347-3352
Print ISSN : 1345-8957
ISSN-L : 1345-8957
Advance online publication
Displaying 1-9 of 9 articles from this issue
  • Yingjun Song, Xu Li, Xiaozhou Liu, Zhaozhong Yu, Guofu Zhang
    Article ID: ess21174
    Published: 2022
    Advance online publication: May 18, 2022
    JOURNAL OPEN ACCESS ADVANCE PUBLICATION

    Spinal injury is a complicated disease and is reported to be associated with damages on spinal astrocytes induced by oxidative injury. Astragali Radi, a famous traditional Chinese medicine, is reported to have promising efficacy in protecting injuries in the central nervous system. This study aims to investigate the effect of calycosin, an isoflavone phytoestrogens isolated from Astragali Radi, on oxidative injury in spinal astrocytes induced by H2O2 and the underlying mechanism. Primary rat spinal astrocytes were pretreated with 5, 10, and 20 μM calycosin and subjected to H2O2 treatment for 24 h to establish an oxidative injury model. Cell viability was detected using the CCK-8 assay to screen the optimized concentration of calycosin. Flow cytometry was used to evaluate the apoptotic rate and cell cycle. The expression level of Brdu was visualized using the immunofluorescence assay. Western blotting was used to measure the expression levels of p-JAK2, p-STAT3, p-AKT, GP130, and IL-6 in spinal astrocytes. We found that proliferation was inhibited and that apoptosis was induced by the stimulation of H2O2. The expression levels of p-JAK2, p-STAT3, p-AKT, GP130, and IL-6 were significantly elevated in H2O2-treated astrocytes. After the treatment of calycosin, proliferation was facilitated, and apoptosis was suppressed. These phenomena were accompanied by the downregulation of p-JAK2, p-STAT3, p-AKT, GP130, and IL-6, which were abolished by the co-administration of PI3K (ly294002) or STAT3 (stattic) inhibitor. Overall, calycosin alleviated oxidative injury in spinal astrocytes by mediating the GP130/JAK/STAT pathway.

    graphical abstract Fullsize Image
    Download PDF (3035K)
  • Daniel Dodoo, Francis Adjei, Samuel Kofi Tulashie, Stephen Awuku, Jack ...
    Article ID: ess21402
    Published: 2022
    Advance online publication: May 18, 2022
    JOURNAL OPEN ACCESS ADVANCE PUBLICATION

    This study conducts postmarketing surveillance for the photosensitised oxidation of vegetable oils (VOs) stored in different conditions in the marketplace during commercialisation. Coconut oil, palm kernel oil, soybean oil and sunflower oil were exposed to direct sunlight and kept in the dark for six weeks. The results showed a significant (p < 0.05) increase in PV and a severe decrease in the iodine value, chlorophyll, β-carotene, colour content, and the fatty acid compositions (oleic and linoleic acids mainly) in the light-exposed VOs. The FTIR analysis also identified the formation of the hydroperoxides (3444 cm–1), secondary oxidation products (1743 – 1723 cm–1) and the loss of the cis-disubstituted olefins (723 cm–1) bands in the light-exposed VOs. This indicated that oils exposed to light for an extended period of time could undergo photosensitised oxidation due to photosensitisers like chlorophyll. In contrast, the unexposed VOs showed no significant change (p > 0.05) in their chemical compositions. The photosensitised oxidation increased in the order: coconut oil < palm kernel oil < soybean oil < sunflower oil.

    graphical abstract Fullsize Image
    Download PDF (5862K)
  • Kento Iwai, Khimiya Wada, Feiyue Hao, Haruyasu Asahara, Nagatoshi Nish ...
    Article ID: ess21406
    Published: 2022
    Advance online publication: May 18, 2022
    JOURNAL OPEN ACCESS ADVANCE PUBLICATION

    Direct aziridination of a nitrostyrene is achieved upon treatment with an alkylamine and N-chlorosuccinimide. The reaction is initiated by the Michael addition of amine to nitroalkene. Subsequent N-chlorination and nucleophilic substitution at the nitrogen atom afford 1-alkyl-2-nitroaziridine diastereoselectively. This reaction mechanism was clarified by NMR studies.

    graphical abstract Fullsize Image
    Download PDF (557K)
  • Takumi Motoyama, Yuka Katsuumi, Hiromu Sasakura, Tetsuya Nakamura, His ...
    Article ID: ess21430
    Published: 2022
    Advance online publication: May 18, 2022
    JOURNAL OPEN ACCESS ADVANCE PUBLICATION

    Oil-in-water (O/W) emulsions containing ethanol have been used in food, cosmetics, paints, and other applications. However, O/W emulsions with long-term stability are difficult to produce at high ethanol concentrations because the adsorption of the emulsifier at the O/W interface is restricted by ethanol. In this study, to resolve this issue, we prepared ethanol-containing O/W emulsions with high dispersion stability using a series of polyglycerol monofatty acid esters (PGFEs) with different fatty acid chain lengths, which are bio-safe nonionic surfactants, as emulsifiers. First, aqueous PGFE solutions containing 0–50 wt% ethanol were prepared and then O/W emulsions were formed using limonene as the oil phase. When decaglycerol stearic acid ester (DGMS, C18) was used as the emulsifier, an O/W emulsion with fine droplets (~30 nm in size) was successfully obtained at an ethanol concentration of 35 wt%. This emulsion remained stable for more than four weeks, during which no phase separation occurred, indicating its high dispersion stability. Furthermore, aqueous DGMS solutions containing 30–40 wt% ethanol were viscous, and a lamellar liquid crystal phase was observed to be dispersed in these solutions. The formation of this lamellar liquid crystal phase at the O/W interface led to an interfacial film with superior viscoelastic properties. The results suggested that the stability of the emulsions was determined by the balance between the decrease in interfacial tension caused by the addition of ethanol and the density (rigidity) of the DGMS film formed at the O/W interface. Finally, to further improve the dispersion stability of the ethanol-containing O/W-type emulsions, O/W emulsions were prepared using a mixture of two PGFEs with different degrees of glycerol polymerization, that is, systems having different hydrophilic–lipophilic balance values.

    graphical abstract Fullsize Image
    Download PDF (2763K)
  • Chunling Huang, Dong Cao
    Article ID: ess22011
    Published: 2022
    Advance online publication: May 18, 2022
    JOURNAL OPEN ACCESS ADVANCE PUBLICATION

    Eggs are nutritious and cheap and easily available. Egg yolk is one of the sources of phosphatidylcholine (PC) and phosphatidylethanolamine (PE). PC and PE have good emulsifying properties, and they are widely used and in high demand for pharmaceutical, feed and cosmetic applications. Red cordyceps egg yolk powder (RCEYP) was selected as the raw material to obtain high content of PC and PE by ethanol extraction and low temperature cryoprecipitation in n-hexane-acetone system (HAS), in which the process conditions of PC and PE extraction by HAS process were optimized. The phospholipids were quantified by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD). The effects of freezing time, material-liquid ratio, acetone washing times, solvent ratio of n-hexane to acetone and freezing temperature on the PC and PE contents and the phospholipid yield were investigated. The optimal conditions for the extraction of PC and PE from RCEYP by HAS were determined by Box-Behnken design (BBD) as follows: the solvent ratio of n-hexane to acetone was 1:6, the freezing time was 11.31 h, and the freezing temperature was –19℃. The total content of (PC+PE) in the phospholipids precipitated under these conditions amounted to 96.16%, of which 81.12% was PC and 15.04% was PE.

    graphical abstract Fullsize Image
    Download PDF (2005K)
  • Qiulian He, Peng Ma, Ramin Torshizi
    Article ID: ess22029
    Published: 2022
    Advance online publication: May 18, 2022
    JOURNAL OPEN ACCESS ADVANCE PUBLICATION

    In this study, some phenolic compounds including 4-Hexylresorcinol, 5-Pentadecylresorcinol, 5-Tricosylresorcinol, Bilobol, and Urushiol were tested against α-glycosidase enzyme from Saccharomyces cerevisiae and sorbitol dehydrogenase enzymes from sheep liver. These compounds determined good inhibition properties against α-glycosidase and sorbitol dehydrogenase (SDH) enzymes. IC50 values were record in the range of 1.45±0.20–24.532±3.83 μM for α-glycosidase and 6.20±0.96–108.22±18.02 μM for SDH. These inhibitor compounds can be selective drug candidates as anti-diabetic agents, because of they have inhibition properties against both enzymes. In this study, the anti-oxidant activities of the molecules were compared with density functional theory (DFT) calculations. Comparison was made with the experimental enzymes by molecular modeling calculations. In the cellular and molecular part of the recent study, the treated cells with some phenolic compounds were assessed by molecularly targeted therapy (MTT) assay for cytotoxicity and anti-acute lymphoblastic leukemia potentials on Clone 15 HL-60, HL-60, HL-60/MX1, and HL-60/MX2 cell lines. The IC50 of these compounds were µg/mL level against these cell lines.

    graphical abstract Fullsize Image
    Download PDF (3058K)
  • Kento Miyamoto, Sakura Hasuike, Hirona Kugo, Wanida Sukketsiri, Tatsuy ...
    Article ID: ess22043
    Published: 2022
    Advance online publication: May 18, 2022
    JOURNAL OPEN ACCESS ADVANCE PUBLICATION

    Women are more resistant to vascular diseases; however, the resistance is reduced after menopause. It has been reported that the risk of vascular diseases such as atherosclerosis and abdominal aortic aneurysm is increased in postmenopausal women. Currently, methods to prevent vascular disease in postmenopausal women have not been established. Isoflavones are promising functional food factors that have a chemical structure similar to estrogen. In this study, we investigated the effects of isoflavones on ovariectomized (OVX)-induced degeneration of the aortic wall in mice. Increased destruction of elastic fibers in the thoracic and abdominal aorta was observed in the OVX group, and isoflavones attenuated the destruction of elastic fibers. The positive areas of matrix metalloproteinase (MMP)-2 and MMP-9 in the OVX group were higher than those in the control group. Isoflavones decreased the positive areas of MMP-2 and MMP-9 compared to those in the OVX group. These data suggest that isoflavones have a suppressive effect on OVX-induced degeneration of the aortic wall by inhibiting the increase in MMP-2 and MMP-9.

    graphical abstract Fullsize Image
    Download PDF (1600K)
  • Minoru Yoshimoto, Shigeru Kurosawa, Mutsuo Tanaka
    Article ID: ess22049
    Published: 2022
    Advance online publication: May 18, 2022
    JOURNAL OPEN ACCESS ADVANCE PUBLICATION

    The temperature dependence of the resonant length, molecular weight, and rheology (shear viscosity and shear modulus) of chemisorbed soft matter on a solid-liquid interface oscillating at a megahertz frequency was studied using a quartz crystal microbalance. As a form of chemisorbed soft matter, self-assembled monolayers (SAMs) formed from six types of mercapto oligo(ethylene oxide) methyl ethers were used. A systematic analysis using the Voigt model showed that the variation in effective hydrated thickness (sensed mass), which is related to the resonant length, was classified into three types based on the molecular weight. As a result, a 2.2-nm change in the resonant length occurred in the studied temperature range from 10 to 35℃. Moreover, the variation in the effective hydrated thickness was dependent on the shear viscosity and shear modulus of the SAMs. A further investigation revealed that the relationships η1Mn0.13 and μ1Mn0.30 could be estimated regardless of the temperature, where η1 and μ1 are the shear viscosity and shear modulus of the SAM, and Mn is the molecular weight of mercapto oligo(ethylene oxide) methyl ether. As a result, we revealed that the experimental results followed the polymer formula irrespective of temperature.

    graphical abstract Fullsize Image
    Download PDF (596K)
  • Masato Amano, Kazuaki Hashimoto, Hirobumi Shibata
    Article ID: ess22061
    Published: 2022
    Advance online publication: May 18, 2022
    JOURNAL OPEN ACCESS ADVANCE PUBLICATION

    Hexagonal plate-like ZnO particles with a high degree of c-face orientation have been synthesized using hydrothermal method in the presence of various anionic surfactants bearing different hydrocarbon chains. The c-face of the ZnO particles increased upon increasing the surfactant alkyl chain length. The photocatalytic activity of the as-obtained hexagonal plate-like ZnO particles was evaluated using the degradation of methylene blue (MB). Although the specific surface area of hexagonal rod-like particles is higher than that of hexagonal plate-like particles, the amount of MB adsorption on the ZnO particle surface was different for the hexagonal plate-like and rod-like particles. In addition, the hexagonal plate-like ZnO particles showed a significantly higher decrease in the MB concentration with the duration of ultraviolet light irradiation when compared to the hexagonal rod-like ZnO particles obtained in the absence of a surfactant. These results indicate that crystal-face-controlled ZnO with a high degree of c-face orientation exhibits high photocatalytic activity.

    graphical abstract Fullsize Image
    Download PDF (809K)
feedback
Top