Fifty traditional Tibetan ghee (TTG) varieties were collected and analyzed their systematic characteristic indices, including physicochemical parameters, minerals, fatty acid composition, and thermal behavior. Results show that TTG contains a large amount of fat (71.68%–93.3%) and a small quantity of protein (0.51%–1.81%). The acid and peroxide values of TTG vary from 0.02 to 1.30 mg/g and 0.07 to 5.93 meq/kg, respectively. The content of minerals varied with altitude level and region significantly (p < 0.05), and the regional variations of fatty acids in TTG were also observed significantly, these differences may be due to the high unsaturated fatty acids level in the cow diets.
Enzyme-assisted aqueous extraction of rice germ oil (RGO) was performed in this study. The physicochemical properties, fatty acid composition, bioactive substances and antioxidant activity of RGO were analyzed. An enzyme composed of alcalase and cellulase (1:1, w/w) was found to be the most effective in the extraction yield of oil. The optimal oil yield of 22.27% was achieved under the conditions of an enzyme concentration of 2% (w/w), incubation time of 5 h, incubation temperature of 50°C, water to seed ratio of 5:1, and pH 6.0. The predominant fatty acids of RGO were oleic acid (39.60%), linoleic acid (34.20%) and palmitic acid (20.10%). The total saturated fatty acid (SFA), monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) composition of RGO were 22.50%, 39.60% and 36.00%, respectively. RGO yielded a high content of γ-oryzanol (530 mg/100 g oil), tocotrienol (62.96 mg/100 g oil), tocopherol (23.24 mg/100 g oil) and a significant amount of phytosterol (372.14 mg/100 g oil). It exhibited notable antioxidant activities with IC50 values of 32.37 and 41.13 mg/mL, according to the DPPH radical scavenging assay and β-carotene/linoleic acid bleaching test, respectively.
Elaeagnus mollis oil (EMO), which is a type of plant oil, was extracted from the nuts of Elaeagnus mollis Diels that is known as a precious woodyoilcrop in China. The present study investigated the ameliorative effects of EMO on high-fat diet-induced non-alcoholic fatty liver disease (NAFLD) and explored relative regulation mechanism. The analysis of EMO fatty acids showed that EMO rich in unsaturated fatty acids (92.07%), such as linoleic acid (48.24%), oleic acid (34.20%) and linolenic acid (7.57%). In addition, supplementation of EMO could ameliorate the increase in body weight, fat weight, and abnormal serum lipids induced by high-fat diet. A further important implication is that the levels of serum ALT, serum AST, hepatic TG, TC, SOD, GSH/GSSG ration and MDA were improved after supplementing with EMO. All these changes may be due to the ability of EMO to inhibit fatty acid synthesis via reducing the mRNA expression of SREBP-1c, PPARγ and FAS, and elevate fatty acid oxidation by increasing the mRNA expression of PPARα and CPT-1. Meanwhile, our results also showed that endogenously synthesized n-3 PUFAs could significantly increase after treating with EMO. In conclusion, the results suggested that EMO could be regarded as a healthy food for preventing NAFLD.
The essential oil extracted from roots and rhizomes of Ligusticum jeholense Nakai et Kitagawa was investigated for its chemical composition by GC-MS analysis, and evaluated for its contact toxicity and repellency against Tribolium castaneum and Lasioderma serricorne, along with some of its individual components. The essential oil was rich in aromatics (65.34%) with low molecular weight. Major components included sedanolide (33.95%), 3-butylidenephthalide (18.76%), spathulenol (8.90%) and myristicin (6.76%). The results of bioassays indicated that the essential oil of L. jeholense and 3-butylidenephthalide possessed significant repellent activities against T. castaneum at 2 and 4 h post-exposure. Meanwhile, 3-butylidenephthalide had potent contact toxicity against L. serricorne (LD50 = 13.64 µg/adult). The minor component n-butylbenzene in the oil was highly toxic to T. castaneum (LD50 = 23.99 µg/adult) and L. serricorne (LD50 = 7.86 µg/adult) in contact assays, but failed to repel these beetles at all testing concentrations. Spathulenol and myristicin exerted good insecticidal and repellent effects on the two target insects. This work suggests that the essential oil of L. jeholense has promising potential for development as natural insecticide or repellent to control pest damage in warehouses.
We investigated the solubilization behavior of the hydrocarbon surfactant lithium dodecyl sulfate (LiDS) and the fluorocarbon surfactant lithium 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-1-octanesulfonate (LiFOS) in an aqueous solution to determine the controlled release mechanism of solubilizate. The LiDS system solubilized Sudan III, a hydrocarbon compound, whereas the LiFOS system did not, because of the immiscibility of the hydrocarbon and fluorocarbon compounds. The solubilization ability of the LiDS and LiFOS mixtures gradually decreased with increasing LiFOS bulk composition because the micelles mainly composed of LiDS transformed into micelles mainly composed of LiFOS. Furthermore, Sudan III solubilized in the aqueous LiDS was deposited when an aqueous LiFOS was added. The difference in the solubilization behavior between LiDS and LiFOS enabled the controlled release of the solubilizate.
Gels exhibit complex friction behavior. This study aims to evaluate the friction forces between two fractal agar gel substrates under sinusoidal motion to show the effect of rough surfaces on friction dynamics. In a previous study, we observed an asymmetric friction profile during reciprocating motion and an ultra-low friction state on flat agar gel surfaces. On the other hand, these distinct friction profiles were not observed on rough agar gel surfaces. We determined that this distinction was caused by the contact state between fractal agar gel surfaces; no thick water film was formed on the fractal surfaces because the rough structure provided channels to drain water from the interface. These physical insights are useful not only for developing biofunctional materials but also for understanding surface phenomena on biosurfaces including tongues and small intestinal walls.
Producing structural viscosity in colloidal dispersions, such as vesicles and capsules, prevents separation of dispersed particles by increasing the viscosity between them, which is advantageous in terms of usability. So far, the separation behavior of various particles has been studied; however, there are very few examples wherein a stable dispersion state was constructed and controlled. In this study, we produced stable dispersions induced by the depletion effect in mixtures of vesicles of cationic surfactant derived from triethanolamine-based esterquat (TEQ) and a specific dextrin derivative (SDD) as a non-adsorptive polymer. In the composition region, where 8 to 16% of TEQ vesicles and 1.2% or less of SDDs were mixed, the viscosity increased proportionally with the particle concentration, and it was observed that stable dispersions were produced by structural viscosity. Furthermore, the effects of TEQ and SDD concentrations, and SDD size on the structural viscosity and cohesive energy were investigated, which were similar to the depletion effect in the Asakura–Oosawa (AO) theory. From the results, it was suggested that the structural viscosity of the mixed dispersions (TEQ vesicles and SDDs) was produced by the aggregated TEQ vesicle networks induced by the depletion flocculation.
Cardiovascular disease (CVD) has emerged as the leading cause of dealth worldwide today. Lowering circulating total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) is one of the most effective approaches of CVD prevention. Dietary guidelines and health organizations approved using plant sterols (PS) as the alternative to conventional method in attenuating circulating TC and LDL-C levels and risk of CVD. However, current findings apprear to be controversial on the efficacy of PS. Giving the rise of the field “Nutrigenetics”, single nucleotide polymorphisms (SNPs) such as CYP7A1-rs3808607 have been identified that strongly associate with cholesterol metabolism in response to PS intake, towards causing inter-individual variations. This review article aims to discuss the efficacy of dietary PS in managing cholesterol levels based on findings from recent studies. The scope includes reviewing evidence on supporting the efficacy, the metabolic claims, inter-individual variations as well as sitosterolemia associated with PS intake.
In this study virgin olive oil obtained from cv. Nizip Yaglik (NY) was adulterated with different proportions (5 and 10%, v/v) of cotton (CO) and sunflower (SO) oils. Fatty acid and sterol profiles of olive oil were analyzed by using gas chromatography (GC). Also, difference of Equivalent Carbon Number 42 values (ΔECN42) of oil samples were determined by using GC and HPLC. Due to results of fatty acids analysis, the percentage of oleic acid was decreased when CO and SO were added. Palmitic acid was increased over the addition of CO, and decreased with the addition of SO. The ΔECN42 values were increased in adulterated oils. These values showed further increase in adulterated oils with SO. Beta-sitosterols decreased to 91.06 and 88.54% when mixed with 5 and 10% SO, respectively. On the other hand, decline was negligible when mixed with CO. According to principal component analyses (PCA), pure NY and adulterated oils were clearly separated in different parts of screen plot according to fatty acids, triacylglycerol (TAGs) and sterol profile. The outcomes of this first investigation provide valuable information for about the differences of fatty acids, ΔECN42 values and sterol compounds between Turkish olive oil from Nizip Yaglik cv. and its adulteration with cotton and sunflower oil. It was observed that fatty acids are not very effective in detecting adulteration of NY oil, but ΔECN values, sterols and Rmar values can be used to detect adulteration of NY olive oil.
Ginkgo biloba extract (GBE) is widely used as herbal medicine. Preventive effect of GBE against dementia, including Alzheimer’s disease, has been reported. The bioactive compounds in GBE that impart these beneficial effects, flavonoids and terpene lactones, have poor bioavailability. Our previous study found distribution of bioactive compounds of sesame extract in mice brain after mixing it with turmeric oil. Here, we evaluate the distribution of bioactive compounds of GBE by combining it with the mixture of sesame extract and turmeric oil (MST). The content of terpene lactones in mice serum was significantly increased in a dose-dependent manner after administration of GBE. However, the contents of terpene lactones in mice brain were not significantly changed. Concentration of ginkgolide A in mice brain increased significantly when GBE was co-administrated with MST than when GBE was administered alone. These results suggest that MST may be effective in enhancing the bioavailability of ginkgolide A in GBE.
This work reports the chemical composition, antimicrobial and antioxidant activities of essential oils from the aerial parts of Gentiana gelida BIEB. for the first time in literature. The oils from the aerial parts (flower, leaf and stem) were obtained by Clevenger-type apparatus and characterized by GC-FID and GC-MS. While tricosane (21.67%) was the main component of flower oil, hexadecanoic acid was the most abundant component of leaf and stem oils in ratios 26.46% and 31.89%, respectively. Additionally, all essential oils of G. gelida were investigated for their antimicrobial activity against eight Gram negative bacteria, four Gram positive bacteria and five fungi, using agar dilution method and antioxidant activities by using 2,2-diphenyl-1-picrylhydrazyl radical scavenging and Folin-Ciocalteu assays. The flower oil of G. gelida showed stronger antimicrobial and antioxidant activities than those of stem and leaf. The amount of total phenolic content and scavenging activity of the flower oil were found 525.35±8.24 mg GAE/L and 49.30±1.25%, respectively.