Human papillomavirus (HPV) has been known as a pathogen of oral dysplasia. However, suitable PCR primers for the detection of oral HPV infection have not been reported. The aim of this study was to design unique consensus primers. The consensus primers were designed by homologues analyses between subtypes 2, 6, 11, 13, 16, 18, 30, 32 and 58, which frequently infect the oral membrane. PCR, with our designed primer, detected HPV DNA subtypes 2, 6, 11, 16, 18 and 58, and also showed PCR product from a clinical papilloma sample. These results indicate that our designed consensus primer can be used for the study of the relationships between oral disease and HPV infection. (J. Oral Sci. 42, 189-193, 2000)
The aim of the current study was to evaluate the effects of functional treatment appliances, a U-Bugel type I activator and a conventional activator, on the mandibular condyle considering the sagittal direction. Alterations in the growth direction and quantity of the condyle effects the ramal inclination and, consequently, the whole mandible. This situation is important when considering treatment prognosis and stability. The material consisted of pre-and posttreatment lateral cephalograms and hand-wrist films of 49 individuals having skeletal and dental Class II/div.1 malocclusions. Although the elimination of overjet and correction of Class II/div.1 anomalies was achieved, statistically significant changes were not observed in the sagittal direction of the mandibular condyle. (J. Oral Sci. 42, 195-203, 2000)
The turnaround time (TAT) for oral biopsies received for histological examination by the Department of Oral Pathology, Oral Medicine and Periodontology, Faculty of Dentistry, University of Malaya, for the years 1978, 1988 and 1998 was evaluated. For the three years studied, TATs for 61, 233 and 463 specimens were retrospectively analysed. Testing intervals, that is, from the dates the surgeons procured the specimens, the laboratories accessioned them and until the pathologists signed off the diagnoses, were used to calculate TAT. The performance level of the respective pathologists, the growth of tissue diagnostic services and the possible variables that influence TAT were also evaluated. As prompt diagnosis means prompt treatment, which in turn has a bearing on prognosis, the TAT pertinent to oral malignant tumors was emphasized. The mean TAT, its mode and median fell significantly in 1998 compared with the previous 2 years; it was lower for soft tissue than for hard tissue specimens, and lower for malignant, than for non-malignant specimens. The progression of tissue diagnostic services is up to a satisfactory level, as 88.89 % of biopsies could render diagnoses within a fair period of time in 1998. (J. Oral Sci. 42, 205-210, 2000)
The purpose of this study was to evaluate the efficacy of three-dimensional (3-D) images produced with a helical CT for the diagnosis of lesions occurring in the maxillofacial region. Thirty-four patients, who had lesions in the maxillofacial region, were examined by plain radiography (intra and extraoral) and the helical CT. Further, 3-D images were reconstructed from the data provided by the helical CT using the volume rendering method. These images were compared with plain radiographic images and conventional two-dimensional (2-D) CT images in terms of the information they provided for diagnosis. Using the 3-D images for tumors, bone destruction, inner components, extent of the lesion, the relationship between the lesion and surrounding anatomical landmarks, and the roots of the adjacent teeth were observed in overall views. We conclude that 3-D images produced by helical CT may provide useful information for the diagnosis of lesions. (J. Oral Sci. 42, 211-219, 2000)
The accumulation of mitochondrial DNA mutation is an important contributor to the aging process. Polymerase chain reaction (PCR) amplification was carried out on total DNA from gingival tissues of human subjects with ages of 19 to 64 years, and 5.0-kb and 7.4-kb deletions were found in the mitochondrial genomes of these subjects. This is the first report of mitochondrial DNA deletion detectable in human gingival tissues. (J. Oral Sci. 42, 221-223, 2000)
Helicobacter pylori is an important pathogen involved in the development of gastrointestinal ulcers, but its involvement in oral ulcerous lesions is unclear. As culture is generally recognized as the gold standard for diagnosis of H. pylori infection, we employed this approach to assess the association of H. pylori with oral mucosal ulcerations. Samples were collected from patients with oral mucosal ulcerative disorders : 12 cases of recurrent aphthous stomatitis (RAS), 7 cases of herpes simplex virus (HSV) stomatitis, and 3 cases of erosive lichen planus (LP). Serum IgG antibodies against H. pylori were examined in all cases. All of the RAS and erosive LP cases were culturenegative for H. pylori, while two cases of HSV stomatitis were positive. The two culture-positive cases were also seropositve for the H. pylori antigen. It is suggested that H. pylori might not have a direct association with oral ulcerations. However, H. pylori in the oral cavity might exist in a non-culturable coccoid state without productive infection, and might form colonies only under special conditions such as HSV infection. (J. Oral Sci. 42, 225-2293, 2000)
In order to analyze the possible relationship between the quantity of gingival crevicular fluid (GCF) and clinical periodontal status, the severity of gingival inflammation (gingival index (GI) scores) and probing depth (PD) were recorded and GCF samples were obtained from 1111 sites. These sites were further analyzed on the basis of distinct tooth groups to evaluate the significance of particular anatomical sampling locations. Statistical analysis of cumulative data showed significant increases in GCF volume with greater GI scores and PD. Correlations between GCF volume and both of the clinical measures were also strongly positive and significant for all sites. However, significant differences in GCF volume were observed between the anterior and posterior sampling sites. Increases in volume with increasing GI and PD were more marked for incisor and canine teeth. Similarly, the relationship between the quantity of GCF and clinical periodontal status was more clear and absolute in the anterior region than in the premolar and molar areas. These findings suggest that the quantity of GCF is not constant throughout the entire dentition, and that the relationship between GCF measurements and clinical periodontal status is site-based. This unique feature of GCF seems to be an essential factor in the design of GCF-related studies. (J. Oral Sci. 42, 231-238, 2000)
Levels of messenger RNA (mRNA) extracted from five samples of radicular cyst-lining epithelium were analyzed for cytokines, growth factors and epithelial cell growth-related receptors by RT-PCR. All five samples expressed IL-1α, -1β, IL-6, IL-8, IL-11, TGF-β1, PDGF-A and aFGF, and receptors for EGF (c-erbB), KGF, HGF (c-met) and IL-6. Some of the specimens expressed MIP-1α, RANTES, GM-CSF, M-CSF, TNF-α, PDGF-B and bFGF, but no expression of IL-2, IL-4, IFN-γ, IGF-I, EGF and KGF was detected. These results indicate that radicular cyst-lining epithelium, which is considered to be identical to the cell rests of Malassez, may play a role in periodontal pocket formation or apical cyst formation by interaction with surrounding connective tissue or hematopoietic cells through the expression of various cytokines. (J. Oral Sci. 42, 239-246, 2000)
Myiasis is the invasion of living tissue of humans and other mammals by the eggs or larvae of flies of the order of Diptera. It occurs mainly in the tropic, and is associated with inadequate public and personal hygiene. Oral myiasis in humans appears to be rare. This article records a case of oral myiasis caused by larvae of Hypoderma bovis. Two different pathologic soft tissue sockets were observed in the vestibular sulcus at the level of the both deciduous laterals along the deep upper lip tissues. (J. Oral Sci. 42, 247-249, 2000)
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