Toxic doses of cholecalciferol (vitamin D3) were injected subcutaneously (25μg/day) into rats for 5 weeks, causing a significant increase of serum alkaline phosphatase (ALP) levels (p < 0.01). Gingival tissue alkaline phosphatase levels were also elevated (p < 0.01). On the other hand, a significant difference was detected between serum calcium levels in experimental and control groups (p < 0.02).
A cytochemical study was performed on the lamprey ventral aorta with special reference to disulfide and sulfhydryl groups of microfibrils, using the high-iron diamine-thiocarbohydrazide-silver proteinate (HID-TCH-SP) method combined with several other types of treatment. The HID-TCH-SP staining observed was classified into three categories : 1) weak staining in the periphery of collagen fibrils, 2) moderate staining in the boundaries of collagen fibrils, microfibrils and smooth muscle cells, and 3) intense staining of microfibrils. The first and second categories of staining were considered to represent chondroitin and/or heparan sulfate because of sensitivity of the staining to chondroitinase ABC (ChABC) and its specific localization. By contrast, the third category of staining was considered to represent disulfide and sulfhydryl groups of microfibrillar glycoprotein, because it was disclosed only after Oxone oxidation or thiosulfation and was not removed by ChABC digestion. Although this staining reactivity was not apparently altered by SH blockade prior to oxidation or thiosulfation, it was markedly diminished or completely inhibited by S-S reduction followed by SH blockade. These results indicate that lamprey aortic microfibrils contain more S-S groups than SH groups.
A case of undifferentiated carcinoma arising in the minor salivary gland is reported. Preoperative chemotherapy, surgery and postoperative immunotherapy were performed and good results were obtained for 15 months after surgery. However, metastasis to the lung occurred 18 months after surgery. The histologic diagnosis, clinical findings and origin of this tumor are discussed.
X-ray powder diffraction of red kangaroo (Macropus rufus) dental emanel, with a relative density greater than2.60, showed a longer a-axis length of the apatite unit cell, a = 9.462 Å, a shorter c-axis length, c= 6.877 Å, and a smaller crystallite size, ca. 185 Å, than those of human dental enamel. The a-axis of red kangaroo dental enamel was one of the longest among the values reported previously, whereas the c-axis was one of the shortest. The crystallite size was almost equal to that of dentin crystallites. It is suggested that the chemical composition of the dental enamel apatite of the red kangaroo is markedly substituted, resulting in lattice distortion
The influence of different non-resorbable suture materials on the healing of tooth extraction wounds was examined in 120 adult male albino rats. Nylon gave the best biological results for healing of the mucosa and dental socket. Silk and cotton produced considerable delay of socket healing. Healing was only mildly delayed by polyester suture, with a short moderate inflammatory reaction at the initial stage.
Seventy-two male albino rats received autogenous transplants of glycerol-preserved rib cartilage into the malar process. The animals were divided into two groups which received preserved cartilage with or without perichondrium. The implants were well tolerated and removal of the perichondrium enhanced the rate of resorption and bone replacement of the material.