Levels of serum IgG against Porphyromonas gingivalis cell sonicate were determined in patients with rheumatoid arthritis (RA) (n=25), rapidly progressive periodontitis (RPP) (n=25) and adult periodontitis (AP) (n=15) and controls (HP) (n=10) utilizing the ELISA technique. Comparison between groups showed no significant differences between the HP and RA groups and also between the RPP and AP groups. The increased levels of IgG in the RPP and AP groups were comparable. Significant differences in IgG levels were noted between HP and RPP (p<0.05) and between HP and AP (p<0.01). The differences between RA and RPP and between RA and AP were highly significant (p<0.0001). Thus it was revealed that the serum levels of IgG against P. gingivalis in RPP and AP patients were elevated, whereas the levels in RA patients were comparable to those in controls.
An epithelial Madin-Darby canine kidney (MDCK) cell line was grown in two kinds of standard media and a medium conditioned by MRC-5 embryonic lung cells, and temporo-regional differences in cell proliferation were examined by immunohistochemical detection of incorporated bromodeoxyuridine (BrdU). MRC-5-conditioned medium is known to contain scatter factor, which induces scattering of MDCK cells. In a proliferation assay, the BrdU labeling index (percentage ratio of BrdU-positive to total nuclei) was highest in day 2 cultures in the standard media, but on day 3 in the conditioned medium. MDCK cells in the standard media formed multiple epithelial sheets by day 2. In the conditioned medium, however, epithelial sheet formation was retarded and observed only after day 3. Once epithelial sheets had formed, cells close to the edge of the sheet were more proliferative than those distant from the edge in both standard and conditioned media. These findings suggest that proliferation of MDCK cells is suppressed during the scattering induced by the conditioned medium, and that their DNA synthesis becomes most active when cell scattering has ceased and epithelial sheets have begun to form.
Doppler auscultation as applied to internal derangements of the TMJ has been developed in recent years. Possible clinical use of TMJ Doppler auscultation was examined by separating the signals into their spectral components, and comparing the results with those of the conventional sound amplification system (CSAS). Observing frequency component changes with time, similar observations were made using CSAS and TMJ Doppler. However, noises found in TMJ Doppler sonograms were markedly reduced in comparison with those found using CSAS. Ultrasonic waves have high directivity, and pass through soft tissue and fluid; however they have difficulty passing through air, and this may decrease the recording noise. TMJ Doppler can be applied to record movements of the condyle because changes in the receiving frequency indicate the movement of an observed object with precision.
Ehlers-Danlos syndrome (EDS), a group of rare, autosomal dominantly inherited connective tissue dysplasias, characterized mainly by abnormal collagen synthesis, has been shown to exhibit extensive heterogeneity with at least 11 clinical entities differentiated by their clinical, biochemical, and genetic features. Of these, Type VIII EDS is of special interest from a dental viewpoint, due mainly to the resulting fragility of the oral mucosa and blood vessels, and an aggressive type of periodontitis causing severe loss of alveolar bone; all of these clinical manifestations of the entity make it necessary to exercise care during the den-tal treatment procedures. Here we present a case of EDS Type VIII with typical clinical findings, together with details of the dental treatment modalities, placing emphasis on surgical methods.
Four cases of malignant lymphomas in elderly patients are reported, along with the results of immunohistochemistry and electron microscopy. Light microscopy revealed that three cases consisted of diffuse neoplastic proliferation of mediumsized lymphocytes (diffuse lymphoma, medium-sized cell type), while the remaining one showed follicular neoplastic proliferation of medium-sized lymphocytes (follicular lymphoma, medium-sized cell type). Immunohistochemical analysis revealed that most of the tumor cells in three cases had weak to moderate immunoreactivity for L26 and weak reactivity for IgM. Most of the tumor cells in one case showed weak reactivity for IgG. Some tumor cells in three cases had weak reactivity for vimentin, and some in two cases showed weak positivity for KP1 and PG-M 1. Ultrastructural examination revealed that tumor cells in all four cases contained large nuclei with clear nucleoli and scant cytoplasm. Most of the intercellular junctions were smooth, but in one case, some areas exhibited desmosomes.