The influence conjugated linoleic acid (CLA) or linoleic acid (LA) has on the skin leukotriene B₄ (LTB₄) content of pregnant mothers and the offspring was investigated in mice. The female mice (8-week-old) were separated into three groups and given either a commercial diet, a diet containing 1% LA, or a diet containing 1% CLA. The skin from the mothers and their offspring at 0,1 and 2 weeks after birth was analyzed for LTB₄ content. No significant difference in LTB4content was observed in mother mice or their offspring at 0 and 1 week of age. However, the LTB4content in the LA group at 2 weeks of age was significantly higher than the control group, while not significant in the CLA group. In conclusion, orally administered LA, but not CLA, enhanced the skin LTB₄ content of murine offspring.

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We added α-tocopherol to 5 different commercial dog foods after their bags were opened to see how their fat changes in terms of acid value, peroxide value, and iodine value for 30 days. The total amount of Vitamin E was also measured. The fat contents of the dog foods were 6% (in 2 of them),10%,15% and 18%.
1. The acid value of the 5 dog foods ranged from 4.7 mg/g and 7.3 mg/g immediately after their bags were opened. These values were not toxic to dogs. The acid values in those dog foods changed little with time, so α-tocopherol did not show any antioxidant effect. The acid values of the dog foods during storage were within the safe range for dogs.
2. Judging by the changes in the value of peroxide value with time, the amounts of α-tocopherol that we used in our experiment did not seem to be enough to keep the value of peroxide value stable.
3. The iodine values of all the 5 dog foods used in this experiment tended to decline with time, and their values were less than 90. So, we judged that the fat in the dog foods is a nondrying oil. The addition of α-tocopherol resulted in little change in the iodine value.
4. For the analysis of Vitamin E with HPLC, we tried to cut the extraction time and save the amount of reagent by reducing the number of washing when washing the water layer with petroleum ether after the saponification of the reagent. We found that the extract after two washing still contained Vitamin E which is about 10% of the amount found in the extract after the first washing. So we chose to repeat the washing process twice for our analysis. In the total amount of Vitamin E, α-tocopherol was 97% of the total in the control and 99% in the dog foods with addedatocopherol. The amounts of β-tocopherol and γ-tocopherol were 0.8-1.9%. The amount of α-tocopherol in the dog foods tended to decline with time due to fat rancidity. The detected amounts of β-and Y-tocopherol were small, indicating that there was no anti-oxidant effect of α-tocopherol.

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Urinary constituents of apparently healthy adult cats were compared with those of adult dogs. Urinary pH tended to be higher in adult dogs, although it was not statistically significant. In contrast, urine osmolality and urinary concentration of total protein were significantly higher in adult cats. Urinary concentrations of Mg and NH₃ were higher in adult cats, whereas urinary concentration of P was higher in adult dogs. SDS-PAGE of urinary sediment of adult cats exhibited a band resembling THP. The THP-like protein, however, could not be detected in urinary sediment of adult dogs. There was no striking difference on a pattern of urinary nitrogenous compounds between adult cats and adult dogs, although the percentage of urinary urea-nitrogen to urinary total nitrogen tended to be higher in adult cats. These results suggest differences of urinary constituents between clinically normal adult cats and adult dogs.

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