Giant clam Calyptogena soyoae colonies were discovered on the deep seafloor of Sagami Bay. The biological activity of the colony is considered to be kept by sulfur and carbon compounds in upwelling fluid at the site. The radioisotope ratio
14C/
12C and stable isotope ratio
13C/
12C in the giant clam shells were measured to confirm the fluid upwelling and to investigate the origin of the fluid.
The ratio
14C/
12C was measured on specimens collected from two areas: Off-Hatsushima and the Sagami Knoll. The values were scattered over a wide range: normalized isotope ratio in per mil, δ
14C= -347 to -159%o. Even the living clams have various δ
14C values, and have not the highest δ
14C in the colony. These data indicate that the δ
14C value does not correspond to the age of the shell, but to the degree of contamination of the seawater around the shell with the upwelling fluid.
The ratios
13C/
12C and
14C/
12C were measured on specimens collected from the Sagami Knoll. The data showed linear relation between the values of
14C/
12C and
13C/
12C. We infer from the linear relation that the carbon in the shells came from two sources, ordinary bottom seawater (Δ
14C = -220‰, δ
13C= 0‰) and upwelling fluid (Δ
14C = -1, 000‰, δ
13C= -33‰). The δ
13C value suggests that the carbon in the upwelling fluid has its origin in sedimentary rocks. In addition, the Δ
14C value suggests that the sedimentary rocks are older than several tens of thousands of years.
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