Journal of Pesticide Science
Online ISSN : 1349-0923
Print ISSN : 1348-589X
ISSN-L : 0385-1559
35 巻, 4 号
選択された号の論文の39件中1~39を表示しています
Original Articles
  • Kenjiro Furuta, Norihiro Fujita, Tsubasa Ibushi, Takahiro Shiotsuki, N ...
    原稿種別: Original Article
    2010 年 35 巻 4 号 p. 405-411
    発行日: 2010/11/25
    公開日: 2010/11/24
    [早期公開] 公開日: 2010/06/18
    ジャーナル フリー
    Ethyl 4-[(6-substituted 2,2-dimethyl-2H-chromen-7-yl)methoxy]benzoates and their analogues were prepared and the biological activities were evaluated for both anti-juvenile hormone (anti-JH) and JH activity in silkworm larvae, Bombyx mori. Of the compounds tested, ethyl 4-[(6-methoxy-2,2-dimethyl-2H-chromen-7-yl)methoxy]benzoate (3b) showed the most effective precocious metamorphosis-inducing activity in 3rd instar larvae and JH activity in allatectomized 4th instar larvae. Furthermore, JH I and 20-hydroxyecdysone (20-E) titers in hemolymph of 3rd instar larvae treated with 3b were measured by liquid chromatography-mass spectrometry (LC-MS) and LC-MS/MS, respectively. The results revealed that compound 3b induced precocious metamorphosis by specifically decreasing JH I in hemolymph.
  • Kwang-Wook Ko, Kazunori Okada, Jinichiro Koga, Yusuke Jikumaru, Hideak ...
    原稿種別: Original Article
    2010 年 35 巻 4 号 p. 412-418
    発行日: 2010/11/25
    公開日: 2010/11/24
    [早期公開] 公開日: 2010/06/18
    ジャーナル フリー
    The production of phytoalexins is a well-documented defense response against pathogen infection in rice. Although jasmonic acid has been suggested to be involved in the production of phytoalexins in both suspension-cultured rice cells and rice leaves, there has been almost no information on other secondary signaling molecules that regulate the production of phytoalexins in rice. In this study, the production of the major diterpenoid phytoalexins, momilactones and phytocassanes, was found to be induced by cytokinin treatment in both suspension-cultured rice cells and rice leaves, and also the upregulation of phytoalexin biosynthetic genes was found to be induced by cytokinin treatment. The roles of cytokinins in the production of diterpenoid phytoalexins in rice infected with a pathogen are discussed.
  • Hideaki Tateishi, Taiji Miyake, Masaru Mori, Rie Kimura, Yoneko Sakuma ...
    原稿種別: Original Article
    2010 年 35 巻 4 号 p. 419-430
    発行日: 2010/11/25
    公開日: 2010/11/24
    [早期公開] 公開日: 2010/07/02
    ジャーナル フリー
    The sensitivity of the Fusarium graminearum species complex, the causal fungus of Fusarium head blight, to metconazole was measured. The minimum inhibitory concentrations (MIC) of 101 isolates ranged from 0.20 to 6.25 mg/l with a single peak at 1.56 mg/l. The effective concentration for 50% growth inhibition (EC50) was <0.1 mg/l in about 80% of isolates, and no isolate was significantly less sensitive to metconazole among this group. Among the F. graminearum species complex, F. asiaticum and F. graminearum s. str. were identified by PCR-RFLP. The trichothecene chemotypes were determined as 3ADON, 15ADON, or NIV by multiplex PCR. Although species-specific geographical distributions and mycotoxin production characteristics were found, the MIC values to metconazole of both species were distributed within a similar range, and no significant difference in sensitivity was observed between the species or trichothecene chemotypes. Next, CYP51 genes from isolates with different sensitivities to metconazole were amplified by PCR, and their sequences were compared. As a result, it was suggested that the differences in sensitivity to metconazole between the isolates were not due to the substitution of amino acids in CYP51, the target enzyme of DMI. Then, macrospores from isolates with various sensitivities to metconazole were sprayed onto wheat ears, and the efficacy of metconazole was examined. Metconazole showed high control activity against every isolate.
  • Ahmed S. Abdel-Aty
    原稿種別: Original Article
    2010 年 35 巻 4 号 p. 431-440
    発行日: 2010/11/25
    公開日: 2010/11/24
    [早期公開] 公開日: 2010/11/01
    ジャーナル フリー
    Fungicidal activity of six prepared indole derivatives in addition to indole-3-acetic acid and indole-3-butyric acid was in vitro evaluated against Fusarium calmorum, Pythium debarianum, Rhizoctonia solani and Macrofomina phaseoli fungi. The toxicity appeared to be a function of both the treated fungus and the tested concentration. IC50 values were calculated and the structure activity relationship (SAR) was illustrated. 2-Phenylindole and 1-acetylindole-3-butyric acid exhibited persuasive fungicidal activities, so their effects were examined in vivo against polyphenoloxidase (PPO), peroxidase (PO), DNA, RNA and sugar contents. Polyphenoloxidase in R. solani systematically responded to 2-phenylindole concentrations with IC50 value of 80.3 μg/ml. 1-Acetylindole-3-butyric acid was more effective than 2-phenylindole with IC50 values of 41.5 and 80.2 μg/ml comparing with 87.6 and 117.1 μg/ml on F. calmorum and M. phaseoli, respectively. While against P. debarianum, the enzyme was inhibited by 1-acetylindole-3-butyric acid with IC50 equaled 45.6 μg/ml. Effect of the tested compounds on peroxidase was differed among the tested fungi. The peroxidase extracted from P. deparianum was less inhibited. Changing in sugar, DNA and RNA contents of the tested fungi were exhibited that explains the disturbance of the fungal cell physiology and developing deformed and dead cells.
  • Mitsugu Miyamoto, Toshiyuki Katagi
    原稿種別: Original Article
    2010 年 35 巻 4 号 p. 441-446
    発行日: 2010/11/25
    公開日: 2010/11/24
    [早期公開] 公開日: 2010/08/02
    ジャーナル フリー
    Acute toxicity and metabolism of a new insecticide pyridalyl were examined in the larval midge Chironomus yoshimatsui and adult amphipod Hyalella azteca using 14C-labeling. The median lethal concentrations (LC50) of pyridalyl were determined to be 1.1 (48 hr) and 0.015 (96 hr) mg/l for midges and amphipods, respectively, based on its mean measured concentrations in water. Pyridalyl taken up by these organisms underwent ether cleavage at 3,3-dichloroallyloxy moiety followed by conjugation to a significantly higher extent in C. yoshimatsui. The similar acute median lethal body residues (LR50) of pyridalyl estimated for both species from its body residues indicated an insignificant difference in its potential toxicity and therefore, uptake and metabolism behaviors were most likely to play a great role in the apparent toxicity.
  • Masahiro Nishiyama, Yusuke Suzuki, Toshiyuki Katagi
    原稿種別: Original Article
    2010 年 35 巻 4 号 p. 447-455
    発行日: 2010/11/25
    公開日: 2010/11/24
    [早期公開] 公開日: 2010/08/18
    ジャーナル フリー
    Metofluthrin [I, 2,3,5,6-tetrafluoro-4-(methoxymethyl)benzyl (EZ)-(1R,3R)-2,2-dimethyl-3-(prop-1-enyl)cyclopropanecarboxylate] was resistant to hydrolysis at pH 4 and 7 but moderately hydrolyzed with a half-life of 36.8 days at pH 9 and 25°C to form the corresponding acid and alcohol via ester cleavage. The activation energy was estimated to be 105 kJ mol−1 based on the hydrolysis rates at 25–50oC. By continuous exposure to light at >290 nm from a Xenon arc lamp at hydrolytically stable pH 4 and 25°C, I was rapidly photodegraded with half-lives of 1.1–3.4 days, mainly via ester cleavage and successive oxidation followed by mineralization to carbon dioxide. Spectroscopic analyses together with co-chromatography with authentic standards showed that the major degradates having an ester linkage were the aldehyde and carboxylic acid derivatives formed via oxidative cleavage of the prop-1-enyl group together with the diol formed possibly through an oxidative intermediate, such as an epoxide.
  • Takuo Fujisawa, Keiko Ichise-Shibuya, Toshiyuki Katagi
    原稿種別: Original Article
    2010 年 35 巻 4 号 p. 456-463
    発行日: 2010/11/25
    公開日: 2010/11/24
    [早期公開] 公開日: 2010/09/06
    ジャーナル フリー
    Uptake and transformation of phenol and its five derivatives each labeled with 14C were examined in duckweed (Lemna gibba). A kinetic analysis on uptake and metabolic transformation was conducted using the assumed compartments. Positive correlation was observed between log P and the logarithm value of relative uptake rate constant with respect to phenol, and an even higher correlation was obtained against the physico-chemical index, EffTox, where the undissociated fraction of phenol was incorporated. No significant correlation was observed between any of the electronic parameters of the phenol derivatives and the transformation rate. These analyses showed that the uptake of phenols by duckweed is mainly controlled by the hydrophobic profile of the undissociated form. For the metabolic profile, the glycoside conjugate was detected as a typical major metabolite for all phenols and the glutathione conjugate as a unique metabolite for 4-chlorophenol.
  • Archana Upadhyay, Madhuban Gopal, Chitra Srivastava, N. D. Pandey
    原稿種別: Original Article
    2010 年 35 巻 4 号 p. 464-469
    発行日: 2010/11/25
    公開日: 2010/11/24
    [早期公開] 公開日: 2010/11/08
    ジャーナル フリー
    A series of twenty 3,5-disubstituted isoxazoles were synthesized (using conventional and microwave methods) by the reaction of substituted chalcones with hydroxylamine hydrochloride and characterized by TLC, IR, 1H NMR, 13C NMR spectroscopic data and elemental analysis. The compounds were screened for insecticidal activity against the pulse beetle, Callosobruchus chinensis. Compounds 1a (LC50 36 mg L−1), 1d (LC50 110 mg L−1), 2c (LC50 93 mg L−1) and 5b (LC50134 mg L−1) were more effective than the recommended organophosphorous insecticide dichlorvos (2,2-dichlorovinyl dimethyl phosphate) (LC50 155 mg L−1). These compounds provide a lead for designing new substances endowed with insecticidal activity.
  • Akifumi Hosoda, Yudai Hotta, Toshiyuki Isoi, Satomi Amao, Akiyo Ito, K ...
    原稿種別: Original Article
    2010 年 35 巻 4 号 p. 470-478
    発行日: 2010/11/25
    公開日: 2010/11/24
    [早期公開] 公開日: 2010/10/19
    ジャーナル フリー
    電子付録
    To understand the extent of natural adaptation to chemical exposure within a microbial consortium, we scrutinized the dynamic relationship between the microbial diversity and biodegradation capacity of octylphenol polyethoxylates (OPEOn), as representative alkylphenol polyethoxylates (APEOn), using enrichment cultures of various sediments from the Iwata River system. To address the potential of microbes in river sediments to transform a surfactant into endocrine-disrupting chemicals, the ability of the microbes to degrade OPEOn in six different sediment samples was assessed by enrichment culture. In addition, 16S rRNA gene-based denaturing gradient gel electrophoresis (DGGE) analysis was conducted to elucidate the microbial communities before and after enrichment of OPEOn-degrading microbes. Functional gene (adh1) copies were also enumerated to estimate the potential of OPEOn degradation in the six sediment samples. Moreover, microbial communities in three of the six sediment samples were characterized by 16S rRNA-based clone analysis. OPEOn-degrading activity was determined to be present in five of the six enrichment cultures; however, no predominant species could be found by clone analysis. DGGE analysis revealed that the genus Pseudomonas, which contributes to the degradation of OPEOn, was one of the major populations in the enrichment cultures. The adh1 gene enumeration was shown to be relatively high when river flow was relatively slow. These results suggest that potential OPEOn degraders are widely distributed in Iwata River with differences in their history of chemical exposure.
Notes
Society Awards 2010
  • Junetsu Sakai, Ichiro Miura, Masaru Shibata, Norihisa Yonekura, Hideta ...
    原稿種別: Society Awards 2010
    2010 年 35 巻 4 号 p. 488-489
    発行日: 2010/11/25
    公開日: 2010/11/24
    [早期公開] 公開日: 2010/10/19
    ジャーナル フリー
    Benthiavalicarb-isopropyl is a novel fungicide belonged to the carboxylic acid amides (CAA) group. This compound is a highly selective fungicide that has superb preventive and curative effects on late blight and downy mildew caused by plant pathogens that belong to Oomycetes. Benthiavalicarb-isopropyl strongly inhibits mycelia growth, zoosporangia germination and cystospore germination, and also inhibits the sporulation of Phytophthora infestans at a very low concentration. The fungicidal and disease-controlling activities of benthiavalicarb-isopropyl are characterized by its excellent preventive, curative, translaminar, systemic movement, residual activities, its inhibitory activity toward lesion development and its rain fastness. These results suggest that the biological properties of benthiavalicarb-isopropyl contribute to its excellent ability to control several diseases in the field.
  • Masanori Tohnishi, Tetsuyoshi Nishimatsu, Kazuhiko Motoba, Takashi Hir ...
    原稿種別: Society Awards 2010
    2010 年 35 巻 4 号 p. 490-491
    発行日: 2010/11/25
    公開日: 2010/11/24
    [早期公開] 公開日: 2010/11/10
    ジャーナル フリー
    Flubendiamide, a novel class insecticide possessing a unique chemical structure, was discovered by Nihon Nohyaku Co., Ltd., and was registered in Japan in 2007 under the trade name of Phoenix WDG. The compound is not only the first example of 1,2-benzenedicarboxamide insecticides but also the first practical synthetic insecticide with a mode of action as an activator of ryanodine receptors. It shows high and selective activity against lepidopterous insect pests, which leads to excellent efficacy in the field, and excellent safety against non-target organisms, including various beneficial arthropods and natural enemies. These properties suggested the suitability of flubendiamide for integrated pest management (IPM) programs.
  • Shoko Amano, Itsumi Narita
    原稿種別: Society Awards 2010
    2010 年 35 巻 4 号 p. 492-493
    発行日: 2010/11/25
    公開日: 2010/11/24
    [早期公開] 公開日: 2010/10/25
    ジャーナル フリー
    The authors are involved in technical support of the introduction of a self-testing system that analyzes pesticide residues using an immunoassay at agricultural production sites. The practicality of the commercially available ELISA kit was high; however, measurement interference was confirmed in some vegetables. Ultra-filtration and dilution have been proposed to avoid measurement interference. In addition, the user's manual of the ELISA kit has added improvements for production sites. Voluntary inspection of the pesticide residue was evaluated highly by the market, and trust in this production area was gained.
  • Kenjiro Furuta
    原稿種別: Society Awards 2010
    2010 年 35 巻 4 号 p. 494-495
    発行日: 2010/11/25
    公開日: 2010/11/30
    [早期公開] 公開日: 2010/11/12
    ジャーナル フリー
    Juvenile hormone (JH) plays a crucial role in insect development, reproduction and behavior, but its action at the molecular level is not well understood. In this study, the design, synthesis and biological evaluation of novel anti-JH agents were undertaken to obtain more effective chemicals to assist in elucidating the mode of action of JH. Ethyl 4-(2-benzylhexyloxy)benzoate (KF-13) and ethyl 4-[(6-methoxy-2,2-dimethyl-2H-chromen-7-yl)methoxy]benzoate (KF-38) have been found to show not only highly effective precocious metamorphosis-inducing activity in 3rd-instar larvae of the silkworm, Bombyx mori, but also JH activity in allatectomized 4th-instar larvae. To clarify the endocrine basis for precocious metamorphosis induced by KF-13 and KF-38, JH I and 20-hydroxyecdysone (20-E) titers in hemolymph of silkworm larvae treated with KF-13 and KF-38 were quantified by liquid chromatography-mass spectrometry (LC-MS) and LC-MS/MS, respectively. The result indicates that the almost complete absence of JH I during the early stage of 3rd-instar larvae enables the process of precocious metamorphosis.
  • Masahiro Miyashita
    原稿種別: Society Awards 2010
    2010 年 35 巻 4 号 p. 496-498
    発行日: 2010/11/25
    公開日: 2010/11/24
    [早期公開] 公開日: 2010/11/10
    ジャーナル フリー
    Peptides are key molecules in various biological events, and certain types of animals, such as scorpions, have venom, which includes a variety of peptide toxins. Since venoms contain peptides with various structures and activities, they are recognized as “natural libraries” for pesticide and drug discovery. On the other hand, “synthetic libraries” can be readily prepared by utilizing combinatorial chemistry approaches. Millions of peptide sequences generated by this method can be tested for the biological activity of interest using a high-throughput screening system. Here we introduce the isolation and characterization of novel insecticidal peptides from scorpion venom, and plant immunity-activating peptides from synthetic random libraries.
Erratum
PART II (IN JAPANESE)
学会賞受賞論文
英文編掲載報文・短報等の要旨
解説
ミニレビュー
実験技術講座:農薬残留分析編(第4回)
ニュース
シンポジア
エラータ
Keyword Index to Volume 35, 2010
Author Index to Volume 35, 2010
Contents of Volume 35, 2010
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