Forsythiae Fructus is known to have diuretic, anti-bacterial, and anti-inflammatory activities. This study examined the hepatoprotective effects of pinoresinol, a lignan isolated from
Forsythiae Fructus, against carbon tetrachloride (CCl
4)–induced liver injury. Mice were treated intraperitoneally with vehicle or pinoresinol (25, 50, 100, and 200 mg/kg) 30 min before and 2 h after CCl
4 (20
μl/kg) injection. In the vehicle-treated CCl
4 group, serum aminotransferase activities were significantly increased 24 h after CCl
4 injection, and these increases were attenuated by pinoresinol at all doses. Hepatic glutathione contents were significantly decreased and lipid peroxidation was increased after CCl
4 treatment. These changes were attenuated by 50 and 100 mg/kg of pinoresinol. The levels of protein and mRNA expression of inflammatory mediators, including tumor necrosis factor-
α, inducible nitric oxide synthase, and cyclooxygenase-2, were significantly increased after CCl
4 injection; and these increases were attenuated by pinoresinol. Nuclear translocation of nuclear factor-
κB (NF-
κB) and phosphorylation of c-Jun, one of the components of activating protein 1 (AP-1), were inhibited by pinoresinol. Our results suggest that pinoresinol ameliorates CCl
4-induced acute liver injury, and this protection is likely due to anti-oxidative activity and down-regulation of inflammatory mediators through inhibition of NF-
κB and AP-1.
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