Die haploide Chromosomenzahl von Asparagus officinalis beträgt 10. Von 10 Chromosomen sind 6 gross und 4 klein. Die Chromosomenanordnung in der Metaphase der Reduktions-teilung sowie der somatischen Teilung ist nicht ganz unregelmässig; die grossen lagern sich an der Peripherie und die kleinen im Zentrum. Die Längsspaltung für die zweite Teilung ist schon in der Metaphase zu sehen. Das Verhalten der Chromosomen in der Anaphase beider Teilungen ist normal. In der Metaphase der somatischen Teilung ist die paarweise Anordnung der Chromosomen zu sehen. Es gibt kein morphologisch wahrnehmbares Geschlechtschromosom.
1. Some white flower in Japanese Morning Glories (Pharbitis Nil) behaves to a coloured flower as a Mendelian dominant, giving the segregating ratio being in accordance with the ratio 15:1 or 3:1. This dominant white is due to the suppression of the colour, caused by inhibiting factors. 2. The dominant white is coloured in tube and green in stem as well as the recessive white with coloured tube. The ratio of white and coloured flowers in F2 of some crossing between these whites was 61:3. 3. The inhibiting factor concerning the formation of the dominant white is two, each of which acts independently to the factor R, the partner of the factor C being essential to produce the colour in corolla. 4. The factor for the white margined flower is considered as an inhibiting factor by which the factor R in margin-zone of corolla is inhibited its function. Moreover, there occurs the factor to inhibit the factor for the white margin flower, therefore it may be considered as inhibiting factors for inhibiting factors of R. 5. A flower having a pattern shaded off almost white towards the margin from center zone round the tube, is due to the absence of a factor Sf by which the function of R may be supported. The factor Sf is inhibited its function by an inhibiting factor Hf 6. Hence, some patterns are considered as being due to the absence or presence of the factor which acts only to R in some part of corolla as its sapportor or modifier. So far studied, inhibitors to inhibit totally or partially the factor R may be at least seven pairs.
Some such investigators as Osterwalder, Knight, Jost, Correns, East and others have noticed that the pollen tubes of the self incompatible plants grew very slowly in their own stylar tissues and that they were unable to reach the ovaries before the flowers would have wilted, and considered that these might have been the main cause of self incompatibility. The author has studied such a kind of problems taking Petuniaviolacea for his material and obtained almost similar results. 1. Intra-self- and cross-pollinations were made with the self compatible plants. 24 hours after the pollination some of the pollinated pistils were cut off at the distance of 1cm. or 1.5cm. from the stigmas. And the results of the fertilization were compared with those obtained with the flowers, the pistils of which remained entire. The fertilization of the intra-self pollinated flowers was severely injured on account of the cutting, but that of the cross pollinated ones was scarcely affected. These results show us that the pollen tubes grow more rapidly in the case of the cross pollination than that of the intra-selfing. 2. The two kinds of pollinations above mentioned were carried out with the self incompatlble plants. The pistils were fixed at the end of 36 hours after the pollination. They were sectioned longitudinally, stained with cotton blue, and then their microscopic observations were made. The pollen tubes of the cross pollinated flowers were much longer (about 4 times) than those of the intra-self pollinated ones. 3. The pollinated pistils were fixed every 12 hours after the two kinds of pollinations were made with the self incompatible plants, which have been kept in a dark room regulated at 27°C., and the length of the pollen tubes was measured. In the case of the cross pollinated flowers the growth of the pollen tubes was not only rapid but also in an accelerating rate reaching the base of the pistil in 36 hours after pollination. In the intra-self pollinated pistils, however, the velocity of the pollen tube growth was not only very slow but also gradually decreased (Fig. 1, Fig. 2). 4. The intra-self pollination was made with the self incompatible plants as soon as the anthers opened and the styles were fixed when they droped off the flowers. The pollen tubes penetrated the stylar tissues only about one-fifth of the length of the styles. 5. When the intra-self pollination was made with the self incompatible plants, the pollen tubes formed irregular swellings at their tips (Fig. 3). 6. The growth of the pollen tubes was observed with two kinds of 10% sugar solutions: into one of them some tissue-juice of the style of the same flower was mixed, and into the other some juice of a style of a different line was added. The tubes grew more rapidly in the latter than in the formar. It was also noticed that the pollen grains of the self incompatible plants could hardly grow their tubes in the medium mixed with the juice of the style of the same flower (Fig. 4). These results make us to conjecture that there will be some substances which affect the pollen tube growth in the style. Where do such substances come from? What is the relation that exists between such substances and the stigma-secretion? The solution of these problems is underway now at the author′s laboratory, and the results of the experiments pertaining to these problems will be reported in a near future.
Im November-Heft dieser Zeitschrift1), habe ich über, , die in Japan neulich gefundenen Myxomyceten“geschrieben. Vor kurzem habe ich die Ehre gehabt, eine Anzahlvon Schleimpilze zu untersuchen, welche aus dem Biologischen Laboratorium des Kaiserlichen Hofs stammten. Ich fand dabei eine ganz neue Art and Varietät, welche sich folgendermassen diagnostizieren lassen. 1. Clastoderma Debazyianum BLVTT var. imperatoria var. nov. (Tafel 1, fig. 1-3). Plasmodium? Sporangien: gauze Höhe ungefähr 0.8mm., gestielt, rund, Durchmesser 0.3mm., braun (fig. 1&2, ×100). Sporangiumwand: nicht deutlich, zerbrechend, ein kleiner Teil derselben nur am Stiel bleibend. Stiel: an dessen Mitte eine geschwollene Node, wovon der untere Teilrunzlig, dunkelbraun and der obere Teilfeiner und heller ist, als der untere. Columella: braun, kurz und zu Kapillitien verzweigt. Kapillitium: sehr grobes Netz mit nicht so häufig verzweigten Fäden, 2μ dick; die Enden derselben (2 oder 3) begegnen sich an der Ober-fläche des Sporangiums und bilden kleine, hautartige und runde oder polygonale Platten, in der Grösse von 10-15μ10-20μ. Diese hautartigen Platten sind netzartig gemustert. Sporen: hellbraun, glatt, Durchmesser 10μ (fig. 3, ×400). An der Borke einer lebenden Eiche (Quercus acuta THUNB.), Jimmuji bei Zushi, Kanagawa Präfektur. 2. Diderma imperialis sp. nov. (Tafel 1, fig. 4-6). Plasmodium? Sporangien: gesellig, halbkugelig oder niedergedrückt, unterer Teilnabelförmig, Durchmesser ca. 1mm., dunkelbraun [, , Dusky neutral gray''-, , Olivaceous black (3)"]6). Gewöhnlich sitzend, (fig. 5, ×13), aber manchmal gestielt (fig. 4, ×27). Ganze Höhe des gestielten Sporangiums ist ungefähr 0.35mm. Sporangiumwand: einhäutig, hellbraun, mit amorphen Calziumkörnerchen inkrustiert. Stiel: dunkelbraun, zylindrisch oder pfriemenförmig, länglich gestreift, ca. 0.15mm. lang. Columella: nicht vorhanden. Kapillitium: farblos, fein, ärmlich verzweigt, etwa 0.17μ dick, oft mit kleinen Calziumkörnern und winzigen, runden und lichtbrechenden Verdickungen.Sporen: rund hellviolettbraun, warzig, Duchmesser 10μ (fig. 6, ×400). Auf den Blättern von Leucobryum sp., an der lebenden Borke von Cryptomeria japonica DON.; jimmuji bei Zushi, Kanagawa Präfektur.