1. The seeds on an ear not fully ripen differ considerably in the degree of ripeness from one another, even if they seem uniformly developed.
2. Premature germination depends greatly on different ripening stages and on the treatment of seeds after being gathered. Strict determination of the ripening stage is therefore necessary for the germinaiton experiment of the seeds.
3. Seeds detached and laid a week after gathering begin germinating almost immediately, either intact or embryo alone, and are quicker in doing so than those freshly collected seeds, which germinate later and irregularly.
4. In the case of freshly collected seeds, those that are most ripened, germinate quicker than unripened ones, while in the case of seeds stored a week in the laboratory, the nearly reverse is the case, and germination having taken place at the same time.
5. In the case of seeds in the milk stage, freshly collected seeds, containing the largest amount of water, show the least sign of germination for a while, but when their water-contents are reduced to a constant value about a week after picking, they show abrupt signs of germination.
6. The growth of embryos washed of the endosperm juice is much quicker than those with the juice not washed or added.
7. It is presumed that the endosperm juice of seeds in the milk stage contains a germination prohibiting substance which undergoes a change during the decrease of water in the ripening endosperm or by the artificial desiccation, tending to a substance not interfering with the germination.
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