1. The linkage relation of contracted (
ct) to white-2 (
a2) was studied in both cases of coupling and repulsion. The frequency of recombination was 44.0 percent in coupling, 39.5 percent in repulsion.
2. A high linkage was found between
a2 and purple (
pr), though the frequency of recombinations was impossible to calculate on the segregation in F
2. This fact demonstrates
pr to be a member of the contracted linkage group.
3. The occurrence of a close linkage between
a2 and
i1 was respectively observed in both cases of coupling and repulsion.
4. Dragonfly-1 (
dg1) for dragonfly leaf links to
a2, the frequency of recombinations being 23.0 percent.
5. Faint coloured flower-tube are brought about by either one of two genes, termed faintish (
ft) and extended (
e) respectively. The former, faintish belongs to the contracted linkage group, being linked with
dg1, and with 10.5 percent recombination. The latter, extended links to dragonfly-2 (
dg2) of the duplicated linkage group, the frequency of recombinations being 27.1 percent.
6. Depending upon the linkage studies, the following conclusion may be given here. Five genes, newly adding faintish (
ft) and purple (
pr) to three genes-intense-1, white-2, dragonfly-1, belong to the contracted linkage group, and have each loci on the right region of the contracted chromosome, the order of arrangement being probably
i1-a2-pr-ft-dg1.
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