1. In the leaf-cell of
Selaginella uncinata, S. Kraussiana and
Adiantum capillus-veneris the chloroplast always shows a network of chlorophyll when stained with HEIDENHAIN'S iron-alum haematoxylin, after fixation with various fixatives used in the present study.
2. In the leaf-cell of
Adiantum capillus-veneris and in the prothal lium-cell of
Pteris multifida and
Adiantum capillus-veneris the chloroplast stains homogeneously when it contains many starch grains. Sometimes itshows grana in one portion and stains homogeneously in other portions. In the stem-cell of
Selaginella uncinata when the chloroplast is changing into the leucoplast the network disperses and diffuses into the ground substance. The starch grains are formed in various portions of the network. These facts shows the intimate relation between the network of the chloroplast and the process of assimilation.
3. The leucoplast of the rhizophore of
Selaginella uncinata shows various figures when stained with HEIDENHAIN'S iron-alum haematoxylin, according to the fixative used, thus showing its sensitivity to external stimuli.
In conclusion the writer wishes to express his sincere thanks for the valuable aid given by the Director Dr. H. HATTORI of the Tokugawa Institute for Biological Research. He is also particularly indebted to Prof. Y. SINOTO of Tokyo Imperial University for his valuable suggestions and criticism during the course of this work.
The expenses for the carrying out of this study were partly defrayed out of a grant from the Imperial Academy to which body the writer wishes to express his most sincere thanks.
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