The process of salting out of protein in the agaragar column was observed using turbidimetry, the column which had contained protein homogeneously being put in the water bath in which a concentration gradient of ammonium sulfate was established by diffusion (Fig. 1-1), The distribution of turbidity along the column changed with time from P1 to p2, and eventually to P3, which represents the formation of coagulation bands (Fig. 1-2, i, ii and v). These bands correspond to the number of kinds of protein which had been mixed in the agaragar column. The number can be also by the curve obtained by differentiating the curve P1 (Fig. 1-2, iv and v). Some experiments, quantitative titration of protein solution by ammonium sulfate solution etc. (Fig. 3-9), were made in order to confirm the conclusion and to explain the phenomena. But adequate explanation for the formation of coagulation bands awaits further studies. The coagulation band formation can be conveniently used for determining the number of components in a protein mixture. The author has tried to analyse the protein fraction from the seeds of Brassica species (Fig. 2). The method may be called the agarography. The coagulation bands obtained are preservable, at least for months.
1. In the antheridium of Sargassum Horneri, the nuclear divisions are repeated six times, resulting in 64 spermatozoids. 2. This plant has 32 chromosomes in the haploid phase. 3. This plant has centrosome-like bodies at the both poles of the spindle.
In the writer's previous work1), it was reported that the spores of Athyrium niponicum showed evidently in their germination behaviors a typical character of “Long Day Plant”. presenting the highest rate under continuous exposure to light. Then, by using spores of 15 species of Japanese-grown ferns, the writers investigated whether the tendency of “Long Day Plant” observed in the germination of Athyrium spores applies, as a general tendency, to the germination behaviors of many other species of ferns. First, under small intensity of light (10Lux), most of the experimented species were confirmed to belong to a type of “Long Day Plant”. Namely, as illuminating duration was lengthened, germination percentages went up progressively and reached the highest figure under continuous exposure to light. (Fig. 1). Secondly, the spores having a tendency of “Long Day Plant” were exposed to higher intensity of light (100 and 1000Lux). As a result, the following three tendencies were recognized in their germination behaviors. (1). Many species represented by Dryopteris showed a tendency that the higher germination percentage was obtained by a low intensity to light (10lux) than by larger intensities (100Lux and 1000Lux). (Fig. 3). (2). In Spicantopsis and Asplenium, the germination behaviors were not affected by light intensity, but promoted exclusively by the length of illuminating period. (Fig. 4). (3). In Pyrrosia, the germination behavior was the reverse to the tendency of Dryopteris.