1. The distribution of electric potential on the surface of the seed embryo of Vigna sesquipedalis and its changes with the growth stage during 5 days were investigated, using a vacuum tube potentiometer and Zn/ZnSO4/agar non-polarizing electrodes. 2. Influences of light and mechanical stimuli on the resting potential were also examined, and it was succeeded to exclude these disturbing influences practically by an appropriate device in the measurement. 3. The internal structure of the plant and its changes with growth were explored from the histological aspect in relation to the potential distribution. 4. Experimental results thus obtained indicate that the zonal distribution of the elongation velocity in the axial direction coincides neither with the potential distribution itself as has been hitherto accounted simply nor with that of potential gradient, but most sufficiently with that of the 2nd derivative of the potential, as theoretically warranted in the discussion. 5. A working hypothesis with which these facts are to be explained was based upon an assumption that the distribution of resting potential on the surface of the plant body is determined by the spatial distribution of ionic concentration which ought to refer to the functional differentiation of the tissues concerned.
1. Aspartic acid was oxidized both anaerobically and aerobically by the resting cell of Bacillus pumilus. 2. Cyanide inhibited the oxidation of aspartate aerobically but not anaerobically. This indicates that the observed inhibition is due to the reoxidation of the reduced enzyme by molecular oxygen but not to the reduction of the enzyme by its substrate. 3. Mercuric chloride, monoiodoacetic acid, and ferricyanide inhibited the oxidation activity of bacteria these findings suggest that sulfhydryl group might be involved in the oxidation process. 4. The sensitivity of the oxidation process to hydroxylamine, hydrazine and semicarbazide suggests that active carbonyl groups may also be present in the enzymes. 5. From the reaction with pyrophosphate and ferrocyanide it might be concluded that Mg-ion is not essential in this oxidation process. 6. The mechanism of the acceleration by arsenate and ferrocyanide is obscure.