(1) Fertilized eggs of Coccophora Langsdorfii were centrifuged with 1500 times gravity for 20 minutes. As a result, the intracellular materials were stratified into four layers: oil drops in the centripetal edge, plastids with nucleus, clear plasm layer, and then the granule layer in the centrifugal end. (2) The granule layer is composed of microsome-like granules specially stainable with Bismarck brown. (3) Polar staining, with brilliant green or with neutral red, begins to appear in the rhizoid pole after the morphological polarity is determined, while the staining appears uniformly on all over the surface before that stage. The mode of this staining is independent of the axis of the stratification. This phenomenon verifies that the permeability for these dyes is the highest in the rhizoid pole.