1. Application of KCN in a low concentration to the isolated internodal cell of Nitella cultured in vitro results in slight promotion of rhizoid formation. The shooting from the cell was inhibited by KCN in certain concentrations whereas rhizoid formation was not affected. 2. By the treatment with CH2ICOOH and NaF in certain concentrations, rhizoid formation was completely checked whereas shooting was not abated. 3. Auxin in low concentrations promoted the morphogenetic development. The protoplasmic rotation of the cells was also accelerated temporarily or permanently by application of auxin in low concentrations. 4. The optimum pH for the morphogenesis of the cell appears to be around 6.6.
Respiratory activity (QO2) and R. Q. of the ovary tissues of Lilium speciosum isolated at various stages of fertilization were estimated. Histological examinations were conducted in parallel. The results obtained are as follows: 1. QO2 showed little change by the pollination. 2. QO2 increased markedly at the period 90 hours after pollination, when the conjugation of male and female nuclei was observed. 3. The second increase of QO2 came 200 hours after pollination, when free-nuclear divisions took place in the endosperm. 4. Marked increase of R. Q. was observed at the period 90 hours after pollination.
In the present investigation, relations between fermentation and the osmotic pressure inAspergillus niger andSaccharomyces cerevisiae were observed. 1. Though it is difficult to measure the accurate osmotic values, their relative values change in accordance with initial growth phases and fementation phases, as were shown in the figures. 2. InA. niger, falling of the osmotic values is minimum and the citric acid production is maximum, when pH is low. 3. InSaccharomyces, on the contrary, high pH is preferable for the alcoholic fermentation. Falling of the osmotic values is minimum and the alcoholic production is maximum, when pH is higher.
1) Elongating pollen tubes ofCamellia japonica were checked by putting a slice of mature stigma ofLilium longiflorum on their culture plate. From this it is inferred that the stigma contains some substances which inhibit the growth of Camelliapollen tube and the quantity of these substances increases during the maturation of Lilium stigma (Fig. 3). 2) WhenCamelliapollen tube is affected in their growth, the tube elongation and protoplasmic streaming become intermittent, and the “Cap brock “ frequently appears out of shifted from the centre of the tip of pollen tube (Fig. 5, 6). 3) The, separation of the substances in the stigma ofLiliumwhich can inhibit the growth ofCamelliapollen was demonstrated by paper chromatography and pollen germination test. The Rf-value of one of these substances was found to be 0.2-0.25, usingn-butanol, ethanol and water in a ratio of 4: 1: 1 is developer (Fig. 7).
The phosphorylase activity was determined with the growing endosperms of each of the starchy, waxy and sugary races of maize as well as those of the hybrids, starchy×waxyy and starchy×sugary. Changes in the enzyme activity were followed during the period of from 10 to 35 days after pollination. No appreciable difference was found to exist between these different races and also between the hybrids thereof.
1. Changes in the distribution and the amount of starch grains were observed following the development of various organs and tissues in young seedlings of a bean, Vigna sesquipedalis. 2. The amount of starch grains in the cotyledon decreases steadily as germination proceeds; after 24hr. the decrease is especially abrupt. Starch grains are first consumed in the outer portion of the cotyledon and the consumption proceeds then inward, and finally, on the 7th day, starch grains are observable only in the cells around the vascular bundles. 3. In the presumptive abscission region of the cotyledon, there is a marked accumulation of starch grains from the 2nd to the 5th day; on the 5th day the inception of a histologically detectable differentiation of abscission layer of the cotyledon appears. 4. The hypocotyl and the epicotyl possess a definite amount of starch grains at the beginning of germination; from 25 to 30hr. the amount of starch grains becomes minimum; soon after they increase again and on the 2nd day their amount reaches maximum; then they decrease gradually until the starch grains are almost all consumed on the 6th day, except for a few remaining in the starch sheath of the hypocotyl. 5. In the radicle, there is a heavy accumulation of starch grains in the root cap cells and markedly so in the starch sheath of the elongation zone, reaching a maximum on the 2nd day. 6. In the plumule, starch grains are first detectable only in the dorsal part adjacent to the midrib; on the 2nd day they locate throughout the dorsal mesophyll tissue and then they decrease gradually until on the 6th or 7th day a few starch grains are retained only in the cells beneath the midrib.